Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BVH73_RS05835 Genome accession   NZ_CP020046
Coordinates   1249824..1250960 (-) Length   378 a.a.
NCBI ID   WP_079416935.1    Uniprot ID   -
Organism   Thiomonas intermedia strain ATCC 15466     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1244824..1255960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVH73_RS05815 mutM 1244875..1245765 (+) 891 WP_079416927.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  BVH73_RS05820 - 1245827..1247752 (+) 1926 WP_079416929.1 dynamin family protein -
  BVH73_RS05825 mutY 1247818..1248939 (+) 1122 WP_245800432.1 A/G-specific adenine glycosylase -
  BVH73_RS05830 - 1248936..1249811 (+) 876 WP_079416933.1 DUF429 domain-containing protein -
  BVH73_RS05835 pilU 1249824..1250960 (-) 1137 WP_079416935.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BVH73_RS05840 - 1251019..1251657 (-) 639 WP_079416937.1 cyclic nucleotide-binding domain-containing protein -
  BVH73_RS05845 pilT 1251722..1252768 (-) 1047 WP_079416939.1 type IV pilus twitching motility protein PilT Machinery gene
  BVH73_RS05850 - 1252908..1253609 (+) 702 WP_079416941.1 YggS family pyridoxal phosphate-dependent enzyme -
  BVH73_RS05855 - 1253727..1254728 (+) 1002 WP_245800433.1 hypothetical protein -
  BVH73_RS05860 - 1254725..1255012 (+) 288 WP_079416945.1 acylphosphatase -
  BVH73_RS05865 - 1255137..1255694 (+) 558 WP_079416947.1 flavodoxin family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42000.21 Da        Isoelectric Point: 6.5925

>NTDB_id=220670 BVH73_RS05835 WP_079416935.1 1249824..1250960(-) (pilU) [Thiomonas intermedia strain ATCC 15466]
MDRDQASKFINDLLKLMISRGGSDLFLTADFPPAIKVDGAVSKLSSQALSSQHTLALARSIMNDKQSAEFERTKECNFAI
SPAGLTRFRCNAFIQQGKVGIVLRQIPQELPSIDGLELPPVLKTLAMHKRGLIIFVGATGSGKSTSMAAMLDFRNENSYG
HIITVEDPIEFVHPHKNCIVTQREVGLDTDTWEAALKNTLRQAPDVILMGEIRDRETMEHAVVFSETGHLVLATLHANNA
NQALDRIINFFPEERRSQLLMDLSLNLRALVSQRLVPLQTGKGRVAAVEILINSPLIADQIFEGKLTEIKDVMRRSRELG
MQTFDQSLFDLFDAEKIGYEDALRNADSVNDLRLRIKLDSKRARNADIGSDTGHLAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=220670 BVH73_RS05835 WP_079416935.1 1249824..1250960(-) (pilU) [Thiomonas intermedia strain ATCC 15466]
ATGGATCGTGATCAGGCTTCCAAATTCATCAACGATCTGCTCAAGCTGATGATCAGCCGCGGCGGCTCGGACCTGTTCCT
CACCGCCGATTTTCCGCCGGCGATCAAGGTCGATGGGGCCGTTTCCAAGCTGTCGTCGCAGGCGCTCAGCAGCCAGCACA
CGCTGGCGCTGGCGCGCTCGATCATGAACGACAAGCAGTCGGCCGAATTCGAGCGCACCAAAGAGTGCAATTTCGCCATC
TCGCCTGCCGGGCTCACCCGCTTTCGCTGCAATGCCTTCATCCAGCAGGGCAAAGTGGGCATCGTGCTGCGGCAGATCCC
CCAGGAGCTGCCTTCCATCGACGGCCTGGAATTGCCGCCGGTGCTCAAGACGCTGGCCATGCACAAGCGCGGACTCATCA
TCTTTGTCGGCGCCACGGGCTCGGGCAAATCGACCTCGATGGCCGCGATGCTCGATTTCCGCAACGAAAATTCCTACGGC
CACATCATCACGGTGGAAGACCCGATCGAGTTCGTGCATCCGCACAAGAACTGCATCGTGACCCAGCGCGAGGTGGGGCT
CGATACCGACACCTGGGAGGCGGCGCTCAAGAACACCCTGCGGCAGGCGCCCGATGTCATTCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGAGCATGCCGTGGTGTTCTCCGAGACCGGCCACCTGGTGCTGGCCACGCTGCACGCCAACAATGCC
AACCAGGCGCTCGATCGCATCATCAATTTCTTCCCTGAAGAGCGGCGATCGCAACTCCTGATGGACTTGTCGCTCAATCT
GCGCGCGCTGGTGTCGCAGCGGCTGGTGCCGCTGCAGACGGGCAAGGGGCGCGTGGCTGCCGTCGAGATCCTGATCAATT
CGCCCTTGATTGCCGATCAGATCTTCGAAGGCAAGCTCACCGAGATCAAGGACGTGATGCGGCGCTCGCGCGAACTGGGC
ATGCAGACCTTCGACCAGTCGTTGTTCGACCTGTTCGATGCCGAGAAAATCGGCTACGAAGATGCGCTGCGCAACGCCGA
TTCGGTCAACGATCTGCGTCTGCGCATCAAGCTCGACAGCAAACGGGCCCGCAACGCCGATATCGGCAGCGACACCGGGC
ATCTGGCCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.605

92.328

0.569
  pilU Acinetobacter baylyi ADP1

57.542

94.709

0.545
  pilU Vibrio cholerae strain A1552

54.144

95.767

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.51

89.153

0.397
  pilT Legionella pneumophila strain Lp02

43.544

88.095

0.384
  pilT Legionella pneumophila strain ERS1305867

43.544

88.095

0.384
  pilT Pseudomonas aeruginosa PAK

43.027

89.153

0.384
  pilT Pseudomonas stutzeri DSM 10701

42.73

89.153

0.381
  pilT Acinetobacter baumannii D1279779

44.201

84.392

0.373
  pilT Acinetobacter baumannii strain A118

44.201

84.392

0.373
  pilT Acinetobacter nosocomialis M2

44.201

84.392

0.373
  pilT Acinetobacter baylyi ADP1

43.344

85.45

0.37

Multiple sequence alignment