Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CBP33_RS01730 Genome accession   NZ_CP021359
Coordinates   349821..350957 (-) Length   378 a.a.
NCBI ID   WP_086911109.1    Uniprot ID   A0A240TPE0
Organism   Acidovorax carolinensis strain NA2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 344821..355957
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CBP33_RS01700 (CBP33_01700) - 344860..345795 (+) 936 WP_086911104.1 lysylphosphatidylglycerol synthase transmembrane domain-containing protein -
  CBP33_RS01705 (CBP33_01705) rsmI 345916..346845 (-) 930 WP_204246687.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  CBP33_RS01710 (CBP33_01710) - 346845..347261 (+) 417 WP_086911105.1 YraN family protein -
  CBP33_RS01715 (CBP33_01715) - 347362..347961 (+) 600 WP_086911106.1 SIS domain-containing protein -
  CBP33_RS01720 (CBP33_01720) - 347958..348617 (+) 660 WP_086911107.1 BON domain-containing protein -
  CBP33_RS01725 (CBP33_01725) - 348680..349594 (-) 915 WP_086911108.1 NAD(P)-dependent oxidoreductase -
  CBP33_RS01730 (CBP33_01730) pilU 349821..350957 (-) 1137 WP_086911109.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CBP33_RS01735 (CBP33_01735) - 351001..351636 (-) 636 WP_086911110.1 cyclic nucleotide-binding domain-containing protein -
  CBP33_RS01740 (CBP33_01740) pilT 351707..352750 (-) 1044 WP_024813830.1 type IV pilus twitching motility protein PilT Machinery gene
  CBP33_RS01745 (CBP33_01745) - 352788..353504 (+) 717 WP_086911111.1 YggS family pyridoxal phosphate-dependent enzyme -
  CBP33_RS01750 (CBP33_01750) - 353657..354796 (+) 1140 WP_086911112.1 aminotransferase class V-fold PLP-dependent enzyme -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41734.09 Da        Isoelectric Point: 6.9078

>NTDB_id=230151 CBP33_RS01730 WP_086911109.1 349821..350957(-) (pilU) [Acidovorax carolinensis strain NA2]
MERDQASKFINDLLKLMVSRNGSDLFITAEFPPAIKVDGKVTKVSPQPLTGTHTLTLARAVMSDKQVADFERTKECNFAI
SPAGIGRFRVNAFIQQGKVGMVLRTIPLTLPTIDGLGVPQVLKEVTMTKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPVEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRAMVSQRLIPKQDGKGRAAAVEVMLNTPLIADLIFKGEVAEIKEVMKKSRNLG
MQTFDQALFDAYEANVISYEDALRNADSLNDLRLQIKLNSQRARSPDLASGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=230151 CBP33_RS01730 WP_086911109.1 349821..350957(-) (pilU) [Acidovorax carolinensis strain NA2]
ATGGAACGCGATCAGGCCAGTAAATTCATCAACGACTTGCTCAAACTGATGGTGAGCCGCAATGGCAGCGACTTGTTCAT
CACAGCCGAGTTTCCGCCGGCCATCAAGGTCGACGGAAAAGTGACCAAGGTATCGCCCCAGCCGCTGACGGGCACCCACA
CCCTCACGCTGGCGCGCGCCGTCATGAGCGACAAGCAGGTGGCCGACTTCGAGCGCACCAAGGAATGCAACTTCGCCATC
TCGCCTGCCGGCATTGGCCGCTTTCGCGTGAACGCCTTCATCCAGCAGGGCAAGGTCGGCATGGTATTGCGGACGATTCC
GCTCACGCTGCCCACCATTGACGGACTGGGCGTGCCGCAGGTTCTCAAGGAAGTGACGATGACCAAGCGCGGCCTGTGCA
TCCTGGTGGGTGCCACGGGCTCGGGCAAGTCCACCACGCTCGCGGCCATGGTGGACTGGCGCAATGAGAACTCGTTTGGT
CACATCATCACGGTGGAAGACCCCGTGGAATTCGTGCACCCGCACAAGAACTGCGTGGTGACGCAGCGCGAAGTGGGCCT
GGACACCGACAGCTGGGAAGCGGCGCTCAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAACATGCGGTGGCCTTCGCCGAAACCGGTCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAAGAGCGCCGCGCCCAGCTGCTGATGGACCTGTCGCTCAACCT
GCGCGCCATGGTGTCGCAGCGACTGATCCCCAAGCAGGATGGCAAGGGCCGGGCTGCGGCGGTGGAGGTCATGCTCAACA
CCCCGCTGATTGCCGACCTGATCTTCAAGGGCGAAGTCGCCGAGATCAAGGAGGTCATGAAAAAGAGCCGCAATCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGATGCCTATGAGGCCAATGTGATCAGCTATGAAGATGCGCTGCGCAACGCCGA
CTCGCTCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCCAGCGCGCCAGGTCGCCCGACCTGGCTTCGGGCACCGAGC
ATTTCGCCATCGTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A240TPE0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.47

92.328

0.595
  pilU Acinetobacter baylyi ADP1

58.263

94.444

0.55
  pilU Vibrio cholerae strain A1552

52.893

96.032

0.508
  pilT Pseudomonas aeruginosa PAK

44.51

89.153

0.397
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.62

89.153

0.389
  pilT Legionella pneumophila strain ERS1305867

43.844

88.095

0.386
  pilT Legionella pneumophila strain Lp02

43.844

88.095

0.386
  pilT Acinetobacter nosocomialis M2

43.844

88.095

0.386
  pilT Acinetobacter baumannii D1279779

43.844

88.095

0.386
  pilT Acinetobacter baumannii strain A118

43.844

88.095

0.386
  pilT Pseudomonas stutzeri DSM 10701

42.73

89.153

0.381
  pilT Acinetobacter baylyi ADP1

43.26

84.392

0.365

Multiple sequence alignment