Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   B2J95_RS20995 Genome accession   NZ_CP020089
Coordinates   4269610..4270590 (-) Length   326 a.a.
NCBI ID   WP_029883139.1    Uniprot ID   A0AAE2JRL6
Organism   Enterobacter cloacae strain PIMB10EC27     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4264610..4275590
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B2J95_RS20965 (B2J95_20900) - 4265486..4265983 (+) 498 WP_003860035.1 SprT family zinc-dependent metalloprotease -
  B2J95_RS20970 (B2J95_20905) endA 4266078..4266785 (+) 708 WP_013098661.1 deoxyribonuclease I -
  B2J95_RS20975 (B2J95_20910) rsmE 4266838..4267569 (+) 732 WP_057071845.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  B2J95_RS20980 (B2J95_20915) gshB 4267589..4268536 (+) 948 WP_023620782.1 glutathione synthase -
  B2J95_RS20985 (B2J95_20920) - 4268623..4269183 (+) 561 WP_013098664.1 YqgE/AlgH family protein -
  B2J95_RS20990 (B2J95_20925) ruvX 4269183..4269599 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  B2J95_RS20995 (B2J95_20930) pilT 4269610..4270590 (-) 981 WP_029883139.1 type IV pilus twitching motility protein PilT Machinery gene
  B2J95_RS21000 (B2J95_20935) - 4270608..4271309 (+) 702 WP_020687058.1 YggS family pyridoxal phosphate-dependent enzyme -
  B2J95_RS21005 (B2J95_20940) - 4271331..4271897 (+) 567 WP_013098667.1 YggT family protein -
  B2J95_RS21010 (B2J95_20945) yggU 4271894..4272181 (+) 288 WP_058678490.1 DUF167 family protein YggU -
  B2J95_RS21015 (B2J95_20950) - 4272194..4272787 (+) 594 WP_058678489.1 XTP/dITP diphosphatase -
  B2J95_RS21020 (B2J95_20955) hemW 4272780..4273928 (+) 1149 WP_058678488.1 radical SAM family heme chaperone HemW -
  B2J95_RS21025 (B2J95_20960) - 4273985..4274323 (+) 339 WP_058678487.1 endonuclease domain-containing protein -
  B2J95_RS21030 (B2J95_20965) - 4274406..4275122 (-) 717 WP_014833228.1 DUF2884 domain-containing protein -
  B2J95_RS21035 (B2J95_20970) - 4275179..4275505 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35652.66 Da        Isoelectric Point: 6.3038

>NTDB_id=221013 B2J95_RS20995 WP_029883139.1 4269610..4270590(-) (pilT) [Enterobacter cloacae strain PIMB10EC27]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRVGRLEPAPFPSPDVDALLKTWLNDEQQGAWWASGQVDFAVTLTGNQRLRA
SAFKQMKGNSITLRLLPRACPQLSALGVPRAIPELLSHDSGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FVYQSERCLIQQREIGLHSPSFAEALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=221013 B2J95_RS20995 WP_029883139.1 4269610..4270590(-) (pilT) [Enterobacter cloacae strain PIMB10EC27]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGAGTAGGCCGCCTTGAACCGGCTCCCTTTCCCTCTCCCGATGTGGATGCGTTACTGAAAACGTGGCTCAACG
ATGAGCAGCAGGGGGCATGGTGGGCAAGCGGGCAGGTGGATTTTGCCGTGACACTTACGGGAAACCAGCGCCTGCGCGCA
AGCGCCTTTAAACAGATGAAGGGCAACTCAATCACGCTACGCCTGTTGCCCCGGGCCTGTCCGCAGCTCTCCGCCCTGGG
TGTTCCCCGGGCTATCCCGGAACTCTTATCCCACGACAGTGGGCTGATACTGGTGACGGGGGCAACCGGCAGCGGCAAAT
CTACCACCCTTGCGGCAATGGTGGATTTTCTTAATCACCAGACGGATGGGCATATTCTGACGCTGGAAGATCCGGTGGAG
TTTGTCTATCAGAGCGAACGTTGCCTGATTCAGCAGCGAGAGATCGGCCTGCACAGTCCCTCATTTGCCGAGGCGCTGCG
CGCTGCGTTGCGTGAAGATCCGGATGTCATCCTGCTGGGAGAACTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CCGCTGAAACCGGGCATCTGGTGCTGGCGACGCTGCACACGCGCGGTGCCTCTCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGACCCGGTGCGTAATCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAGACTGCTTCC
CGACCTGCAGGGTGGGCGCGTCGCGCTGTATGAACTGCTGGTGAATACGCCAGCGGCAGCGAACCTTATCCGCGAAGGTA
AAACGTGGCAGTTGCCCGGGATTATTCAAACCGGTCAGCAGGCGGGGATGCAGAACTTTGACCAGAGCCTGGCAGAACGA
CGGGCGCAGGGGCGGTTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Legionella pneumophila strain ERS1305867

49.068

98.773

0.485
  pilT Legionella pneumophila strain Lp02

49.068

98.773

0.485
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

48.438

98.16

0.475
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.168

98.773

0.426
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilB Haemophilus influenzae 86-028NP

33.708

100

0.368
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment