Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   BVH74_RS08525 Genome accession   NZ_CP020100
Coordinates   1855640..1856071 (+) Length   143 a.a.
NCBI ID   WP_080049644.1    Uniprot ID   A0A1V0B4H1
Organism   Halopseudomonas phragmitis strain S-6-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1850640..1861071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVH74_RS08505 (BVH74_08505) coaE 1850718..1851323 (-) 606 WP_080049640.1 dephospho-CoA kinase -
  BVH74_RS08510 (BVH74_08510) pilD 1851328..1852200 (-) 873 WP_080049641.1 A24 family peptidase Machinery gene
  BVH74_RS08515 (BVH74_08515) pilC 1852202..1853458 (-) 1257 WP_080049642.1 type II secretion system F family protein Machinery gene
  BVH74_RS08520 (BVH74_08520) pilB 1853462..1855165 (-) 1704 WP_080049643.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BVH74_RS08525 (BVH74_08525) pilA/pilA1 1855640..1856071 (+) 432 WP_080049644.1 pilin Machinery gene
  BVH74_RS18770 - 1856220..1856474 (+) 255 WP_155121702.1 hypothetical protein -
  BVH74_RS08530 (BVH74_08530) - 1856627..1857154 (-) 528 WP_080049645.1 GspH/FimT family pseudopilin -
  BVH74_RS08535 (BVH74_08535) - 1857151..1860213 (-) 3063 WP_080049646.1 pilus assembly protein -
  BVH74_RS08540 (BVH74_08540) - 1860237..1860722 (-) 486 WP_080049647.1 PilX N-terminal domain-containing pilus assembly protein -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14887.81 Da        Isoelectric Point: 3.9552

>NTDB_id=221092 BVH74_RS08525 WP_080049644.1 1855640..1856071(+) (pilA/pilA1) [Halopseudomonas phragmitis strain S-6-2]
MKAQKGFTLIELMIVVAIIGILAAIALPAYQDYTGRAQASEALSATAGLRADIAVYLSEEGALPGPGDSTTLDDAIADLE
GKYFNAGSVSLLADGVLQVDFDSGVHSGNNMTLTPIESGAGNQIARWECDDLDPKFLPSACRP

Nucleotide


Download         Length: 432 bp        

>NTDB_id=221092 BVH74_RS08525 WP_080049644.1 1855640..1856071(+) (pilA/pilA1) [Halopseudomonas phragmitis strain S-6-2]
ATGAAGGCTCAAAAAGGCTTTACTTTGATCGAACTGATGATCGTTGTTGCGATCATCGGTATTCTGGCTGCCATCGCGCT
GCCGGCTTATCAGGACTACACTGGCCGTGCTCAGGCTTCTGAGGCTTTGAGCGCTACTGCTGGTCTTCGTGCTGATATTG
CAGTTTATTTGTCAGAGGAAGGTGCTCTCCCTGGGCCCGGTGACTCCACCACTTTGGATGATGCTATTGCTGACTTGGAA
GGTAAATACTTCAATGCTGGTAGCGTGAGTCTTCTGGCTGACGGCGTCTTGCAGGTCGATTTTGATTCGGGCGTGCACTC
TGGAAATAACATGACTCTTACTCCCATCGAAAGTGGCGCAGGTAATCAAATTGCACGTTGGGAGTGTGACGATCTGGATC
CGAAGTTCCTGCCCTCGGCTTGCCGCCCCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(36-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1V0B4H1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

46.667

100

0.49
  comP Acinetobacter baylyi ADP1

43.333

100

0.455
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.558

100

0.448
  pilE Neisseria gonorrhoeae MS11

36.047

100

0.434
  pilE Neisseria gonorrhoeae strain FA1090

38.608

100

0.427
  pilA Pseudomonas aeruginosa PAK

38.065

100

0.413
  pilA2 Legionella pneumophila str. Paris

39.437

99.301

0.392
  pilA2 Legionella pneumophila strain ERS1305867

39.437

99.301

0.392
  pilA Acinetobacter baumannii strain A118

36.913

100

0.385
  pilA Glaesserella parasuis strain SC1401

38.298

98.601

0.378
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.552

100

0.371
  pilA Vibrio cholerae strain A1552

35.616

100

0.364
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

35.616

100

0.364
  pilA Vibrio cholerae C6706

35.616

100

0.364

Multiple sequence alignment