Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BVH74_RS08520 Genome accession   NZ_CP020100
Coordinates   1853462..1855165 (-) Length   567 a.a.
NCBI ID   WP_080049643.1    Uniprot ID   A0A1V0B4G5
Organism   Halopseudomonas phragmitis strain S-6-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1848462..1860165
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVH74_RS08495 (BVH74_08495) - 1849456..1850388 (-) 933 WP_080049638.1 Nudix family hydrolase -
  BVH74_RS08500 (BVH74_08500) yacG 1850530..1850721 (-) 192 WP_080049639.1 DNA gyrase inhibitor YacG -
  BVH74_RS08505 (BVH74_08505) coaE 1850718..1851323 (-) 606 WP_080049640.1 dephospho-CoA kinase -
  BVH74_RS08510 (BVH74_08510) pilD 1851328..1852200 (-) 873 WP_080049641.1 A24 family peptidase Machinery gene
  BVH74_RS08515 (BVH74_08515) pilC 1852202..1853458 (-) 1257 WP_080049642.1 type II secretion system F family protein Machinery gene
  BVH74_RS08520 (BVH74_08520) pilB 1853462..1855165 (-) 1704 WP_080049643.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BVH74_RS08525 (BVH74_08525) pilA/pilA1 1855640..1856071 (+) 432 WP_080049644.1 pilin Machinery gene
  BVH74_RS18770 - 1856220..1856474 (+) 255 WP_155121702.1 hypothetical protein -
  BVH74_RS08530 (BVH74_08530) - 1856627..1857154 (-) 528 WP_080049645.1 GspH/FimT family pseudopilin -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62785.02 Da        Isoelectric Point: 5.3240

>NTDB_id=221091 BVH74_RS08520 WP_080049643.1 1853462..1855165(-) (pilB) [Halopseudomonas phragmitis strain S-6-2]
METPVLSGLARRIVQVELLDPQVASKASKQAHQDKIPLITYLVQNKLANPRDLAMVCAEEFGYPYFDLVSLDKESQPPQD
LVSEKLVRQHNVLPLFKRGSRLYLALSDPTNQQALSDIQFHTGLMTDAVIVEDDKLHSAIDKYLDSPTGGLGDMSDADLD
NLDVQAGKDEDKGEATDGADDAPIVRFVNKMLLDAIRMGSSDLHFEPYEKLYRVRFRTDGVLHEMAKPPVQLGVRIAARL
KVMSAMDMAERRKPQDGRIKMKISKTKAIDFRVNTLPTLWGEKVVLRILDSDSAKMGIDSLGYEEDQKQMYLDALAKPQG
MILVTGPTGSGKTVSLYTGLNILNTMERNISTAEDPVEINLEGINQVNVNPKQGLDFAQALRAFLRQDPDIIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARRLCKSCKQPMEVPRDTLLAEGFTE
QQIDAGLTIYGPVGCENCKDGYKGRVGIYEVVKITQAMQRIIMEEGNSIQISDVAQSEGFRDLRQSALLKVQQGVTSLAE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=221091 BVH74_RS08520 WP_080049643.1 1853462..1855165(-) (pilB) [Halopseudomonas phragmitis strain S-6-2]
ATGGAAACCCCTGTGCTCTCCGGGCTGGCCCGGCGTATTGTTCAAGTCGAGCTGCTTGACCCCCAGGTCGCCAGCAAAGC
CAGTAAACAGGCCCACCAGGACAAGATCCCGCTGATCACCTATCTGGTGCAGAACAAGCTGGCCAACCCCCGTGACCTGG
CTATGGTCTGCGCCGAGGAATTCGGCTACCCCTATTTCGACCTTGTCTCGCTGGACAAGGAAAGCCAGCCACCCCAGGAT
CTGGTCAGCGAAAAGCTGGTGCGCCAGCACAATGTACTGCCGCTGTTCAAACGCGGCTCAAGACTTTACCTGGCGCTGAG
CGACCCAACCAATCAGCAGGCGCTGAGCGACATCCAGTTTCATACCGGGCTGATGACCGATGCCGTCATTGTTGAAGACG
ACAAGCTGCACAGCGCCATCGACAAATATCTGGACAGCCCAACCGGTGGGCTCGGCGACATGTCCGATGCCGATCTGGAC
AATCTGGACGTACAGGCCGGTAAGGATGAAGACAAGGGTGAAGCAACAGATGGTGCTGACGATGCGCCGATCGTGCGCTT
CGTCAACAAGATGCTGCTGGATGCCATTCGCATGGGCTCATCGGATTTGCACTTTGAGCCCTATGAAAAGCTCTACCGGG
TGCGCTTTCGTACCGATGGCGTACTGCACGAGATGGCCAAGCCACCGGTACAGCTGGGGGTGCGTATTGCTGCCCGCCTG
AAGGTCATGTCAGCCATGGACATGGCCGAACGGCGCAAACCCCAGGATGGCCGGATCAAGATGAAGATTTCCAAGACCAA
GGCCATCGATTTCCGGGTCAATACCCTGCCCACGCTGTGGGGCGAGAAGGTGGTGCTGCGTATTCTCGACTCGGACAGCG
CCAAGATGGGTATCGACTCGCTGGGCTATGAGGAAGACCAGAAACAGATGTACCTGGACGCCCTGGCCAAACCACAGGGC
ATGATCCTGGTTACCGGCCCAACCGGCTCGGGCAAGACCGTCTCCCTTTATACCGGCCTGAATATTCTCAATACCATGGA
ACGGAATATCTCCACTGCCGAAGATCCGGTGGAAATCAACCTTGAAGGTATCAACCAGGTCAACGTCAATCCCAAGCAGG
GCCTGGACTTTGCCCAGGCCCTGCGCGCCTTTTTGCGTCAGGACCCGGATATCATCATGGTTGGTGAGATCCGCGACCTG
GAAACGGCGGAAATCGCCATCAAGGCAGCCCAGACCGGGCACATGGTAATGTCTACCCTGCACACCAACAGTGCGGCAGA
AACCCTGACCCGTTTGCGCAACATGGGTGTGCCCTCATTCAACATTGCTACCTCTGTCAATCTGATCATCGCCCAGCGTC
TGGCGCGGCGGCTGTGCAAAAGTTGCAAGCAGCCGATGGAGGTGCCACGTGACACATTGCTGGCCGAAGGCTTTACTGAA
CAACAGATAGATGCCGGGCTGACCATTTATGGACCGGTGGGTTGTGAAAATTGCAAGGACGGATACAAGGGCCGTGTCGG
GATTTATGAAGTAGTTAAAATAACGCAGGCCATGCAACGTATTATCATGGAAGAAGGTAATTCCATTCAGATTTCGGATG
TCGCCCAAAGCGAAGGCTTCCGCGACCTGCGCCAGTCAGCCCTGCTGAAAGTACAGCAGGGTGTGACCAGCCTGGCGGAA
GTCAACCGAGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1V0B4G5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

61.404

100

0.617
  pilB Acinetobacter baylyi ADP1

58.938

99.647

0.587
  pilB Legionella pneumophila strain ERS1305867

56.966

100

0.57
  pilF Neisseria gonorrhoeae MS11

50.088

99.647

0.499
  pilB Vibrio cholerae strain A1552

49.823

99.471

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

54.694

86.42

0.473
  pilB Vibrio campbellii strain DS40M4

54.268

86.772

0.471
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.15

94.004

0.377
  pilF Thermus thermophilus HB27

37.128

100

0.374

Multiple sequence alignment