Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BVH73_RS05845 Genome accession   NZ_CP020046
Coordinates   1251722..1252768 (-) Length   348 a.a.
NCBI ID   WP_079416939.1    Uniprot ID   -
Organism   Thiomonas intermedia strain ATCC 15466     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1246722..1257768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVH73_RS05825 mutY 1247818..1248939 (+) 1122 WP_245800432.1 A/G-specific adenine glycosylase -
  BVH73_RS05830 - 1248936..1249811 (+) 876 WP_079416933.1 DUF429 domain-containing protein -
  BVH73_RS05835 pilU 1249824..1250960 (-) 1137 WP_079416935.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BVH73_RS05840 - 1251019..1251657 (-) 639 WP_079416937.1 cyclic nucleotide-binding domain-containing protein -
  BVH73_RS05845 pilT 1251722..1252768 (-) 1047 WP_079416939.1 type IV pilus twitching motility protein PilT Machinery gene
  BVH73_RS05850 - 1252908..1253609 (+) 702 WP_079416941.1 YggS family pyridoxal phosphate-dependent enzyme -
  BVH73_RS05855 - 1253727..1254728 (+) 1002 WP_245800433.1 hypothetical protein -
  BVH73_RS05860 - 1254725..1255012 (+) 288 WP_079416945.1 acylphosphatase -
  BVH73_RS05865 - 1255137..1255694 (+) 558 WP_079416947.1 flavodoxin family protein -
  BVH73_RS05870 - 1255736..1256803 (-) 1068 WP_079420380.1 3-deoxy-7-phosphoheptulonate synthase -
  BVH73_RS05875 - 1256830..1257189 (-) 360 WP_079416949.1 thioredoxin -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38492.16 Da        Isoelectric Point: 6.9395

>NTDB_id=220671 BVH73_RS05845 WP_079416939.1 1251722..1252768(-) (pilT) [Thiomonas intermedia strain ATCC 15466]
MDITQLLAFSVKNDASDLHLSAGLPPMIRVHGDVRRINVEPLDHKAVHAMVYDIMSDSQRKHYEEFLEVDFSFEVPQLAR
FRVNAFNQARGAGAVFRTIPSKVLTLDDLNAPKIFADLALKPRGLVLVTGPTGSGKSTTLAAMINHYNETEYGHILTIED
PIEFVHQSRKALINQREVGPHTLSFSNALRSALREDPDAILVGEMRDLETIRLALTASETGHLVFATLHTSSAPKTIDRI
VDVFPAEEKEMVRAMLSEALIGVISQTLCKKKDGSGRVAAHEIMIGTPAIRNLIRENKIAQMYSMIQTSQSYGMQTLDQN
LADLVKRNVISATEARTKAKQPENFPGA

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=220671 BVH73_RS05845 WP_079416939.1 1251722..1252768(-) (pilT) [Thiomonas intermedia strain ATCC 15466]
TTGGACATTACCCAACTTCTCGCTTTCAGCGTCAAGAACGACGCATCCGATCTGCATCTGTCCGCCGGCCTGCCGCCCAT
GATCCGCGTGCACGGCGACGTGCGGCGCATCAACGTCGAGCCGCTCGATCACAAGGCGGTGCATGCCATGGTGTACGACA
TCATGAGCGATTCGCAGCGCAAGCATTACGAGGAGTTCCTCGAGGTCGATTTCTCCTTCGAAGTGCCGCAGCTGGCGCGT
TTCCGGGTCAATGCCTTCAACCAGGCGCGCGGCGCCGGCGCGGTGTTCCGCACCATTCCGAGCAAGGTGCTCACGCTCGA
CGATCTCAACGCGCCGAAGATCTTCGCCGATCTCGCACTCAAGCCGAGGGGTCTGGTGCTGGTCACCGGGCCCACGGGTT
CGGGCAAGTCGACCACCCTGGCGGCGATGATCAATCACTACAACGAGACCGAGTACGGCCACATTCTCACCATTGAAGAC
CCCATCGAGTTCGTGCACCAGTCCCGCAAGGCGCTGATCAATCAGCGCGAGGTCGGGCCGCACACCCTGAGTTTTTCCAA
CGCCCTGCGCTCGGCGCTGCGCGAAGACCCGGACGCGATTCTGGTGGGCGAAATGCGCGATCTCGAAACCATCCGGCTGG
CGCTGACCGCGTCTGAAACCGGCCACCTGGTGTTCGCCACCCTGCATACCTCTTCCGCGCCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCGGCCGAAGAAAAGGAAATGGTGCGCGCCATGCTGTCGGAAGCCCTGATCGGCGTGATTTCGCAGAC
GCTGTGCAAGAAAAAGGACGGTTCCGGACGGGTGGCCGCGCATGAAATCATGATCGGCACGCCAGCCATCCGCAATCTGA
TCCGCGAAAACAAGATCGCGCAGATGTATTCCATGATCCAGACGAGCCAGTCCTACGGCATGCAGACGCTCGACCAGAAC
CTGGCCGATCTGGTCAAGCGCAACGTGATCTCCGCCACCGAGGCGCGCACCAAGGCCAAGCAGCCCGAGAATTTCCCCGG
AGCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

75.942

99.138

0.753
  pilT Acinetobacter baumannii D1279779

75.942

99.138

0.753
  pilT Acinetobacter baumannii strain A118

75.942

99.138

0.753
  pilT Pseudomonas aeruginosa PAK

75.942

99.138

0.753
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.138

0.747
  pilT Acinetobacter baylyi ADP1

72.754

99.138

0.721
  pilT Legionella pneumophila strain ERS1305867

70.501

97.414

0.687
  pilT Legionella pneumophila strain Lp02

70.501

97.414

0.687
  pilT Neisseria meningitidis 8013

68.406

99.138

0.678
  pilT Neisseria gonorrhoeae MS11

68.116

99.138

0.675
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.976

97.126

0.641
  pilT Vibrio cholerae strain A1552

65.976

97.126

0.641
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

99.138

0.514
  pilU Acinetobacter baylyi ADP1

41.329

99.425

0.411
  pilU Vibrio cholerae strain A1552

41.791

96.264

0.402
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.264

0.397

Multiple sequence alignment