Detailed information NTG0224007

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: B6N50_RS04260
• Genome accession: NZ_CP020543
• Coordinates: 829672..830652 (+)
• Length: 326 a.a.
• NCBI ID: WP_001295381.1
• Uniprot ID: A0A061K2W4
• Organism: Escherichia coli C
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 824672..835652

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B6N50_RS04230 (B6N50_04230)	yggM	825187..826194 (+)	1008	WP_000745217.1	DUF1202 family protein	-
B6N50_RS04235 (B6N50_04235)	hemW	826349..827485 (-)	1137	WP_000239928.1	radical SAM family heme chaperone HemW	-
B6N50_RS04240 (B6N50_04240)	rdgB	827478..828071 (-)	594	WP_081508777.1	RdgB/HAM1 family non-canonical purine NTP pyrophosphatase	-
B6N50_RS04245 (B6N50_04245)	yggU	828079..828369 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
B6N50_RS04250 (B6N50_04250)	yggT	828366..828932 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
B6N50_RS04255 (B6N50_04255)	yggS	828950..829654 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
B6N50_RS04260 (B6N50_04260)	pilT	829672..830652 (+)	981	WP_001295381.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
B6N50_RS24205	-	830701..830829 (+)	129	WP_001278270.1	hypothetical protein	-
B6N50_RS04265 (B6N50_04265)	ruvX	830836..831252 (-)	417	WP_000017106.1	Holliday junction resolvase RuvX	-
B6N50_RS04270 (B6N50_04270)	yqgE	831252..831815 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
B6N50_RS04275 (B6N50_04275)	gshB	831924..832874 (-)	951	WP_000593273.1	glutathione synthase	-
B6N50_RS04280 (B6N50_04280)	rsmE	832887..833618 (-)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
B6N50_RS04285 (B6N50_04285)	endA	833698..834405 (-)	708	WP_000286500.1	deoxyribonuclease I	-
B6N50_RS04290 (B6N50_04290)	yggI	834500..834997 (-)	498	WP_001300769.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35926.14 Da
• Isoelectric Point: 5.7654

>NTDB_id=224007 B6N50_RS04260 WP_001295381.1 829672..830652(+) (pilT) [Escherichia coli C]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGMILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=224007 B6N50_RS04260 WP_001295381.1 829672..830652(+) (pilT) [Escherichia coli C]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCATGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

3D structure

• AlphaFold DB: A0A061K2W4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Neisseria meningitidis 8013	49.085	100	0.494
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	48.93	100	0.491
pilT	Vibrio cholerae strain A1552	48.93	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.78	100	0.491
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.177	100	0.463
pilT	Acinetobacter baylyi ADP1	45.872	100	0.46
pilT	Pseudomonas aeruginosa PAK	45.566	100	0.457
pilT	Legionella pneumophila strain ERS1305867	45.912	97.546	0.448
pilT	Legionella pneumophila strain Lp02	45.912	97.546	0.448
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.824	97.546	0.408
pilU	Vibrio cholerae strain A1552	40.062	98.773	0.396
pilU	Pseudomonas stutzeri DSM 10701	37.273	100	0.377
pilU	Acinetobacter baylyi ADP1	37.048	100	0.377
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362