Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BV404_RS12280 Genome accession   NZ_CP019118
Coordinates   2632056..2633162 (-) Length   368 a.a.
NCBI ID   WP_075988973.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 12605     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2627056..2638162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BV404_RS12250 - 2627785..2628285 (+) 501 WP_075988970.1 SprT family zinc-dependent metalloprotease -
  BV404_RS12255 - 2628404..2629105 (+) 702 WP_075988971.1 endonuclease -
  BV404_RS12260 rsmE 2629202..2629933 (+) 732 WP_032080467.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  BV404_RS12265 gshB 2629946..2630902 (+) 957 WP_024374566.1 glutathione synthase -
  BV404_RS12270 - 2630942..2631505 (+) 564 WP_032080468.1 YqgE/AlgH family protein -
  BV404_RS12275 ruvX 2631567..2631992 (+) 426 WP_075988972.1 Holliday junction resolvase RuvX -
  BV404_RS12280 pilU 2632056..2633162 (-) 1107 WP_075988973.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BV404_RS12285 pilT 2633174..2634211 (-) 1038 WP_024374563.1 type IV pilus twitching motility protein PilT Machinery gene
  BV404_RS12290 - 2634237..2634944 (+) 708 WP_044366738.1 YggS family pyridoxal phosphate-dependent enzyme -
  BV404_RS12295 proC 2634981..2635799 (+) 819 WP_075988974.1 pyrroline-5-carboxylate reductase -
  BV404_RS12300 - 2635851..2636408 (+) 558 WP_075988975.1 YggT family protein -
  BV404_RS12305 yggU 2636408..2636698 (+) 291 WP_024374559.1 DUF167 family protein YggU -
  BV404_RS12310 - 2636758..2637189 (+) 432 WP_032080472.1 DUF4426 domain-containing protein -
  BV404_RS12315 - 2637213..2637812 (+) 600 WP_032080473.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41215.14 Da        Isoelectric Point: 6.0934

>NTDB_id=213283 BV404_RS12280 WP_075988973.1 2632056..2633162(-) (pilU) [Vibrio fluvialis strain 12605]
MQLEECLQGMMTYKASDLYITVGAPVLYRVDGELRAQGEKLDEAQVSALLAAMMDEARHADYLTTREANFAIVRDVGRFR
VSAFFQRELPGAVIRRIETQIPTFDELKLPAVLKDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNTHRSGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEVLLNSPRVSDIIRRGDLHELKSTMAKSKEVGMQTFDQALY
QLVVENKISEQDALHCADSANDLRLMLKTQRGDVSSSGSLDHVQIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=213283 BV404_RS12280 WP_075988973.1 2632056..2633162(-) (pilU) [Vibrio fluvialis strain 12605]
ATGCAACTCGAGGAGTGCCTGCAAGGCATGATGACGTATAAAGCGTCAGATCTATATATTACTGTGGGCGCACCCGTGCT
GTACCGGGTCGATGGTGAATTGCGCGCGCAGGGTGAGAAGCTGGATGAAGCGCAGGTCAGCGCGCTGCTGGCTGCGATGA
TGGATGAGGCGCGCCATGCGGACTATCTGACGACGCGTGAAGCTAACTTTGCCATCGTACGTGATGTGGGGCGCTTTCGC
GTCAGCGCTTTTTTTCAGCGCGAGCTACCCGGTGCGGTGATTCGCCGCATCGAAACGCAGATTCCGACCTTTGATGAACT
CAAACTGCCAGCAGTGCTGAAAGATCTCTCGATTGCCAAACGCGGTTTGGTGCTGGTGGTGGGGGCGACAGGGTCGGGCA
AATCGACCACCATGGCCGCGATGACTGGCTATCGCAACACGCATCGCAGCGGACATATTCTCACCGTCGAAGATCCGATT
GAATTTGTCCATGAGCACCAGCGTTGTATCGTGACACAGCGTGAAGTGGGATTGGACACCGAAAGCTATGAAGTGGCGCT
GAAGAACTCGCTACGCCAGGCGCCGGATATGATTTTGATCGGTGAGATCCGCAGCCGTGAAACCATGGAATACGCAATGA
CCTTCGCCGAAACCGGTCACTTATGCATGGCAACTCTGCACGCCAACAACGCCAACCAAGCGCTCGAACGTATTTTGCAT
CTGGTGCCGAAAGAGCAGCGTGAACAATTTCTGTTTGACCTGTCGATGAACCTCAAAGGTGTTGTCGGTCAGCAACTGAT
CCGCGATAAAAACGGGCAGGGACGTCACGGCGTGTTTGAAGTGTTGCTCAACAGTCCACGGGTGTCGGACATCATCCGAC
GCGGCGATTTGCATGAGCTGAAAAGCACCATGGCGAAATCGAAAGAGGTCGGCATGCAAACCTTTGATCAGGCGTTGTAT
CAGTTGGTGGTCGAGAACAAGATCAGCGAACAGGATGCGCTGCACTGTGCCGATTCGGCCAACGATCTGCGCTTGATGCT
CAAGACTCAGCGGGGCGATGTGAGCAGCAGCGGTTCACTGGATCATGTGCAAATCGACATGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

85.326

100

0.853
  pilU Pseudomonas stutzeri DSM 10701

57.143

95.109

0.543
  pilU Acinetobacter baylyi ADP1

53.846

95.38

0.514
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44

88.315

0.389
  pilT Acinetobacter baylyi ADP1

41.349

92.663

0.383
  pilT Legionella pneumophila strain Lp02

41.298

92.12

0.38
  pilT Legionella pneumophila strain ERS1305867

41.298

92.12

0.38
  pilT Pseudomonas aeruginosa PAK

41.493

91.033

0.378
  pilT Acinetobacter baumannii strain A118

41.003

92.12

0.378
  pilT Acinetobacter baumannii D1279779

41.003

92.12

0.378
  pilT Acinetobacter nosocomialis M2

41.003

92.12

0.378
  pilT Pseudomonas stutzeri DSM 10701

40

91.033

0.364

Multiple sequence alignment