Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SO_RS15625 Genome accession   NC_004347
Coordinates   3495999..3497036 (-) Length   345 a.a.
NCBI ID   WP_011073221.1    Uniprot ID   -
Organism   Shewanella oneidensis MR-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3490999..3502036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SO_RS15595 (SO_3345) yciH 3491375..3491704 (+) 330 WP_011073215.1 stress response translation initiation inhibitor YciH -
  SO_RS15600 (SO_3346) - 3492040..3492603 (+) 564 WP_011073216.1 YqgE/AlgH family protein -
  SO_RS15605 (SO_3347) ruvX 3492631..3493056 (+) 426 WP_011073217.1 Holliday junction resolvase RuvX -
  SO_RS15610 (SO_3348) hemH 3493119..3494102 (-) 984 WP_011073218.1 ferrochelatase -
  SO_RS15615 (SO_3349) - 3494173..3494703 (-) 531 WP_405130463.1 glutathione peroxidase -
  SO_RS15620 (SO_3350) pilU 3494877..3495989 (-) 1113 WP_011073220.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SO_RS15625 (SO_3351) pilT 3495999..3497036 (-) 1038 WP_011073221.1 type IV pilus twitching motility protein PilT Machinery gene
  SO_RS15630 (SO_3352) - 3497072..3497785 (+) 714 WP_011073222.1 YggS family pyridoxal phosphate-dependent enzyme -
  SO_RS15635 (SO_3354) proC 3498010..3498828 (+) 819 WP_011073223.1 pyrroline-5-carboxylate reductase -
  SO_RS15640 (SO_3355) - 3498905..3499453 (+) 549 WP_011073224.1 YggT family protein -
  SO_RS15645 (SO_3356) yggU 3499453..3499743 (+) 291 WP_011073225.1 DUF167 family protein YggU -
  SO_RS15650 (SO_3357) - 3499811..3500245 (+) 435 WP_011073226.1 DUF4426 domain-containing protein -
  SO_RS15655 (SO_3358) rdgB 3500363..3500980 (+) 618 WP_011073227.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38326.00 Da        Isoelectric Point: 6.9096

>NTDB_id=22405 SO_RS15625 WP_011073221.1 3495999..3497036(-) (pilT) [Shewanella oneidensis MR-1]
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDFEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKISSFPRGLVLVTGPTGSGKSTTLAAMVDYINENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMAKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=22405 SO_RS15625 WP_011073221.1 3495999..3497036(-) (pilT) [Shewanella oneidensis MR-1]
ATGGAAATCACTGAGTTATTAGCCTTTAGTGTAAAACACAAAGCCTCGGATCTACACCTCTCTGCAGGGATATCTCCCAT
GATCCGTGTCGACGGTGAAGTGAGAAAAATCAATCTGCCCGCGCTCGATCATCAGGGTGTACACAGCCTTGTGTACGACA
TTATGAATGATAAACAGCGTAAGGACTTTGAAGAACATTTAGAAATCGACTTTTCCTTTGAAGTCCCTAACTTAGCGCGT
TTCCGTGTGAACGCCTTTAACCAATCTCGAGGTGCAGCTGCGGTATTTCGTACCATTCCAAGTGATATTTTGTCACTCGA
GCAGTTAGGCGCCCCTGAAATTTTTAAAAAAATCTCCAGTTTTCCCCGTGGCTTAGTGTTAGTCACTGGACCAACTGGTT
CGGGTAAGAGTACCACACTTGCGGCCATGGTGGATTACATCAATGAAAACCGTCATGATCATATCCTAACCATTGAAGAT
CCAATTGAATTCGTTCATCAAAATAAGCAATGTTTGATTAACCAACGGGAAGTGCATCGTCATACCCACAGTTTTAACGC
CGCATTGAGAAGTGCGTTGCGTGAAGACCCTGACGTCATCCTCGTCGGCGAGATGCGTGACCTTGAAACCATTCGACTTG
CGATGACCGCGGCCGAAACGGGTCACTTAGTATTCGGTACCTTGCATACCACCTCGGCAGCTAAGACTATCGACCGTGTG
GTTGACGTTTTCCCCGCTGGCGAAAAAGACATGGTACGTACCATGTTATCGGAATCATTACAGGCTGTTATCTCGCAAAC
TCTGATTAAGAAAATCGGCGGTGGTCGTGTTGCTGCCCACGAAATTATGATGGGTACGCCCGCTATCCGTAACCTTATCC
GTGAAGATAAAGTGGCGCAAATGTACTCTGCTATCCAAACGGGGATGGCCCATGGTATGCAAACTCTTGAACAGTGTCTG
CAAAACTTAGTTAACCGTGGCCTCATTACCCGAGAAGATGCCATGGCGAAAAGTTCAAACAAACAAGCGACGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

80.409

99.13

0.797
  pilT Acinetobacter baumannii strain A118

80.409

99.13

0.797
  pilT Acinetobacter nosocomialis M2

80.409

99.13

0.797
  pilT Pseudomonas aeruginosa PAK

79.586

97.971

0.78
  pilT Acinetobacter baylyi ADP1

77.843

99.42

0.774
  pilT Pseudomonas stutzeri DSM 10701

77.485

99.13

0.768
  pilT Legionella pneumophila strain Lp02

74.78

98.841

0.739
  pilT Legionella pneumophila strain ERS1305867

74.78

98.841

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.107

97.681

0.704
  pilT Vibrio cholerae strain A1552

72.107

97.681

0.704
  pilT Neisseria meningitidis 8013

69.477

99.71

0.693
  pilT Neisseria gonorrhoeae MS11

69.186

99.71

0.69
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.853

98.261

0.49
  pilU Pseudomonas stutzeri DSM 10701

41.983

99.42

0.417
  pilU Vibrio cholerae strain A1552

41.399

99.42

0.412
  pilU Acinetobacter baylyi ADP1

39.763

97.681

0.388

Multiple sequence alignment