Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BV404_RS12285 Genome accession   NZ_CP019118
Coordinates   2633174..2634211 (-) Length   345 a.a.
NCBI ID   WP_024374563.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 12605     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2628174..2639211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BV404_RS12255 - 2628404..2629105 (+) 702 WP_075988971.1 endonuclease -
  BV404_RS12260 rsmE 2629202..2629933 (+) 732 WP_032080467.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  BV404_RS12265 gshB 2629946..2630902 (+) 957 WP_024374566.1 glutathione synthase -
  BV404_RS12270 - 2630942..2631505 (+) 564 WP_032080468.1 YqgE/AlgH family protein -
  BV404_RS12275 ruvX 2631567..2631992 (+) 426 WP_075988972.1 Holliday junction resolvase RuvX -
  BV404_RS12280 pilU 2632056..2633162 (-) 1107 WP_075988973.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BV404_RS12285 pilT 2633174..2634211 (-) 1038 WP_024374563.1 type IV pilus twitching motility protein PilT Machinery gene
  BV404_RS12290 - 2634237..2634944 (+) 708 WP_044366738.1 YggS family pyridoxal phosphate-dependent enzyme -
  BV404_RS12295 proC 2634981..2635799 (+) 819 WP_075988974.1 pyrroline-5-carboxylate reductase -
  BV404_RS12300 - 2635851..2636408 (+) 558 WP_075988975.1 YggT family protein -
  BV404_RS12305 yggU 2636408..2636698 (+) 291 WP_024374559.1 DUF167 family protein YggU -
  BV404_RS12310 - 2636758..2637189 (+) 432 WP_032080472.1 DUF4426 domain-containing protein -
  BV404_RS12315 - 2637213..2637812 (+) 600 WP_032080473.1 XTP/dITP diphosphatase -
  BV404_RS12320 hemW 2637812..2638984 (+) 1173 WP_075988977.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38227.83 Da        Isoelectric Point: 6.5038

>NTDB_id=213284 BV404_RS12285 WP_024374563.1 2633174..2634211(-) (pilT) [Vibrio fluvialis strain 12605]
MDISELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHADVHRLIFEIMNDAQRSEYEEKLEVDFSFALPDIGR
FRVNAFHQSRGASAVFRTIPTVIPTLDQLEAPEIFHKIANYEKGLVLVTGPTGSGKSTTLAAMVDYINATHNKHVLTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRLGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGASHGMQTMEQHA
KQLIAQGLVDPAEVQKKIELEMSAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=213284 BV404_RS12285 WP_024374563.1 2633174..2634211(-) (pilT) [Vibrio fluvialis strain 12605]
ATGGATATCTCTGAGTTACTGGAATTTAGTGTAAAGCATAATGCGTCAGATCTACATCTTTCTGCGGGTGTTCCTCCTAT
GGTACGCATCGATGGTGAAGTGAGAAAGCTTGGCGTACCTGCGTTTACACACGCTGATGTGCATCGATTAATTTTTGAAA
TTATGAATGATGCCCAGCGCAGTGAGTACGAAGAAAAGCTGGAAGTGGACTTTTCCTTTGCGTTACCGGACATTGGCCGA
TTTCGTGTCAACGCTTTCCACCAGTCGCGCGGTGCGTCTGCGGTATTTCGTACCATTCCGACCGTGATCCCGACTCTGGA
TCAGCTTGAGGCTCCGGAAATTTTCCACAAAATCGCCAATTACGAAAAAGGTTTGGTGCTGGTGACCGGACCGACCGGCT
CGGGTAAATCAACCACGCTGGCGGCGATGGTCGATTACATCAACGCAACCCACAACAAGCATGTGCTGACCATCGAAGAT
CCGATTGAGTTTGTGCACAGCAACAACAAATGCTTGATCAACCAGCGTGAAGTACACCGCGACACCCACAGTTTTAAAAA
CGCGCTGCGCAGCGCATTGCGTGAAGATCCGGATGTGATTCTGGTCGGTGAGCTGCGCGATCAGGAAACCATCAGCTTAG
CGCTGACGGCGGCGGAAACCGGCCACTTGGTGTTCGGCACGCTGCACACCAGTTCGGCGGCGAAAACCATTGACCGCATC
ATCGATGTGTTCCCGGGCAGCGACAAGGACATGGTACGTTCAATGTTGTCAGAATCGCTGCGTGCGGTGATTGCGCAGAA
GCTGCTCAAACGTCTGGGTGGCGGGCGTGTGGCGTGTCATGAAATTATGTTGGCGACGCCTGCTATCCGTAACCTGATTC
GCGAAGACAAAGTCGCGCAGATGTATTCGATCATTCAGACTGGCGCCTCCCATGGAATGCAGACTATGGAGCAGCATGCC
AAACAGTTGATCGCGCAGGGGCTGGTGGATCCGGCGGAAGTGCAGAAGAAAATTGAACTGGAAATGTCCGCGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

92.754

100

0.928
  pilT Vibrio cholerae strain A1552

92.754

100

0.928
  pilT Acinetobacter baumannii D1279779

71.601

95.942

0.687
  pilT Acinetobacter baumannii strain A118

71.601

95.942

0.687
  pilT Acinetobacter nosocomialis M2

71.299

95.942

0.684
  pilT Acinetobacter baylyi ADP1

71.515

95.652

0.684
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.681

0.672
  pilT Pseudomonas aeruginosa PAK

67.059

98.551

0.661
  pilT Legionella pneumophila strain Lp02

67.173

95.362

0.641
  pilT Legionella pneumophila strain ERS1305867

67.173

95.362

0.641
  pilT Neisseria meningitidis 8013

62.059

98.551

0.612
  pilT Neisseria gonorrhoeae MS11

61.765

98.551

0.609
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.424

95.652

0.501
  pilU Vibrio cholerae strain A1552

42.769

94.203

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.867

96.232

0.403
  pilU Acinetobacter baylyi ADP1

40.312

92.754

0.374

Multiple sequence alignment