Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SO_RS15620 Genome accession   NC_004347
Coordinates   3494877..3495989 (-) Length   370 a.a.
NCBI ID   WP_011073220.1    Uniprot ID   Q8EBZ5
Organism   Shewanella oneidensis MR-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3489877..3500989
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SO_RS15585 (SO_3343) - 3490169..3490729 (-) 561 WP_011073213.1 DUF4136 domain-containing protein -
  SO_RS15590 (SO_3344) - 3490910..3491224 (+) 315 WP_011073214.1 hypothetical protein -
  SO_RS15595 (SO_3345) yciH 3491375..3491704 (+) 330 WP_011073215.1 stress response translation initiation inhibitor YciH -
  SO_RS15600 (SO_3346) - 3492040..3492603 (+) 564 WP_011073216.1 YqgE/AlgH family protein -
  SO_RS15605 (SO_3347) ruvX 3492631..3493056 (+) 426 WP_011073217.1 Holliday junction resolvase RuvX -
  SO_RS15610 (SO_3348) hemH 3493119..3494102 (-) 984 WP_011073218.1 ferrochelatase -
  SO_RS15615 (SO_3349) - 3494173..3494703 (-) 531 WP_405130463.1 glutathione peroxidase -
  SO_RS15620 (SO_3350) pilU 3494877..3495989 (-) 1113 WP_011073220.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SO_RS15625 (SO_3351) pilT 3495999..3497036 (-) 1038 WP_011073221.1 type IV pilus twitching motility protein PilT Machinery gene
  SO_RS15630 (SO_3352) - 3497072..3497785 (+) 714 WP_011073222.1 YggS family pyridoxal phosphate-dependent enzyme -
  SO_RS15635 (SO_3354) proC 3498010..3498828 (+) 819 WP_011073223.1 pyrroline-5-carboxylate reductase -
  SO_RS15640 (SO_3355) - 3498905..3499453 (+) 549 WP_011073224.1 YggT family protein -
  SO_RS15645 (SO_3356) yggU 3499453..3499743 (+) 291 WP_011073225.1 DUF167 family protein YggU -
  SO_RS15650 (SO_3357) - 3499811..3500245 (+) 435 WP_011073226.1 DUF4426 domain-containing protein -
  SO_RS15655 (SO_3358) rdgB 3500363..3500980 (+) 618 WP_011073227.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41227.48 Da        Isoelectric Point: 6.3725

>NTDB_id=22404 SO_RS15620 WP_011073220.1 3494877..3495989(-) (pilU) [Shewanella oneidensis MR-1]
MDVRPFLKVMVERKASDLFITAGFPPSAKIDGELRPLAESIFTPAQSLDFVESVMTEAQKKEFHTTRECNFAFAVKELGR
FRVSAFWQRESPGCVMRRIETKIPEVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNSHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLQLYVDGEISYADALHHADSPNDLRLMIKLQNKEPPSSSFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=22404 SO_RS15620 WP_011073220.1 3494877..3495989(-) (pilU) [Shewanella oneidensis MR-1]
ATGGATGTCCGTCCGTTTTTAAAAGTCATGGTGGAGCGTAAAGCCTCGGACTTGTTTATTACCGCAGGTTTTCCGCCGAG
CGCCAAAATCGATGGTGAGTTACGTCCACTTGCTGAGAGTATATTTACGCCTGCACAATCGTTAGATTTTGTTGAGTCCG
TGATGACCGAGGCACAGAAAAAGGAATTTCACACTACCCGTGAATGTAACTTTGCATTTGCCGTTAAAGAGTTAGGGCGT
TTTCGTGTGAGTGCATTTTGGCAGCGTGAATCTCCTGGCTGTGTAATGCGGCGGATTGAGACTAAAATCCCAGAAGTCGA
TGACTTAAAACTGCCGCCCATATTAAAAGATCTGGTGATGAGTAAGCGTGGACTTATCATCATGGTGGGGGGAACGGGTA
CAGGTAAGTCGACCTCCTTGGCGGCGTTAGTGGGTTATCGTAATTCCCATGCCCGTGGGCATATTCTGACCATTGAAGAT
CCGGTGGAGTTTGTTCACGATCATCGAAAAAGTATCATTACTCAACGTGAGGTGGGGATTGATACAGAGTCCTTTGACGC
AGCGCTAAAGAGTTCATTGCGTCAGGCGCCCGATGTGATTCTCATTGGTGAAATTCGCACCCAAGAAACCATGGAATTTG
CGCTCTCCTTCGCTGAAACAGGCCATCTGTGTATGGCAACACTGCACGCCAACAACGCCAACCAAGCCTTAGATCGGATT
ATGCATTTAGTCCCAGAGAGTAAACACCAACAGCTATTATTCGATTTGTCACTCAACCTTCGTGGTATTGTGGCGCAGCA
ACTGGTACCCAAGGTCGATGGTACAGGACGGCGGGCAGCTATTGAGGTATTGATCAATACCCCAAGGGTGGCAAGCTTGA
TTGCTAAAAATGAGCTGCATTTACTCAAAGAAACCATGGCGAAATCCAACGAGCAAGGAATGCAGACCTTCGACCAAGCC
TTGTTACAGCTTTATGTTGACGGGGAAATCAGTTACGCCGATGCGCTTCACCATGCTGACTCACCAAACGACTTGCGCTT
GATGATCAAACTGCAAAATAAAGAACCTCCCAGTTCGAGTTTTATGGAAGGCGTGACCTTGGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8EBZ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.377

100

0.605
  pilU Vibrio cholerae strain A1552

58.649

100

0.586
  pilU Acinetobacter baylyi ADP1

61.08

95.135

0.581
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.58

93.243

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.683

88.649

0.378
  pilT Vibrio cholerae strain A1552

42.683

88.649

0.378
  pilT Legionella pneumophila strain Lp02

41.088

89.459

0.368
  pilT Legionella pneumophila strain ERS1305867

41.088

89.459

0.368
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362

Multiple sequence alignment