Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PspR76_RS28800 Genome accession   NZ_CP019428
Coordinates   6345026..6346060 (-) Length   344 a.a.
NCBI ID   WP_159960649.1    Uniprot ID   -
Organism   Pseudomonas sp. R76     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6340026..6351060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PspR76_RS28765 (PspR76_29280) ruvX 6340120..6340557 (+) 438 WP_003195067.1 Holliday junction resolvase RuvX -
  PspR76_RS28770 (PspR76_29285) pyrR 6340630..6341136 (+) 507 WP_159960637.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  PspR76_RS28775 (PspR76_29290) - 6341161..6342165 (+) 1005 WP_159960639.1 aspartate carbamoyltransferase catalytic subunit -
  PspR76_RS28780 (PspR76_29295) - 6342162..6343433 (+) 1272 WP_159960641.1 dihydroorotase -
  PspR76_RS28790 (PspR76_29300) - 6343551..6343979 (-) 429 WP_159960645.1 TM2 domain-containing protein -
  PspR76_RS28795 (PspR76_29305) - 6344229..6344870 (+) 642 WP_159960647.1 C40 family peptidase -
  PspR76_RS28800 (PspR76_29310) pilT 6345026..6346060 (-) 1035 WP_159960649.1 type IV pilus twitching motility protein PilT Machinery gene
  PspR76_RS28805 (PspR76_29315) - 6346118..6346804 (+) 687 WP_159960651.1 YggS family pyridoxal phosphate-dependent enzyme -
  PspR76_RS28810 (PspR76_29320) proC 6346838..6347656 (+) 819 WP_159960653.1 pyrroline-5-carboxylate reductase -
  PspR76_RS28815 (PspR76_29325) - 6347696..6348283 (+) 588 WP_159960655.1 YggT family protein -
  PspR76_RS28820 (PspR76_29330) - 6348480..6349619 (+) 1140 WP_159960657.1 homoserine O-acetyltransferase -
  PspR76_RS28825 (PspR76_29335) metW 6349627..6350247 (+) 621 WP_016972957.1 methionine biosynthesis protein MetW -
  PspR76_RS28830 (PspR76_29340) - 6350269..6350703 (+) 435 WP_159960660.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37353.24 Da        Isoelectric Point: 6.9840

>NTDB_id=215701 PspR76_RS28800 WP_159960649.1 6345026..6346060(-) (pilT) [Pseudomonas sp. R76]
MDITELLTASVRRGASDLHLSAGLAPMLRVDGEVWPMDGPALLPPQVADLLSPLLNRCQQKDFETSLETDFAFELPSVAR
FRVNVFQQDRGMGAVFRTIPEQVQSLESLGLGEVFQRIAQLPRGLVLVTGPTGSGKSTTLAAMIDYLNSHRRQHILTLED
PIEFIHRPKMALINQRQVHRDTHSFAAALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTVDRL
VDVFPAGEKAMVRSMLSESLQAVVSQVLVKKIGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KALVGEGVISREVARGQARVPGDI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=215701 PspR76_RS28800 WP_159960649.1 6345026..6346060(-) (pilT) [Pseudomonas sp. R76]
ATGGATATCACTGAATTGCTGACGGCCAGCGTGCGCCGTGGCGCCTCCGACCTGCATTTGTCCGCCGGCCTGGCGCCGAT
GCTGCGTGTCGATGGCGAGGTCTGGCCAATGGATGGCCCGGCGCTTTTGCCCCCGCAAGTTGCGGATTTATTGAGCCCTT
TGCTCAACCGATGCCAGCAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCCTTCGAGCTGCCGAGTGTGGCGCGG
TTCCGCGTGAATGTGTTCCAGCAGGATCGCGGCATGGGCGCGGTGTTTCGTACCATTCCTGAGCAAGTGCAGAGCCTGGA
AAGCCTCGGGCTCGGCGAGGTGTTCCAGCGTATCGCCCAGCTGCCGCGCGGCCTGGTGTTGGTGACCGGCCCGACCGGGT
CCGGCAAGTCCACGACGCTGGCGGCCATGATCGACTACCTCAACAGTCACCGGCGCCAGCACATCCTCACCCTGGAAGAC
CCCATCGAATTTATCCACAGGCCGAAAATGGCCTTGATCAACCAGCGTCAGGTGCACCGCGATACCCACAGCTTTGCCGC
GGCGCTGCGTTCGGCCCTGCGCGAAGACCCGGATGTAATCCTGGTGGGCGAGTTGCGCGACCTGGAGACCATCCGCCTGG
CCCTGACAGCCGCTGAAACCGGGCATCTGGTATTTGGCACCTTGCACACTACGTCGGCGGCCAAGACCGTGGACCGGCTG
GTGGACGTGTTCCCGGCAGGGGAAAAAGCCATGGTGCGCTCGATGCTTTCGGAGTCATTGCAGGCGGTGGTGTCGCAAGT
GCTGGTGAAGAAGATCGGCGGCGGGCGGGTGGCGGCTCACGAAATCATGCTGGGCACGCCGGCGATTCGTAACCTGATCC
GCGAAGACAAGGTGGCGCAAATGGTCTCGGCGATTCAGACCGGCGGGGCGTTGGGGATGAAGACGTTGGATATGAGTTTG
AAGGCGTTGGTCGGCGAGGGTGTGATCAGCCGGGAGGTGGCGCGGGGGCAGGCGAGGGTGCCTGGGGACATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

73.9

99.128

0.733
  pilT Pseudomonas stutzeri DSM 10701

71.848

99.128

0.712
  pilT Acinetobacter baumannii D1279779

71.261

99.128

0.706
  pilT Acinetobacter baumannii strain A118

71.261

99.128

0.706
  pilT Acinetobacter nosocomialis M2

71.261

99.128

0.706
  pilT Acinetobacter baylyi ADP1

70.674

99.128

0.701
  pilT Legionella pneumophila strain Lp02

64.793

98.256

0.637
  pilT Legionella pneumophila strain ERS1305867

64.793

98.256

0.637
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.261

95.64

0.634
  pilT Vibrio cholerae strain A1552

66.261

95.64

0.634
  pilT Neisseria meningitidis 8013

62.941

98.837

0.622
  pilT Neisseria gonorrhoeae MS11

62.353

98.837

0.616
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.298

97.674

0.491
  pilU Vibrio cholerae strain A1552

40

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

39.13

100

0.392
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387
  pilB Acinetobacter baumannii D1279779

31.552

100

0.36

Multiple sequence alignment