Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BUM88_RS04245 Genome accession   NZ_CP020000
Coordinates   874107..875144 (-) Length   345 a.a.
NCBI ID   WP_003653239.1    Uniprot ID   A0A0B2UAY6
Organism   Acinetobacter calcoaceticus strain CA16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 869107..880144
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BUM88_RS04210 (BUM88_04210) - 869899..870354 (+) 456 WP_017388663.1 bacteriohemerythrin -
  BUM88_RS04220 (BUM88_04220) - 870506..871573 (+) 1068 WP_155120859.1 hypothetical protein -
  BUM88_RS04225 (BUM88_04225) - 871573..871884 (+) 312 WP_080026441.1 RnfH family protein -
  BUM88_RS04230 (BUM88_04230) - 871952..872350 (-) 399 WP_003653245.1 outer membrane protein assembly factor BamE -
  BUM88_RS04235 (BUM88_04235) fur 872462..872899 (+) 438 WP_003653242.1 ferric iron uptake transcriptional regulator -
  BUM88_RS04240 (BUM88_04240) pilU 872969..874087 (-) 1119 WP_003653241.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BUM88_RS04245 (BUM88_04245) pilT 874107..875144 (-) 1038 WP_003653239.1 type IV pilus twitching motility protein PilT Machinery gene
  BUM88_RS04250 (BUM88_04250) - 875271..875963 (+) 693 WP_080026442.1 YggS family pyridoxal phosphate-dependent enzyme -
  BUM88_RS04255 (BUM88_04255) - 876164..879760 (-) 3597 WP_080026443.1 AAA family ATPase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38348.92 Da        Isoelectric Point: 6.7269

>NTDB_id=219723 BUM88_RS04245 WP_003653239.1 874107..875144(-) (pilT) [Acinetobacter calcoaceticus strain CA16]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALDHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMMDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSSIQTGANHGMTTLDQSL
KGLVSRGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=219723 BUM88_RS04245 WP_003653239.1 874107..875144(-) (pilT) [Acinetobacter calcoaceticus strain CA16]
ATGGATATTACAGAGCTACTCGCTTTCTCTGTGAAAAATGGCGCGTCCGATTTACACTTATCTGCTGGCATGCCACCAAT
GATTCGCGTGGATGGTGAAGTCCGTCGCATTAACTTACCAGCTCTAGATCATAAAGATGTTCACCGTCTGGTCTATGACA
TCATGAATGATAAACAACGTCGTGACTATGAAGAAAAGCTCGAAACAGACTTTTCTTTTGAAGTTCCCAATGTTGCGCGT
TTTCGTGTCAATGCATTTAACCAGAACCGCGGTGCCGGTGCAGTGTTTCGTACCATTCCATCAAAAGTACTCACCATGGA
AGATTTAGGCTTAGGTCAAATCTTTAAAGATATTTGTGATTACCCACGTGGCATTGTGTTAGTTACAGGACCAACAGGTT
CTGGTAAATCAACCACCCTTGCAGCGATGATGGACTATATCAACGAAAATCGTTATGACCACATTTTAACGGTTGAAGAC
CCAATCGAATTTGTACACCAGTCGAAGAAATGTTTGATTAACCAACGTGAAGTGCATCGTGATACGCATGGCTTTAATGA
AGCACTACGTTCGGCACTGCGTGAAGACCCCGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAAACCATTCGTTTAG
CACTTACCGCTGCTGAAACTGGTCACTTGGTGTTTGGTACGCTCCATACGACTTCTGCTGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTTCGTGCCATGTTGTCAGAATCTTTACAGGCTGTTATTTCGCAAAC
CCTTCTTAAAAAGAATGGTGGTGGACGTGTCGCAGCACATGAAATCATGATTGGTATTCCTGCCATTCGTAACTTAATTC
GTGAAAATAAAGTCGCACAAATGTATTCATCAATTCAGACTGGTGCAAATCATGGCATGACGACACTCGACCAAAGCTTA
AAAGGTTTGGTATCTCGTGGTGTGATTAGCCCACAAACTGCACGTACTGCTGCAAAACAGCCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B2UAY6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

99.13

100

0.991
  pilT Acinetobacter baumannii D1279779

98.841

100

0.988
  pilT Acinetobacter baumannii strain A118

98.841

100

0.988
  pilT Acinetobacter baylyi ADP1

88.953

99.71

0.887
  pilT Pseudomonas stutzeri DSM 10701

84.012

99.71

0.838
  pilT Pseudomonas aeruginosa PAK

83.14

99.71

0.829
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.429

97.391

0.696
  pilT Vibrio cholerae strain A1552

71.429

97.391

0.696
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

39.017

100

0.391

Multiple sequence alignment