Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ALW85_RS05040 Genome accession   NC_004757
Coordinates   1050142..1051278 (-) Length   378 a.a.
NCBI ID   WP_011111573.1    Uniprot ID   Q82VU9
Organism   Nitrosomonas europaea ATCC 19718     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1045142..1056278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ALW85_RS05020 (NE0960) cycA 1045163..1045870 (-) 708 WP_011111569.1 cytochrome c-550 CycA -
  ALW85_RS05025 (NE0961) haoB 1045926..1047035 (-) 1110 WP_011111570.1 hydroxylamine oxidation protein HaoB -
  ALW85_RS05030 (NE0962) - 1047032..1048744 (-) 1713 WP_011111571.1 hydroxylamine oxidoreductase -
  ALW85_RS05035 (NE0963) mnmA 1049051..1050139 (+) 1089 WP_011111572.1 tRNA 2-thiouridine(34) synthase MnmA -
  ALW85_RS05040 (NE0964) pilU 1050142..1051278 (-) 1137 WP_011111573.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ALW85_RS05045 (NE0965) pilT 1051295..1052338 (-) 1044 WP_011111574.1 type IV pilus twitching motility protein PilT Machinery gene
  ALW85_RS05050 (NE0966) - 1052535..1053251 (+) 717 WP_011111575.1 YggS family pyridoxal phosphate-dependent enzyme -
  ALW85_RS13555 (NE0967) - 1053220..1053489 (-) 270 WP_011111576.1 YfhL family 4Fe-4S dicluster ferredoxin -
  ALW85_RS05055 (NE0968) coaD 1053509..1053988 (-) 480 WP_011111577.1 pantetheine-phosphate adenylyltransferase -
  ALW85_RS05060 (NE0969) rsmD 1053981..1054532 (-) 552 WP_011111578.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ALW85_RS05065 (NE0970) - 1054542..1055846 (-) 1305 WP_011111579.1 M16 family metallopeptidase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42083.53 Da        Isoelectric Point: 6.8689

>NTDB_id=22975 ALW85_RS05040 WP_011111573.1 1050142..1051278(-) (pilU) [Nitrosomonas europaea ATCC 19718]
MEKEQALKFMHDLLRLMLSKKASDLFITAGYPPAMKIDGKMTPVTQQLLSSAHTAALARAIMNDKQAVEFESSKECNFAI
HPEGIGRFRVNTFVQQQRTGIVLRTITTKIPNFDDLGLPQVLKEVVMSKRGLVIFVGGTGSGKSTSMAALIGHRNQNSHG
HIITIEDPVEFVHEHINCVVTQREVGVDTESWEAALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLCMGTLHANSA
NQALDRIINFFPEERRAQLLMDLSLNMRALVAQRLIPKKSGSGRAAAIEVMLNSPLISDLIFKGNVHEIKEIMKKSSELG
MQTFDMALFELYENGTISYEDALRNADSMNELRLQIKLHGSEARKISAETNIEHLTIT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=22975 ALW85_RS05040 WP_011111573.1 1050142..1051278(-) (pilU) [Nitrosomonas europaea ATCC 19718]
ATGGAAAAAGAACAGGCACTCAAATTTATGCATGATTTACTGCGGCTGATGCTTAGTAAAAAGGCTTCAGATTTGTTCAT
TACGGCCGGTTATCCTCCGGCAATGAAGATAGACGGCAAAATGACACCTGTCACACAGCAGCTACTTTCTTCTGCCCATA
CTGCAGCGCTGGCGCGTGCCATCATGAACGATAAACAGGCGGTGGAGTTCGAGTCCAGTAAGGAATGTAATTTTGCAATT
CATCCGGAAGGAATCGGCCGTTTTCGCGTCAATACTTTCGTACAACAGCAACGAACAGGAATCGTTTTGCGGACGATCAC
CACGAAGATTCCAAATTTCGATGATCTTGGGCTGCCGCAGGTTCTGAAAGAAGTCGTCATGAGCAAACGTGGTCTGGTGA
TTTTTGTCGGTGGAACCGGTTCGGGAAAATCCACCTCGATGGCGGCACTGATTGGCCATCGCAACCAGAATAGCCATGGC
CATATCATCACTATTGAAGATCCGGTTGAATTTGTACATGAACATATCAATTGTGTCGTTACCCAGCGGGAAGTCGGAGT
TGATACGGAATCGTGGGAAGCTGCATTGAAGAATACATTGCGGCAGGCGCCCGATGTCATTCTGATTGGTGAAATCCGGG
ATCGAGAAACAATGGAGCACGCCATTGCCTTTGCTGAGACCGGGCATTTGTGTATGGGTACCCTGCATGCAAACAGCGCC
AATCAGGCGCTTGATCGCATTATTAATTTCTTCCCGGAAGAACGGCGTGCTCAGTTGCTGATGGACCTGTCACTGAATAT
GCGGGCATTGGTCGCGCAAAGGCTGATTCCCAAAAAATCAGGCAGCGGGCGTGCTGCGGCAATCGAAGTAATGCTCAATT
CTCCACTGATTTCTGATCTGATATTCAAGGGAAATGTGCATGAAATCAAGGAAATAATGAAAAAATCAAGTGAACTGGGG
ATGCAAACTTTTGATATGGCGCTGTTTGAACTCTATGAGAATGGCACAATTTCCTATGAAGATGCGCTTCGTAATGCTGA
TTCCATGAATGAATTGAGGCTGCAAATCAAATTGCACGGATCAGAAGCCCGGAAAATCAGTGCAGAGACGAATATCGAAC
ATCTGACGATTACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(20-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q82VU9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.637

96.296

0.603
  pilU Acinetobacter baylyi ADP1

60.224

94.444

0.569
  pilU Vibrio cholerae strain A1552

56.891

90.212

0.513
  pilT Pseudomonas aeruginosa PAK

43.582

88.624

0.386
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.687

88.624

0.378
  pilT Legionella pneumophila strain Lp02

42.598

87.566

0.373
  pilT Legionella pneumophila strain ERS1305867

42.598

87.566

0.373
  pilT Pseudomonas stutzeri DSM 10701

41.791

88.624

0.37

Multiple sequence alignment