Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Mag101_RS01080 Genome accession   NZ_CP019650
Coordinates   260582..261616 (-) Length   344 a.a.
NCBI ID   WP_077399583.1    Uniprot ID   A0A1Q2M163
Organism   Microbulbifer agarilyticus strain GP101     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 255582..266616
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Mag101_RS01060 (Mag101_01090) - 256022..257710 (-) 1689 WP_077399574.1 amidohydrolase -
  Mag101_RS01065 (Mag101_01095) - 257743..258360 (-) 618 WP_077399577.1 LysE family translocator -
  Mag101_RS01070 (Mag101_01100) - 258376..259122 (-) 747 WP_077407963.1 SDR family oxidoreductase -
  Mag101_RS01075 (Mag101_01105) pilU 259218..260366 (-) 1149 WP_077399580.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Mag101_RS01080 (Mag101_01110) pilT 260582..261616 (-) 1035 WP_077399583.1 type IV pilus twitching motility protein PilT Machinery gene
  Mag101_RS01085 (Mag101_01115) - 261715..262410 (+) 696 WP_077399586.1 YggS family pyridoxal phosphate-dependent enzyme -
  Mag101_RS01090 (Mag101_01120) - 262496..264532 (-) 2037 WP_077399589.1 alpha/beta hydrolase family protein -
  Mag101_RS01095 (Mag101_01125) proC 264796..265632 (+) 837 WP_232325085.1 pyrroline-5-carboxylate reductase -
  Mag101_RS01100 (Mag101_01130) - 265645..266250 (+) 606 WP_077399596.1 YggT family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38438.09 Da        Isoelectric Point: 6.4349

>NTDB_id=217558 Mag101_RS01080 WP_077399583.1 260582..261616(-) (pilT) [Microbulbifer agarilyticus strain GP101]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDVRRINLPPMEHKQVHGLIYEIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMEDLGMGQVFKQISDTPRGLVLVTGPTGSGKSTSLAAMIDYINDNKYEHILTVED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKAMVRSMLSESLQAVISQTLLKRNGGGRVAAHEIMRGTPAIRNLIREDKIAQMYSAIQTGANVGMQTMDQCL
QDLLEKRIITREVAREKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=217558 Mag101_RS01080 WP_077399583.1 260582..261616(-) (pilT) [Microbulbifer agarilyticus strain GP101]
ATGGACATTACAGAACTCCTCGCCTTCAGCGCCAAGCAGAACGCCTCGGATTTGCACCTCTCCGCCGGCCTGCCCCCCAT
GATCCGGGTAGATGGCGATGTTAGGCGCATCAACCTGCCGCCTATGGAGCACAAACAGGTCCACGGTCTGATCTACGAGA
TCATGAACGACAAGCAGCGCAAGGACTTCGAAGAGTTCCTGGAAACCGACTTCTCCTTCGAAGTCCCCGGTGTGGCCCGC
TTCCGTGTGAACGCGTTCAACCACAACCGCGGTGCCGGCGCCGTATTCCGGACCATTCCCTCCAAGGTACTCACCATGGA
AGACCTGGGGATGGGCCAAGTGTTCAAGCAGATCTCCGACACCCCGCGCGGCCTGGTACTGGTGACCGGCCCTACCGGTT
CCGGTAAGTCCACCTCGCTGGCGGCGATGATCGACTACATCAACGACAACAAATACGAGCACATCCTCACCGTAGAGGAC
CCGATCGAATTCGTACACGAATCCAAGAAGTGCCTGGTCAACCAGCGGGAAGTGCACCGCGATACCCACGGCTTCTCCGA
AGCACTGCGCTCCGCACTGCGTGAAGACCCGGACATCATTCTGGTTGGTGAGATGCGAGACCTGGAAACCATCCGCCTGG
CACTGACCGCCGCGGAAACCGGTCACCTGGTATTCGGCACCCTGCACACCACCTCCGCCGCCAAGACCATCGACCGTGTC
GTCGATGTATTCCCCGCGGAAGAAAAGGCCATGGTGCGCTCCATGCTCTCGGAATCGTTGCAGGCAGTAATCTCCCAGAC
CCTGCTCAAGCGCAACGGCGGCGGCCGAGTGGCGGCCCACGAAATCATGCGCGGCACCCCGGCGATCCGGAACCTGATCC
GCGAAGACAAGATCGCGCAGATGTACTCCGCCATCCAGACCGGTGCCAACGTAGGCATGCAGACCATGGACCAGTGCCTG
CAGGACCTGCTTGAGAAACGCATCATCACTCGCGAAGTCGCGCGTGAAAAAGCGAAGATGCCGGAGAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Q2M163

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

88.953

100

0.89
  pilT Pseudomonas stutzeri DSM 10701

88.081

100

0.881
  pilT Acinetobacter nosocomialis M2

83.43

100

0.834
  pilT Acinetobacter baumannii D1279779

83.43

100

0.834
  pilT Acinetobacter baumannii strain A118

83.43

100

0.834
  pilT Acinetobacter baylyi ADP1

80.523

100

0.805
  pilT Legionella pneumophila strain Lp02

75.148

98.256

0.738
  pilT Legionella pneumophila strain ERS1305867

75.148

98.256

0.738
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.353

98.837

0.666
  pilT Vibrio cholerae strain A1552

67.353

98.837

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392
  pilU Vibrio cholerae strain A1552

40.923

94.477

0.387

Multiple sequence alignment