Detailed information NTG0219722

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: BUM88_RS04240
• Genome accession: NZ_CP020000
• Coordinates: 872969..874087 (-)
• Length: 372 a.a.
• NCBI ID: WP_003653241.1
• Uniprot ID: -
• Organism: Acinetobacter calcoaceticus strain CA16
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 867969..879087

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BUM88_RS04205 (BUM88_04205)	-	868799..869647 (+)	849	WP_003653249.1	class II glutamine amidotransferase	-
BUM88_RS04210 (BUM88_04210)	-	869899..870354 (+)	456	WP_017388663.1	bacteriohemerythrin	-
BUM88_RS04220 (BUM88_04220)	-	870506..871573 (+)	1068	WP_155120859.1	hypothetical protein	-
BUM88_RS04225 (BUM88_04225)	-	871573..871884 (+)	312	WP_080026441.1	RnfH family protein	-
BUM88_RS04230 (BUM88_04230)	-	871952..872350 (-)	399	WP_003653245.1	outer membrane protein assembly factor BamE	-
BUM88_RS04235 (BUM88_04235)	fur	872462..872899 (+)	438	WP_003653242.1	ferric iron uptake transcriptional regulator	-
BUM88_RS04240 (BUM88_04240)	pilU	872969..874087 (-)	1119	WP_003653241.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
BUM88_RS04245 (BUM88_04245)	pilT	874107..875144 (-)	1038	WP_003653239.1	type IV pilus twitching motility protein PilT	Machinery gene
BUM88_RS04250 (BUM88_04250)	-	875271..875963 (+)	693	WP_080026442.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41889.07 Da
• Isoelectric Point: 6.6400

>NTDB_id=219722 BUM88_RS04240 WP_003653241.1 872969..874087(-) (pilU) [Acinetobacter calcoaceticus strain CA16]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGDVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNTKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLAEEGPDQISGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=219722 BUM88_RS04240 WP_003653241.1 872969..874087(-) (pilU) [Acinetobacter calcoaceticus strain CA16]
ATGGATTTTAATGACCTACTCAACCTCATGGTTGAAAAAAAATCATCCGATCTTTTTATTACAGATGGTGTTGCGCCATC
TATGAAAATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGGGATGTTATTGGTCAACTATTACATTCCA
TCATGAGCGAAAAGCAGCGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACGGCC
CGCTTTCGTGTGAGCGCTTTTCAGCAGCGTGACATGCCAGGCATGGTCTTACGCCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAGTTACCGCCCGTTCTTAAAGATTTATCAATGACTAAACGCGGCATCATTATTTTTGTTGGTGCAACAG
GTACTGGTAAATCGACGTCTTTGGCTTCAATGATCAGCTATCGCAATCACAATACTAAAGGTCATATTATTACGATTGAA
GACCCAATCGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAACGTGAAGTTGGGATCGATACAGACTCATTTGA
AATTGCCTTAAAGAATACTTTGCGACAAGCACCAGATGTCATTTTAATTGGTGAGATTCGATCTCGTGAAGTTATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCCACAATGCACGCTAACAACGCCAACCAAGCGCTCGATCGT
ATTATTCACTTCTTTGAAAGTGACCGTCACAGTCAGCTTTACATGGACTTATCGCTTAACTTAAAGGCGATGATTGCGCA
GCAACTTATTCCAACACCAGATGGCAATTCACGTCGTGCAGCCATTGAGATTTTGATTAACTCGCCATTAATTTCAGACT
ACATTCGTAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAGCGTTCGCGTGAACTCGGTATGCAAACCTTTGACCAA
GCGTTGTTTGATCTCTATAAAGCAGGCCAAATTACCTATAAAGATGCATTAAAACATGCCGACTCACCGAACGATTTGCG
TTTGACCATTAAGCTTGCCGAAGAAGGACCTGATCAAATTTCAGGCACAAATCAGCATTTAACCTTCGACCGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.397	100	0.766
pilU	Pseudomonas stutzeri DSM 10701	67.655	99.731	0.675
pilU	Vibrio cholerae strain A1552	54.324	99.462	0.54
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39
pilT	Legionella pneumophila strain Lp02	39.645	90.86	0.36
pilT	Legionella pneumophila strain ERS1305867	39.645	90.86	0.36