Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CPS_RS16380 Genome accession   NC_003910
Coordinates   3805205..3806332 (-) Length   375 a.a.
NCBI ID   WP_011044415.1    Uniprot ID   Q47XZ1
Organism   Colwellia psychrerythraea 34H     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3800205..3811332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CPS_RS16355 (CPS_3656) - 3800583..3801416 (-) 834 WP_011044410.1 histidine phosphatase family protein -
  CPS_RS16360 (CPS_3657) cobU 3801434..3801994 (-) 561 WP_011044411.1 bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase -
  CPS_RS16365 (CPS_3658) - 3801991..3802878 (-) 888 WP_011044412.1 adenosylcobinamide-GDP ribazoletransferase -
  CPS_RS16370 (CPS_3659) cobT 3802875..3804053 (-) 1179 WP_011044413.1 nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase -
  CPS_RS16375 (CPS_3660) - 3804500..3805048 (+) 549 WP_011044414.1 hypothetical protein -
  CPS_RS16380 (CPS_3661) pilU 3805205..3806332 (-) 1128 WP_011044415.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CPS_RS16385 (CPS_3662) pilT 3806379..3807422 (-) 1044 WP_011044416.1 type IV pilus twitching motility protein PilT Machinery gene
  CPS_RS16390 (CPS_3663) - 3807538..3808257 (+) 720 WP_011044417.1 YggS family pyridoxal phosphate-dependent enzyme -
  CPS_RS16395 (CPS_3664) proC 3808284..3809105 (+) 822 WP_011044418.1 pyrroline-5-carboxylate reductase -
  CPS_RS16400 (CPS_3665) - 3809166..3809711 (+) 546 WP_011044419.1 YggT family protein -
  CPS_RS16405 (CPS_3666) - 3809798..3810238 (+) 441 WP_011044420.1 DUF4426 domain-containing protein -
  CPS_RS16410 (CPS_3667) rdgB 3810419..3811039 (+) 621 WP_198560075.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41331.12 Da        Isoelectric Point: 5.0148

>NTDB_id=22014 CPS_RS16380 WP_011044415.1 3805205..3806332(-) (pilU) [Colwellia psychrerythraea 34H]
MNLHELLERMVKQDASDMFVTAKLPVSAKINGDLQPIDDVVLTAEQSLALVYDSMNEKQKNQFDTEKECNFAIQIDDIGR
FRISAFWQRDMAGMVVRRIVTDIPDVDDLGLPPILKDVVMSKRGLVLFVGGTGTGKSTSMASLIGYRNRNSCGHILTIED
PVEFVHEHGKSMVTQREVGLDTVSFDAALQSSLRQAPDVILIGEIRNQEIMEHALSFAETGHLCIATLHANNANQAIDRI
MHLVPADQHGKLLFDLALNLRGIIAQQLVPTKDGHSRVAAIEVLLNSPYISELIKKGDIGGIKEVMEKSNELGMQTFDQA
LFNHYQQGMITYADALHHADSPNDLRLMIKLRSNEQGGTGGLSGVTLDGFDSTDN

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=22014 CPS_RS16380 WP_011044415.1 3805205..3806332(-) (pilU) [Colwellia psychrerythraea 34H]
ATGAATCTTCATGAATTATTAGAAAGGATGGTAAAGCAAGACGCCTCAGATATGTTCGTTACGGCAAAATTACCAGTGAG
CGCTAAAATAAATGGTGATTTACAGCCTATAGATGACGTGGTACTGACTGCTGAACAGTCATTAGCTTTAGTTTATGACT
CCATGAATGAGAAGCAAAAAAATCAATTTGATACTGAAAAAGAATGTAATTTTGCCATTCAAATTGATGACATCGGTCGT
TTTCGTATTTCTGCTTTTTGGCAACGAGATATGGCGGGCATGGTTGTTCGTCGTATTGTTACAGATATTCCTGATGTTGA
TGATTTAGGCTTACCTCCGATTTTAAAAGATGTGGTTATGTCTAAACGTGGTTTAGTGCTTTTTGTGGGTGGTACCGGTA
CTGGTAAATCGACATCGATGGCTTCATTGATAGGTTATCGTAACCGTAACTCTTGTGGGCATATTCTTACTATTGAAGAC
CCAGTGGAGTTTGTTCATGAGCACGGAAAGAGTATGGTCACTCAGCGTGAAGTTGGCTTAGATACCGTATCCTTTGATGC
GGCACTGCAAAGCTCATTACGCCAAGCACCTGATGTCATTTTAATTGGTGAGATTCGAAACCAAGAGATAATGGAGCATG
CGCTTAGTTTTGCAGAAACGGGGCATTTATGTATAGCTACCTTGCATGCAAACAATGCTAACCAAGCTATTGACCGGATT
ATGCACTTAGTGCCAGCGGACCAGCATGGAAAGTTGTTGTTTGATTTAGCGTTAAATCTGCGCGGTATTATTGCCCAGCA
ACTGGTACCGACAAAAGATGGTCATAGCCGAGTTGCCGCCATCGAAGTATTGCTTAATTCTCCTTATATTAGTGAACTCA
TTAAAAAAGGTGACATTGGTGGTATTAAAGAGGTGATGGAAAAATCAAATGAATTAGGTATGCAAACGTTTGACCAAGCA
TTATTCAACCATTACCAACAGGGTATGATTACTTATGCGGATGCATTACATCATGCGGACTCGCCGAATGATTTACGTTT
AATGATTAAATTGCGCAGTAATGAGCAGGGTGGTACAGGCGGCTTATCAGGTGTCACATTAGATGGCTTCGACTCTACAG
ATAACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q47XZ1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.909

99.467

0.576
  pilU Acinetobacter baylyi ADP1

58.239

93.867

0.547
  pilU Vibrio cholerae strain A1552

52.174

98.133

0.512
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.771

96.8

0.395
  pilT Pseudomonas aeruginosa PAK

40

92

0.368

Multiple sequence alignment