Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CBP33_RS01740 Genome accession   NZ_CP021359
Coordinates   351707..352750 (-) Length   347 a.a.
NCBI ID   WP_024813830.1    Uniprot ID   A0A258N8A8
Organism   Acidovorax carolinensis strain NA2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 346707..357750
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CBP33_RS01710 (CBP33_01710) - 346845..347261 (+) 417 WP_086911105.1 YraN family protein -
  CBP33_RS01715 (CBP33_01715) - 347362..347961 (+) 600 WP_086911106.1 SIS domain-containing protein -
  CBP33_RS01720 (CBP33_01720) - 347958..348617 (+) 660 WP_086911107.1 BON domain-containing protein -
  CBP33_RS01725 (CBP33_01725) - 348680..349594 (-) 915 WP_086911108.1 NAD(P)-dependent oxidoreductase -
  CBP33_RS01730 (CBP33_01730) pilU 349821..350957 (-) 1137 WP_086911109.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CBP33_RS01735 (CBP33_01735) - 351001..351636 (-) 636 WP_086911110.1 cyclic nucleotide-binding domain-containing protein -
  CBP33_RS01740 (CBP33_01740) pilT 351707..352750 (-) 1044 WP_024813830.1 type IV pilus twitching motility protein PilT Machinery gene
  CBP33_RS01745 (CBP33_01745) - 352788..353504 (+) 717 WP_086911111.1 YggS family pyridoxal phosphate-dependent enzyme -
  CBP33_RS01750 (CBP33_01750) - 353657..354796 (+) 1140 WP_086911112.1 aminotransferase class V-fold PLP-dependent enzyme -
  CBP33_RS01755 (CBP33_01755) - 354866..356173 (+) 1308 WP_086913886.1 DUF445 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38192.98 Da        Isoelectric Point: 6.7743

>NTDB_id=230152 CBP33_RS01740 WP_024813830.1 351707..352750(-) (pilT) [Acidovorax carolinensis strain NA2]
MDITQLLAFSVKNKASDLHLSSGLPPMIRVHGDVRRINVDALDHKTVHAMVYDIMSDSQRKTYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFGDLALKPRGLVLVTGPTGSGKSTTLAAMVNYLNETEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKLKDGSGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSNSVGMQTLDQN
LTDLVRRNIISPAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=230152 CBP33_RS01740 WP_024813830.1 351707..352750(-) (pilT) [Acidovorax carolinensis strain NA2]
GTGGACATTACCCAGTTGCTCGCATTCAGTGTGAAGAACAAGGCTTCCGACCTGCACTTGTCCTCAGGGTTGCCGCCCAT
GATCCGGGTCCACGGGGATGTGCGCCGCATCAATGTGGACGCGCTGGACCACAAAACCGTGCACGCCATGGTGTACGACA
TCATGAGCGACTCGCAGCGCAAGACGTACGAAGAGTTTCTGGAGGTGGACTTCTCGTTTGAGATCGAAGGCCTGGCGCGC
TTTCGCGTCAACGCCTTCAACCAGAACCGCGGCGCCGCGGCCGTATTCCGCACGATTCCGAGCAAAATCCTGACGCTCGA
GCAGCTCAACGCGCCCAAGATCTTCGGCGACCTGGCCCTCAAGCCGCGCGGCCTGGTGCTGGTGACCGGCCCCACGGGTT
CGGGCAAGTCCACCACGCTGGCGGCCATGGTCAACTACCTGAACGAGACCGAATACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAGTTCGTGCACGAGTCCAAGAAGTGCCTGATCAACCAGCGCGAAGTGGGGCCGATGACGCTGTCGTTTGCGGC
CGCCCTGAAGTCCGCGCTGCGCGAAGATCCCGACGCCATCCTGGTGGGCGAAATGCGCGACCTGGAAACCATCCGCCTGG
CCATGACGGCGGCCGAAACCGGCCACCTGGTGTTTGGCACGCTGCACACCTCCAGCGCCGCCAAGACCATCGACCGGATC
ATTGACGTGTTCCCCGCCGAAGAAAAGGAAATGGTCCGCGCCATGCTGTCCGAATCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGCTGAAAGACGGCTCGGGCCGCGTTGCCGCACACGAGATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCAGCAACAGCGTGGGCATGCAGACGCTGGACCAGAAC
CTTACCGATCTGGTGCGGCGCAACATCATCAGCCCCGCAGAAGCCCGCAGCAAGGCCAAGATCCCCGAGAATTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A258N8A8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

76.522

99.424

0.761
  pilT Pseudomonas aeruginosa PAK

76.522

99.424

0.761
  pilT Acinetobacter nosocomialis M2

76.232

99.424

0.758
  pilT Acinetobacter baumannii D1279779

76.232

99.424

0.758
  pilT Acinetobacter baumannii strain A118

76.232

99.424

0.758
  pilT Acinetobacter baylyi ADP1

75.072

99.424

0.746
  pilT Legionella pneumophila strain Lp02

73.451

97.695

0.718
  pilT Legionella pneumophila strain ERS1305867

73.451

97.695

0.718
  pilT Neisseria gonorrhoeae MS11

68.116

99.424

0.677
  pilT Neisseria meningitidis 8013

68.116

99.424

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.155

98.271

0.66
  pilT Vibrio cholerae strain A1552

67.155

98.271

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

99.424

0.496
  pilU Vibrio cholerae strain A1552

44.179

96.542

0.427
  pilU Acinetobacter baylyi ADP1

40.751

99.712

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398

Multiple sequence alignment