Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   B2D37_RS16580 Genome accession   NZ_CP019947
Coordinates   3659196..3660341 (-) Length   381 a.a.
NCBI ID   WP_079203725.1    Uniprot ID   -
Organism   Pseudomonas sp. CC6-YY-74     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3654196..3665341
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B2D37_RS16550 - 3654598..3655167 (+) 570 WP_079203719.1 YqgE/AlgH family protein -
  B2D37_RS16555 ruvX 3655167..3655604 (+) 438 WP_079203720.1 Holliday junction resolvase RuvX -
  B2D37_RS16560 pyrR 3655738..3656244 (+) 507 WP_079203721.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  B2D37_RS16565 - 3656255..3657265 (+) 1011 WP_079203722.1 aspartate carbamoyltransferase catalytic subunit -
  B2D37_RS16570 - 3657265..3658536 (+) 1272 WP_079203723.1 dihydroorotase -
  B2D37_RS16575 - 3658689..3659090 (-) 402 WP_079203724.1 TM2 domain-containing protein -
  B2D37_RS16580 pilU 3659196..3660341 (-) 1146 WP_079203725.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  B2D37_RS16585 pilT 3660452..3661486 (-) 1035 WP_079203726.1 type IV pilus twitching motility protein PilT Machinery gene
  B2D37_RS16590 - 3661541..3662221 (+) 681 WP_079203727.1 YggS family pyridoxal phosphate-dependent enzyme -
  B2D37_RS16595 proC 3662261..3663094 (+) 834 WP_079203728.1 pyrroline-5-carboxylate reductase -
  B2D37_RS16600 - 3663137..3663727 (+) 591 WP_079203729.1 YggT family protein -
  B2D37_RS16605 - 3663727..3664023 (+) 297 WP_079203730.1 DUF167 family protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42425.63 Da        Isoelectric Point: 6.7254

>NTDB_id=219093 B2D37_RS16580 WP_079203725.1 3659196..3660341(-) (pilU) [Pseudomonas sp. CC6-YY-74]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVTKNPMSPEQTRETVHSVMNEQQRRDFAESHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTLDDLKLPEILKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQNCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQNQVWMDLSLNLKAIVAQQLIPTPDGKGRRAVIEVLINTPLAADLIRKGDVHELKNLMKRSTELGMQTFDQA
LYSLYSQGEITYEDALLYADSANDLRLLIKLGSEADGDHLAHLSDGLSLEVAPDDPGRRFR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=219093 B2D37_RS16580 WP_079203725.1 3659196..3660341(-) (pilU) [Pseudomonas sp. CC6-YY-74]
ATGGAATTCGAAAAACTGCTGCGCCTGATGGTAGAAAAAGGCGGCTCCGACCTGTTTATCACTGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTGACCAAGAACCCCATGTCGCCAGAGCAAACCCGGGAAACCGTGCACTCGG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAGTCACGAATGCAACTTCGCCATCAGTGCCCGTGGCATCGGCCGC
TTCCGCGTCAGTGCCTTCTACCAGCGCAACCTGGCCGGCATGGTGCTGCGCCGCATCGAGACCAACATCCCGACTCTGGA
TGACCTCAAGCTGCCGGAAATCCTCAAGAAGCTGGCGTTGACCAAGCGTGGCCTGGTGCTGTTCGTCGGCGCCACCGGTA
CCGGTAAATCGACCTCGCTGGCGGCGATGATCGGTTACCGCAACAAGAACAGCAGCGGCCACATCATCTCCATCGAAGAC
CCGATCGAGTACATCCACCAGCACCAGAACTGCATCGTCACCCAGCGCGAGGTCGGTATCGACACCGAATCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATTCTGATTGGTGAGGTGCGCACCCGCGAGACCATGGATCACG
CCGTGGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGCCGACCGGCAGAATCAGGTGTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GTTGATTCCAACCCCGGATGGCAAGGGGCGCCGCGCGGTGATCGAGGTGTTGATCAATACCCCGCTGGCGGCCGATCTGA
TCCGCAAGGGCGATGTGCACGAACTCAAGAACTTGATGAAGCGCTCCACCGAACTGGGTATGCAGACCTTCGATCAGGCG
CTTTACAGCCTCTATAGCCAGGGCGAAATCACCTACGAAGATGCGCTGCTCTACGCCGACTCGGCGAACGATTTGCGCCT
GCTGATCAAGCTCGGTTCGGAAGCCGACGGCGACCACCTGGCCCACCTGAGCGATGGTCTGTCACTGGAAGTCGCCCCCG
ATGACCCCGGCCGGCGTTTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

91.076

100

0.911
  pilU Acinetobacter baylyi ADP1

64.986

93.701

0.609
  pilU Vibrio cholerae strain A1552

58

91.864

0.533
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.254

93.176

0.394
  pilT Neisseria gonorrhoeae MS11

40

90.551

0.362
  pilT Neisseria meningitidis 8013

40

90.551

0.362

Multiple sequence alignment