Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ALW85_RS05045 Genome accession   NC_004757
Coordinates   1051295..1052338 (-) Length   347 a.a.
NCBI ID   WP_011111574.1    Uniprot ID   -
Organism   Nitrosomonas europaea ATCC 19718     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1046295..1057338
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ALW85_RS05030 (NE0962) - 1047032..1048744 (-) 1713 WP_011111571.1 hydroxylamine oxidoreductase -
  ALW85_RS05035 (NE0963) mnmA 1049051..1050139 (+) 1089 WP_011111572.1 tRNA 2-thiouridine(34) synthase MnmA -
  ALW85_RS05040 (NE0964) pilU 1050142..1051278 (-) 1137 WP_011111573.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ALW85_RS05045 (NE0965) pilT 1051295..1052338 (-) 1044 WP_011111574.1 type IV pilus twitching motility protein PilT Machinery gene
  ALW85_RS05050 (NE0966) - 1052535..1053251 (+) 717 WP_011111575.1 YggS family pyridoxal phosphate-dependent enzyme -
  ALW85_RS13555 (NE0967) - 1053220..1053489 (-) 270 WP_011111576.1 YfhL family 4Fe-4S dicluster ferredoxin -
  ALW85_RS05055 (NE0968) coaD 1053509..1053988 (-) 480 WP_011111577.1 pantetheine-phosphate adenylyltransferase -
  ALW85_RS05060 (NE0969) rsmD 1053981..1054532 (-) 552 WP_011111578.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ALW85_RS05065 (NE0970) - 1054542..1055846 (-) 1305 WP_011111579.1 M16 family metallopeptidase -
  ALW85_RS05070 (NE0971) - 1056001..1056420 (+) 420 WP_011111580.1 VOC family protein -
  ALW85_RS05075 (NE0972) - 1056451..1056849 (+) 399 WP_011111581.1 DUF7079 family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38467.25 Da        Isoelectric Point: 7.1151

>NTDB_id=22976 ALW85_RS05045 WP_011111574.1 1051295..1052338(-) (pilT) [Nitrosomonas europaea ATCC 19718]
MNIVELLSFVVKNNASDLHLSAGMPPMIRVHGEIRRINLPALEHKDVHDMVYDIMNDSQRKHYEEHLECDFSFAIPDLAR
FRVNAFNTQRGAASVLRTIPSRVLTLEELKAPKIFAEIAQQPRGVVLVTGPTGSGKSTTLAAMVNDINENQYGHILTLED
PIEFVHESKKCLINQREVGRDTHSFSNALRAALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLRAVISQALLKTKDGKGRVAAHEIMIGTPAIRNLIREGKVAQMYSAIQTGQGVGMQTLDQN
LTDLVKRGVISAVEARTKAMNKDNFRG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=22976 ALW85_RS05045 WP_011111574.1 1051295..1052338(-) (pilT) [Nitrosomonas europaea ATCC 19718]
ATGAATATAGTAGAGTTACTCTCATTTGTGGTCAAGAATAACGCTTCCGATCTTCATTTGTCTGCCGGTATGCCACCTAT
GATCCGGGTGCACGGGGAAATTCGGCGCATCAATCTCCCTGCGCTGGAGCACAAGGACGTGCATGACATGGTGTATGACA
TTATGAACGATAGTCAGCGCAAGCATTATGAAGAACATCTGGAGTGCGATTTTTCGTTTGCTATCCCCGATCTGGCACGT
TTTCGTGTCAACGCTTTCAATACTCAGCGGGGAGCAGCTTCGGTGCTGCGTACCATTCCGTCCAGAGTGCTGACACTCGA
AGAACTGAAGGCGCCAAAAATATTTGCTGAAATTGCGCAACAACCACGCGGTGTGGTGCTTGTTACGGGACCGACCGGAT
CCGGTAAATCCACCACGCTTGCAGCCATGGTCAATGACATCAATGAGAACCAGTATGGCCACATACTGACTCTGGAGGAC
CCGATCGAATTTGTACACGAAAGCAAGAAATGTCTGATCAATCAGCGGGAGGTCGGGCGAGATACACACAGTTTCAGCAA
TGCTCTGCGTGCGGCTTTGAGGGAAGATCCGGACATCATTCTGGTGGGCGAAATGCGGGATCTGGAGACAATTCGTCTGG
CCATGACTGCGGCGGAAACCGGTCATCTGGTTTTTGGTACGCTGCATACCAGTTCGGCTGCCAAGACCATCGACAGGATT
ATTGATGTGTTTCCGGCGGAGGAGAAGGAAATGGTGCGTGCGATGCTGTCCGAATCGCTGCGTGCCGTTATTTCTCAGGC
ACTGCTGAAAACAAAGGATGGTAAGGGTCGGGTGGCTGCTCATGAAATCATGATCGGTACACCGGCTATCCGTAACCTTA
TCCGGGAAGGAAAAGTAGCGCAAATGTATTCGGCTATCCAGACTGGTCAGGGAGTCGGGATGCAAACACTCGATCAGAAT
CTGACAGATCTGGTGAAGCGTGGCGTCATTTCAGCGGTAGAAGCGCGTACCAAAGCTATGAACAAAGATAATTTCCGTGG
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.971

99.424

0.775
  pilT Acinetobacter baumannii D1279779

77.971

99.424

0.775
  pilT Acinetobacter baumannii strain A118

77.971

99.424

0.775
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Pseudomonas aeruginosa PAK

73.623

99.424

0.732
  pilT Pseudomonas stutzeri DSM 10701

72.754

99.424

0.723
  pilT Legionella pneumophila strain Lp02

71.884

99.424

0.715
  pilT Legionella pneumophila strain ERS1305867

71.884

99.424

0.715
  pilT Neisseria gonorrhoeae MS11

68.986

99.424

0.686
  pilT Neisseria meningitidis 8013

68.986

99.424

0.686
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.231

97.406

0.674
  pilT Vibrio cholerae strain A1552

69.231

97.406

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

42.775

99.712

0.427
  pilU Pseudomonas stutzeri DSM 10701

41.908

99.712

0.418
  pilU Acinetobacter baylyi ADP1

40.346

100

0.403

Multiple sequence alignment