Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CPS_RS16385 Genome accession   NC_003910
Coordinates   3806379..3807422 (-) Length   347 a.a.
NCBI ID   WP_011044416.1    Uniprot ID   Q47XZ0
Organism   Colwellia psychrerythraea 34H     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3801379..3812422
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CPS_RS16360 (CPS_3657) cobU 3801434..3801994 (-) 561 WP_011044411.1 bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase -
  CPS_RS16365 (CPS_3658) - 3801991..3802878 (-) 888 WP_011044412.1 adenosylcobinamide-GDP ribazoletransferase -
  CPS_RS16370 (CPS_3659) cobT 3802875..3804053 (-) 1179 WP_011044413.1 nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase -
  CPS_RS16375 (CPS_3660) - 3804500..3805048 (+) 549 WP_011044414.1 hypothetical protein -
  CPS_RS16380 (CPS_3661) pilU 3805205..3806332 (-) 1128 WP_011044415.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CPS_RS16385 (CPS_3662) pilT 3806379..3807422 (-) 1044 WP_011044416.1 type IV pilus twitching motility protein PilT Machinery gene
  CPS_RS16390 (CPS_3663) - 3807538..3808257 (+) 720 WP_011044417.1 YggS family pyridoxal phosphate-dependent enzyme -
  CPS_RS16395 (CPS_3664) proC 3808284..3809105 (+) 822 WP_011044418.1 pyrroline-5-carboxylate reductase -
  CPS_RS16400 (CPS_3665) - 3809166..3809711 (+) 546 WP_011044419.1 YggT family protein -
  CPS_RS16405 (CPS_3666) - 3809798..3810238 (+) 441 WP_011044420.1 DUF4426 domain-containing protein -
  CPS_RS16410 (CPS_3667) rdgB 3810419..3811039 (+) 621 WP_198560075.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  CPS_RS16415 (CPS_3668) hemW 3811057..3812223 (+) 1167 WP_011044422.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38627.19 Da        Isoelectric Point: 5.7852

>NTDB_id=22015 CPS_RS16385 WP_011044416.1 3806379..3807422(-) (pilT) [Colwellia psychrerythraea 34H]
MDITELLAFSVEHNASDLHLSTGTPPAIRVDGDVRKLNIPAFDGKDVNALVYDIMNDRQRKEYEENLEVDFSFEVPNLAR
FRVNAFNQNRGPAAVFRTIPSKILSLDDLGCPEIFREISDTPRGLVLVTGPTGSGKSTTLAAMVDYINDHKHDHILTIED
PIEFVHENKKCLINQREVHRDTLSFSAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKSMVRSMLSESLRAVISQCLIKRVGGGRVAAHEIMIGVPAIRNLIREDKIAQMYSAIQTGMQHGMQTMDQCL
QGLVNRGMITKQDAMEKAVDKNQFSGF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=22015 CPS_RS16385 WP_011044416.1 3806379..3807422(-) (pilT) [Colwellia psychrerythraea 34H]
ATGGATATTACCGAACTACTCGCATTTAGTGTGGAGCATAACGCATCAGATTTACATTTATCAACAGGTACACCGCCCGC
TATTCGTGTTGATGGTGACGTACGTAAACTGAATATTCCAGCCTTTGATGGCAAAGATGTTAATGCGTTAGTTTATGACA
TTATGAATGATCGTCAACGTAAAGAATATGAAGAAAACTTGGAAGTCGATTTCTCATTTGAAGTACCAAACTTAGCACGT
TTCAGGGTTAATGCTTTTAATCAAAATAGAGGCCCCGCAGCCGTATTTCGTACAATCCCGAGTAAAATCCTTTCACTTGA
TGATTTAGGGTGTCCTGAAATATTTCGTGAAATATCAGATACACCACGTGGCTTGGTGTTAGTTACTGGGCCTACCGGCT
CAGGAAAATCAACCACGTTAGCTGCGATGGTTGATTATATTAATGATCATAAACACGATCATATTCTCACCATTGAAGAT
CCAATAGAATTTGTACATGAAAATAAAAAGTGTCTTATTAATCAGCGTGAAGTTCACCGCGATACGTTAAGCTTTTCAGC
TGCATTACGTTCAGCCTTGCGTGAAGATCCAGATGTGATCTTAGTCGGTGAAATGCGAGATTTAGAAACGATTCGCTTAG
CTATGACAGCAGCAGAAACAGGCCATTTAGTCTTTGGCACCTTGCATACAACCTCAGCACCAAAAACTATTGACCGTATT
ATTGATGTATTTCCAGGCGAAGAAAAATCTATGGTTCGCTCAATGCTGTCAGAGTCACTTCGCGCGGTAATTTCACAATG
CCTAATTAAAAGAGTCGGTGGTGGTCGTGTTGCTGCTCATGAAATTATGATTGGGGTTCCCGCTATTCGAAACCTTATTC
GTGAAGATAAAATTGCCCAAATGTACTCAGCGATTCAAACAGGTATGCAACATGGCATGCAGACTATGGATCAATGTCTA
CAGGGCCTTGTTAACAGAGGCATGATTACTAAACAAGATGCGATGGAAAAAGCAGTTGATAAAAATCAATTTTCAGGCTT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q47XZ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

77.616

99.135

0.769
  pilT Acinetobacter nosocomialis M2

76.453

99.135

0.758
  pilT Acinetobacter baumannii D1279779

76.453

99.135

0.758
  pilT Acinetobacter baumannii strain A118

76.453

99.135

0.758
  pilT Pseudomonas aeruginosa PAK

77.515

97.406

0.755
  pilT Acinetobacter baylyi ADP1

75.872

99.135

0.752
  pilT Legionella pneumophila strain Lp02

73.837

99.135

0.732
  pilT Legionella pneumophila strain ERS1305867

73.837

99.135

0.732
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

73.294

97.118

0.712
  pilT Vibrio cholerae strain A1552

73.294

97.118

0.712
  pilT Neisseria meningitidis 8013

67.341

99.712

0.671
  pilT Neisseria gonorrhoeae MS11

67.052

99.712

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.89

99.712

0.527
  pilU Vibrio cholerae strain A1552

42.274

98.847

0.418
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.525

98.847

0.401

Multiple sequence alignment