Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CXW56_RS15740 Genome accession   NZ_CP025916
Coordinates   3186219..3187199 (+) Length   326 a.a.
NCBI ID   WP_001055618.1    Uniprot ID   -
Organism   Escherichia coli strain 120899     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3181219..3192199
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CXW56_RS15710 - 3181597..3182583 (-) 987 WP_000784004.1 TRAP transporter substrate-binding protein -
  CXW56_RS15715 hemW 3182896..3184032 (-) 1137 WP_000239948.1 radical SAM family heme chaperone HemW -
  CXW56_RS15720 rdgB 3184025..3184618 (-) 594 WP_001174744.1 XTP/dITP diphosphatase -
  CXW56_RS15725 yggU 3184626..3184916 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  CXW56_RS15730 yggT 3184913..3185479 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  CXW56_RS15735 yggS 3185497..3186201 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  CXW56_RS15740 pilT 3186219..3187199 (+) 981 WP_001055618.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CXW56_RS15745 ruvX 3187375..3187791 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  CXW56_RS15750 yqgE 3187791..3188354 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  CXW56_RS15755 gshB 3188463..3189413 (-) 951 WP_000593273.1 glutathione synthase -
  CXW56_RS15760 rsmE 3189426..3190157 (-) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CXW56_RS15765 endA 3190237..3190944 (-) 708 WP_000286500.1 deoxyribonuclease I -
  CXW56_RS15770 yggI 3191039..3191536 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35899.09 Da        Isoelectric Point: 5.5860

>NTDB_id=221171 CXW56_RS15740 WP_001055618.1 3186219..3187199(+) (pilT) [Escherichia coli strain 120899]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=221171 CXW56_RS15740 WP_001055618.1 3186219..3187199(+) (pilT) [Escherichia coli strain 120899]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362

Multiple sequence alignment