Detailed information NTG0221089

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: BVH74_RS08510
• Genome accession: NZ_CP020100
• Coordinates: 1851328..1852200 (-)
• Length: 290 a.a.
• NCBI ID: WP_080049641.1
• Uniprot ID: A0A1V0B4D3
• Organism: Halopseudomonas phragmitis strain S-6-2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1846328..1857200

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BVH74_RS08480 (BVH74_08480)	lspA	1847288..1847782 (+)	495	WP_080049635.1	signal peptidase II	-
BVH74_RS08485 (BVH74_08485)	fkpB	1847808..1848239 (+)	432	WP_080049636.1	FKBP-type peptidyl-prolyl cis-trans isomerase	-
BVH74_RS08490 (BVH74_08490)	ispH	1848401..1849348 (+)	948	WP_080049637.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
BVH74_RS08495 (BVH74_08495)	-	1849456..1850388 (-)	933	WP_080049638.1	Nudix family hydrolase	-
BVH74_RS08500 (BVH74_08500)	yacG	1850530..1850721 (-)	192	WP_080049639.1	DNA gyrase inhibitor YacG	-
BVH74_RS08505 (BVH74_08505)	coaE	1850718..1851323 (-)	606	WP_080049640.1	dephospho-CoA kinase	-
BVH74_RS08510 (BVH74_08510)	pilD	1851328..1852200 (-)	873	WP_080049641.1	A24 family peptidase	Machinery gene
BVH74_RS08515 (BVH74_08515)	pilC	1852202..1853458 (-)	1257	WP_080049642.1	type II secretion system F family protein	Machinery gene
BVH74_RS08520 (BVH74_08520)	pilB	1853462..1855165 (-)	1704	WP_080049643.1	type IV-A pilus assembly ATPase PilB	Machinery gene
BVH74_RS08525 (BVH74_08525)	pilA/pilA1	1855640..1856071 (+)	432	WP_080049644.1	pilin	Machinery gene
BVH74_RS18770	-	1856220..1856474 (+)	255	WP_155121702.1	hypothetical protein	-
BVH74_RS08530 (BVH74_08530)	-	1856627..1857154 (-)	528	WP_080049645.1	GspH/FimT family pseudopilin	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32121.38 Da
• Isoelectric Point: 7.0080

>NTDB_id=221089 BVH74_RS08510 WP_080049641.1 1851328..1852200(-) (pilD) [Halopseudomonas phragmitis strain S-6-2]
MPLLDYLASHVLAFVLLCGLLGLIVGSFLNVVIHRLPIMLERDWRRQCQEMLEPEVEQPPLPTYNLILPHSHCPHCQSEI
KPWQNLPVISYLILRGRCGHCKERISPRYPLVELLTGGLSMLIAWQLGFGWAAAAMLLLTWALISLSLIDADTQLLPDVI
VLPMLWLGLIVNSFGLFTDLGTALWGAVLGYLSLWSVYWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQVLPLTILLSSLV
GAILGIIILKTRGQSNTTPLPFGPYLAIAGWIALIWGDTITSGYLKFAGF

Nucleotide

• Length: 873 bp

>NTDB_id=221089 BVH74_RS08510 WP_080049641.1 1851328..1852200(-) (pilD) [Halopseudomonas phragmitis strain S-6-2]
ATGCCGCTTCTCGATTATCTGGCCAGCCATGTGCTGGCCTTTGTTTTGCTTTGCGGATTACTCGGGCTGATTGTTGGCAG
CTTCCTCAACGTGGTGATTCATCGCCTGCCCATCATGCTTGAGCGCGATTGGCGTCGGCAATGCCAGGAAATGCTCGAAC
CCGAGGTTGAACAGCCACCGCTGCCAACCTACAACCTGATTCTGCCGCATTCACACTGCCCGCACTGCCAGAGCGAAATC
AAACCCTGGCAGAACCTGCCGGTAATCAGCTACCTGATTCTGCGCGGCCGCTGTGGCCACTGTAAGGAGCGCATCAGCCC
ACGCTACCCGCTGGTGGAGCTGCTGACCGGTGGATTATCAATGCTGATAGCCTGGCAGCTCGGCTTCGGCTGGGCCGCCG
CCGCCATGCTGCTGCTGACCTGGGCACTGATCAGCCTCAGCCTTATCGATGCCGACACCCAGTTGCTGCCCGATGTAATA
GTGCTGCCAATGCTGTGGCTGGGCCTGATCGTCAACAGCTTTGGCCTGTTCACCGATCTGGGCACTGCCCTGTGGGGTGC
GGTACTTGGCTACCTGAGCCTGTGGTCGGTGTACTGGCTGTTCAAGTTGGTGACCGGCAAGGAAGGCATGGGTTATGGCG
ACTTCAAGCTGCTGGCCATGCTCGGCGCCTGGGGCGGCTGGCAAGTGCTGCCCCTGACCATTCTGTTGTCTTCACTGGTG
GGCGCCATTCTCGGCATCATCATCCTCAAGACCCGCGGCCAATCCAACACCACCCCCCTCCCCTTCGGCCCCTACCTGGC
CATTGCCGGCTGGATTGCGTTGATCTGGGGTGACACAATAACCAGTGGATATCTCAAGTTCGCTGGATTTTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (138-246)

3D structure

• AlphaFold DB: A0A1V0B4D3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	52.281	98.276	0.514
pilD	Vibrio cholerae strain A1552	54.212	94.138	0.51
pilD	Acinetobacter nosocomialis M2	51.579	98.276	0.507
pilD	Vibrio campbellii strain DS40M4	52.381	94.138	0.493
pilD	Neisseria gonorrhoeae MS11	50	91.724	0.459