Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BST96_RS06100 Genome accession   NZ_CP019343
Coordinates   1356619..1357653 (-) Length   344 a.a.
NCBI ID   WP_085757839.1    Uniprot ID   -
Organism   Oceanicoccus sagamiensis strain NBRC 107125     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1351619..1362653
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BST96_RS06075 (BST96_06085) gshB 1352021..1352977 (+) 957 WP_085757834.1 glutathione synthase -
  BST96_RS06080 (BST96_06090) - 1353073..1353981 (+) 909 WP_085757835.1 energy transducer TonB -
  BST96_RS06085 (BST96_06095) - 1354297..1354851 (+) 555 WP_420814628.1 YqgE/AlgH family protein -
  BST96_RS06090 (BST96_06100) ruvX 1354855..1355283 (+) 429 WP_085757837.1 Holliday junction resolvase RuvX -
  BST96_RS06095 (BST96_06105) pilU 1355421..1356572 (-) 1152 WP_085757838.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BST96_RS06100 (BST96_06110) pilT 1356619..1357653 (-) 1035 WP_085757839.1 type IV pilus twitching motility protein PilT Machinery gene
  BST96_RS06105 (BST96_06115) - 1357842..1358528 (+) 687 WP_085757840.1 YggS family pyridoxal phosphate-dependent enzyme -
  BST96_RS06110 (BST96_06120) proC 1358594..1359424 (+) 831 WP_085757841.1 pyrroline-5-carboxylate reductase -
  BST96_RS06115 (BST96_06125) - 1359474..1360061 (+) 588 WP_085757842.1 YggT family protein -
  BST96_RS06120 (BST96_06130) metX 1360097..1361269 (+) 1173 WP_085757843.1 homoserine O-succinyltransferase MetX -
  BST96_RS06125 (BST96_06135) metW 1361269..1361859 (+) 591 WP_085757844.1 methionine biosynthesis protein MetW -
  BST96_RS06130 (BST96_06140) - 1361872..1362309 (+) 438 WP_085757845.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38285.95 Da        Isoelectric Point: 6.1865

>NTDB_id=214954 BST96_RS06100 WP_085757839.1 1356619..1357653(-) (pilT) [Oceanicoccus sagamiensis strain NBRC 107125]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPMEHKQVHSLIYEIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEELGMGQVFRDVSMVPRGLVLVTGPTGSGKSTTLAAMMDYINDNKYEHVLTIED
PIEFVHESKKCLVNQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAEEKSMVRSMLSESLQAVVAQTLLKKAGGGRVAAHEIMIGTSAIRNLIREDKVAQMYSAIQTGGSVGMQTMDQCL
TDLIDKRLISRDVAREKARFPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=214954 BST96_RS06100 WP_085757839.1 1356619..1357653(-) (pilT) [Oceanicoccus sagamiensis strain NBRC 107125]
ATGGATATTACTGAACTGTTAGCCTTTAGCGCCAAGCAAGGTGCTTCGGATTTACACCTTTCTGCGGGTTTGCCTCCTAT
GATCCGGGTGGATGGTGATGTCCGGCGGATTAATTTGCCGCCGATGGAGCACAAGCAGGTCCATAGCCTGATCTATGAGA
TTATGAACGATAAGCAGCGCAAGGACTTTGAAGAGTTTCTGGAAACGGATTTCTCCTTTGAAGTCCCCGGTGTGGCGCGC
TTCCGGGTTAACGCCTTTAACCAGAACCGCGGTGCCGGTGCGGTATTTCGGACCATCCCCTCTAAAGTACTATCGATGGA
AGAGTTGGGTATGGGGCAGGTATTTCGCGATGTTTCTATGGTGCCCAGAGGCTTGGTGCTGGTAACGGGGCCTACCGGTT
CTGGTAAGTCCACGACGCTGGCAGCGATGATGGACTATATCAATGACAATAAATACGAACATGTACTGACCATTGAAGAC
CCGATCGAATTTGTCCATGAATCGAAAAAGTGTCTGGTCAACCAGCGTGAGGTGCACCGTGATACCCACGGTTTTAATGA
AGCTCTGCGTTCAGCACTGCGTGAAGATCCCGATATTATTCTGGTGGGCGAGCTGCGAGATTTGGAAACCATCCGCCTGG
CGATGACCGCGGCAGAAACCGGTCACCTGGTTTTTGGTACGCTGCACACCACCTCGGCGGCAAAAACCATTGACCGTATT
ATTGATGTATTCCCCGCGGAAGAAAAATCGATGGTGCGTTCGATGCTGTCGGAATCCTTGCAGGCGGTCGTTGCGCAAAC
ACTATTGAAAAAAGCAGGCGGCGGCCGTGTGGCTGCCCATGAAATTATGATTGGTACGTCTGCTATTCGTAATCTGATCC
GTGAAGATAAAGTGGCACAAATGTATTCCGCGATTCAAACCGGTGGTTCTGTCGGTATGCAAACGATGGATCAATGTCTG
ACGGATTTAATTGATAAGCGTTTAATTAGTCGCGACGTAGCTCGCGAGAAAGCTCGATTCCCGGAAAACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

88.663

100

0.887
  pilT Pseudomonas stutzeri DSM 10701

88.081

100

0.881
  pilT Acinetobacter nosocomialis M2

81.395

100

0.814
  pilT Acinetobacter baumannii D1279779

81.395

100

0.814
  pilT Acinetobacter baumannii strain A118

81.395

100

0.814
  pilT Acinetobacter baylyi ADP1

79.651

100

0.797
  pilT Legionella pneumophila strain Lp02

76.923

98.256

0.756
  pilT Legionella pneumophila strain ERS1305867

76.923

98.256

0.756
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.301

95.64

0.663
  pilT Vibrio cholerae strain A1552

69.301

95.64

0.663
  pilT Neisseria meningitidis 8013

65.797

100

0.66
  pilT Neisseria gonorrhoeae MS11

65.217

100

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Vibrio cholerae strain A1552

40.719

97.093

0.395
  pilU Pseudomonas stutzeri DSM 10701

41.975

94.186

0.395
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392

Multiple sequence alignment