Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   B2D37_RS16585 Genome accession   NZ_CP019947
Coordinates   3660452..3661486 (-) Length   344 a.a.
NCBI ID   WP_079203726.1    Uniprot ID   -
Organism   Pseudomonas sp. CC6-YY-74     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3655452..3666486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B2D37_RS16560 pyrR 3655738..3656244 (+) 507 WP_079203721.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  B2D37_RS16565 - 3656255..3657265 (+) 1011 WP_079203722.1 aspartate carbamoyltransferase catalytic subunit -
  B2D37_RS16570 - 3657265..3658536 (+) 1272 WP_079203723.1 dihydroorotase -
  B2D37_RS16575 - 3658689..3659090 (-) 402 WP_079203724.1 TM2 domain-containing protein -
  B2D37_RS16580 pilU 3659196..3660341 (-) 1146 WP_079203725.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  B2D37_RS16585 pilT 3660452..3661486 (-) 1035 WP_079203726.1 type IV pilus twitching motility protein PilT Machinery gene
  B2D37_RS16590 - 3661541..3662221 (+) 681 WP_079203727.1 YggS family pyridoxal phosphate-dependent enzyme -
  B2D37_RS16595 proC 3662261..3663094 (+) 834 WP_079203728.1 pyrroline-5-carboxylate reductase -
  B2D37_RS16600 - 3663137..3663727 (+) 591 WP_079203729.1 YggT family protein -
  B2D37_RS16605 - 3663727..3664023 (+) 297 WP_079203730.1 DUF167 family protein -
  B2D37_RS16610 - 3664382..3666364 (+) 1983 WP_079203731.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37962.70 Da        Isoelectric Point: 6.7750

>NTDB_id=219094 B2D37_RS16585 WP_079203726.1 3660452..3661486(-) (pilT) [Pseudomonas sp. CC6-YY-74]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNSTKYQHILTIED
PIEFVHESKKCLINQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGASLGMQTLDACL
KTLVAKGLVTRENAREKSKTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=219094 B2D37_RS16585 WP_079203726.1 3660452..3661486(-) (pilT) [Pseudomonas sp. CC6-YY-74]
ATGGATATCACTGAGCTGCTTGCCTTCAGCGCCAAGCAAGGCGCGTCGGACCTGCATCTCTCTGCTGGTCTGCCGCCGAT
GATTCGGGTTGACGGCGATGTGCGCCGGATCAATTTGCCGGCCCTGGATCACAAGCAGGTGCATGCACTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAGGAGTTCCTCGAGACCGACTTCTCCTTCGAAGTGCCTGGTGTCGCGCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGTGGCGCCGGTGCGGTGTTCCGCACCATTCCGTCCAAGGTGCTGACCATGGA
AGACCTCGGCATGGGCGAGGTGTTTCGCAAGATCACCGACGTGCCACGGGGCCTGGTGCTGGTCACCGGGCCCACGGGTT
CGGGCAAGTCGACCACCCTGGCGGCGATGCTCGATTACCTCAATAGCACCAAGTACCAGCACATCCTGACCATCGAAGAC
CCGATCGAGTTCGTTCACGAGTCGAAGAAGTGCCTGATCAACCAGCGTGAAGTGCACCGCGACACCCTGGGCTTCTCCGA
GGCCCTGCGCTCGGCACTGCGCGAAGACCCGGACATCATTCTGGTCGGCGAGATGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACTGGCCACCTGGTATTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGATCGGGTG
GTCGATGTATTCCCTGCCGAAGAAAAATCCATGGTTCGCTCGATGCTCTCCGAATCCTTGCAGGCGGTGATCTCGCAGAC
CCTGCTGAAGAAAGTCGGCGGCGGTCGGGTGGCGGCCCATGAAATCATGATTGGCACCCCGGCGATTCGTAACCTGATCC
GTGAGGACAAGGTGGCGCAGATGTACTCGTCGATCCAGACCGGCGCTTCGCTGGGCATGCAGACCCTGGATGCCTGCCTG
AAAACCCTGGTAGCCAAGGGCCTGGTCACCCGCGAAAATGCGCGGGAAAAATCCAAGACCCCGGATAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

95.058

100

0.951
  pilT Pseudomonas aeruginosa PAK

93.314

100

0.933
  pilT Acinetobacter baumannii D1279779

84.012

100

0.84
  pilT Acinetobacter baumannii strain A118

84.012

100

0.84
  pilT Acinetobacter nosocomialis M2

83.721

100

0.837
  pilT Acinetobacter baylyi ADP1

82.849

100

0.828
  pilT Legionella pneumophila strain Lp02

74.702

97.674

0.73
  pilT Legionella pneumophila strain ERS1305867

74.702

97.674

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.03

97.965

0.686
  pilT Vibrio cholerae strain A1552

70.03

97.965

0.686
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

100

0.532
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Vibrio cholerae strain A1552

39.71

100

0.398
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39

Multiple sequence alignment