Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BZG29_RS05245 Genome accession   NZ_CP019510
Coordinates   1184308..1185351 (-) Length   347 a.a.
NCBI ID   WP_070258779.1    Uniprot ID   A0A1G8GTH2
Organism   Janthinobacterium sp. LM6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1179308..1190351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BZG29_RS05230 (BZG29_05240) - 1180381..1182492 (-) 2112 WP_077399666.1 EAL domain-containing protein -
  BZG29_RS05235 (BZG29_05245) - 1182678..1183163 (-) 486 WP_077399669.1 glutathione peroxidase -
  BZG29_RS05240 (BZG29_05250) pilU 1183165..1184304 (-) 1140 WP_171984942.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BZG29_RS05245 (BZG29_05255) pilT 1184308..1185351 (-) 1044 WP_070258779.1 type IV pilus twitching motility protein PilT Machinery gene
  BZG29_RS05250 (BZG29_05260) - 1185422..1186123 (+) 702 WP_077399672.1 YggS family pyridoxal phosphate-dependent enzyme -
  BZG29_RS05255 (BZG29_05265) proC 1186146..1186976 (+) 831 WP_077399676.1 pyrroline-5-carboxylate reductase -
  BZG29_RS05260 (BZG29_05270) - 1186989..1187705 (-) 717 WP_077399680.1 anti-sigma factor -
  BZG29_RS05265 (BZG29_05275) - 1187702..1188259 (-) 558 WP_253192787.1 sigma-70 family RNA polymerase sigma factor -
  BZG29_RS05270 (BZG29_05280) - 1188490..1189047 (-) 558 WP_077399685.1 DUF3455 domain-containing protein -
  BZG29_RS05275 (BZG29_05285) - 1189137..1189484 (-) 348 WP_077399688.1 hypothetical protein -
  BZG29_RS05280 (BZG29_05290) - 1189481..1189867 (-) 387 WP_046683403.1 phage holin family protein -
  BZG29_RS05285 (BZG29_05295) - 1189918..1190274 (-) 357 WP_077399691.1 DUF883 family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38059.57 Da        Isoelectric Point: 6.6380

>NTDB_id=216091 BZG29_RS05245 WP_070258779.1 1184308..1185351(-) (pilT) [Janthinobacterium sp. LM6]
MDISELLAFSVSNKASDLHLSSGLPPMIRVNGDVRRLNVAPLEHKEVHSMIYDIMNDSQRKAYEEALECDFSFEIPGLAR
FRVNAYNQERGASAVLRTIPSKVLTLEELNAPRIFGELAMRPRGLVLVTGPTGSGKSTTLAAMVNHVNERLNHHILTIED
PIEFVHEPKKCLINQREVGSHTHSFSNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKSIDRI
IDVFPAEEKEMVRAMLSESLQAVISQNLLKTKDGAGRVAAHEIMLATPAVRNLIREAKVAQMYSAIQTGSNVGMQTLDQC
LTDLVRRGTISAETARSAAKAPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=216091 BZG29_RS05245 WP_070258779.1 1184308..1185351(-) (pilT) [Janthinobacterium sp. LM6]
ATGGACATCTCCGAACTTCTCGCTTTCTCCGTCAGCAACAAGGCTTCCGACCTGCACCTGTCTTCCGGCCTGCCGCCGAT
GATACGGGTCAATGGCGACGTGCGCCGCCTCAACGTGGCCCCGCTCGAGCACAAGGAAGTGCACAGCATGATCTACGACA
TCATGAACGACAGCCAGCGCAAGGCGTATGAAGAAGCGCTGGAATGCGATTTCTCGTTCGAGATTCCCGGCCTGGCGCGC
TTCCGCGTGAACGCCTACAACCAGGAACGCGGCGCCTCGGCCGTGCTGCGCACGATTCCCTCGAAGGTGCTGACCCTGGA
AGAGCTGAACGCGCCGCGCATCTTCGGCGAACTGGCCATGCGCCCGCGGGGACTCGTGCTGGTGACGGGCCCGACGGGTT
CCGGCAAGTCGACCACCCTGGCGGCGATGGTCAACCACGTCAATGAACGGCTGAACCACCATATATTGACCATCGAAGAC
CCGATCGAATTCGTGCATGAACCGAAAAAATGCCTGATCAACCAGCGCGAAGTGGGCTCGCACACGCACTCGTTCAGCAA
CGCGCTGCGCTCGGCCCTGCGCGAAGACCCGGATGTGATTCTGGTGGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CCTTGACGGCGGCCGAGACGGGCCACCTGGTGTTCGGCACCCTGCACACCTCGTCGGCGGCGAAATCGATCGACCGCATC
ATCGACGTCTTCCCCGCCGAGGAAAAGGAAATGGTGCGCGCCATGCTGTCCGAATCCTTGCAAGCCGTCATTTCGCAAAA
CTTGCTCAAAACCAAGGACGGCGCGGGCCGCGTGGCCGCCCATGAAATCATGCTGGCCACGCCGGCCGTACGCAACCTGA
TCCGCGAAGCGAAAGTGGCGCAAATGTATTCGGCCATCCAGACGGGCAGCAACGTGGGCATGCAGACCCTGGACCAGTGC
CTCACCGACCTGGTGCGGCGCGGCACGATCTCGGCGGAAACGGCCCGCTCGGCCGCCAAGGCGCCTGAAAACTTCCCAGG
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1G8GTH2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Acinetobacter nosocomialis M2

75.362

99.424

0.749
  pilT Acinetobacter baumannii D1279779

75.362

99.424

0.749
  pilT Acinetobacter baumannii strain A118

75.362

99.424

0.749
  pilT Pseudomonas stutzeri DSM 10701

74.203

99.424

0.738
  pilT Acinetobacter baylyi ADP1

73.913

99.424

0.735
  pilT Legionella pneumophila strain Lp02

71.681

97.695

0.7
  pilT Legionella pneumophila strain ERS1305867

71.681

97.695

0.7
  pilT Neisseria meningitidis 8013

68.986

99.424

0.686
  pilT Neisseria gonorrhoeae MS11

68.696

99.424

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.326

97.118

0.683
  pilT Vibrio cholerae strain A1552

70.326

97.118

0.683
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

99.424

0.522
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406
  pilU Vibrio cholerae strain A1552

40.58

99.424

0.403

Multiple sequence alignment