Detailed information NTG0220196

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: CXX14_RS10775
• Genome accession: NZ_CP025849
• Coordinates: 2261145..2262125 (+)
• Length: 326 a.a.
• NCBI ID: WP_001055620.1
• Uniprot ID: -
• Organism: Escherichia coli strain 600468
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2256145..2267125

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CXX14_RS10745	yggM	2256660..2257667 (+)	1008	WP_000745217.1	DUF1202 family protein	-
CXX14_RS10750	hemW	2257822..2258958 (-)	1137	WP_000239939.1	radical SAM family heme chaperone HemW	-
CXX14_RS10755	rdgB	2258951..2259544 (-)	594	WP_001174747.1	XTP/dITP diphosphatase	-
CXX14_RS10760	yggU	2259552..2259842 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
CXX14_RS10765	yggT	2259839..2260405 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
CXX14_RS10770	yggS	2260423..2261127 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
CXX14_RS10775	pilT	2261145..2262125 (+)	981	WP_001055620.1	type IV pilus twitching motility protein PilT	Machinery gene
CXX14_RS10780	ruvX	2262301..2262717 (-)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
CXX14_RS10785	yqgE	2262717..2263280 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
CXX14_RS10790	gshB	2263389..2264339 (-)	951	WP_000593273.1	glutathione synthase	-
CXX14_RS10795	rsmE	2264352..2265083 (-)	732	WP_001222508.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
CXX14_RS10800	endA	2265163..2265870 (-)	708	WP_000286500.1	deoxyribonuclease I	-
CXX14_RS10805	yggI	2265965..2266462 (-)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35903.08 Da
• Isoelectric Point: 5.5860

>NTDB_id=220196 CXX14_RS10775 WP_001055620.1 2261145..2262125(+) (pilT) [Escherichia coli strain 600468]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=220196 CXX14_RS10775 WP_001055620.1 2261145..2262125(+) (pilT) [Escherichia coli strain 600468]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	44.954	100	0.451
pilT	Legionella pneumophila strain Lp02	44.954	100	0.451
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	40.694	97.239	0.396
pilU	Pseudomonas stutzeri DSM 10701	37.879	100	0.383
pilU	Acinetobacter baylyi ADP1	37.346	99.387	0.371
pilB	Legionella pneumophila strain ERS1305867	31.152	100	0.365