Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Ga0123462_RS06605 Genome accession   NZ_CP018800
Coordinates   1359694..1360848 (+) Length   384 a.a.
NCBI ID   WP_100265578.1    Uniprot ID   A0A2K8L7G9
Organism   Mariprofundus ferrinatatus strain CP-8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1354694..1365848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Ga0123462_RS06580 (Ga0123462_1331) - 1354818..1355549 (-) 732 WP_100265574.1 TSUP family transporter -
  Ga0123462_RS06585 (Ga0123462_1332) - 1355687..1356895 (-) 1209 WP_100265575.1 UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase -
  Ga0123462_RS06590 (Ga0123462_1333) - 1356895..1358124 (-) 1230 WP_100265576.1 FAD-dependent oxidoreductase -
  Ga0123462_RS06600 (Ga0123462_1335) pilT 1358599..1359651 (+) 1053 WP_100265577.1 type IV pilus twitching motility protein PilT Machinery gene
  Ga0123462_RS06605 (Ga0123462_1336) pilU 1359694..1360848 (+) 1155 WP_100265578.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Ga0123462_RS06610 (Ga0123462_1337) - 1360848..1361939 (+) 1092 WP_100265579.1 type IV pilus twitching motility protein PilT -
  Ga0123462_RS06615 (Ga0123462_1338) - 1361936..1362571 (-) 636 WP_100265580.1 lysophospholipid acyltransferase family protein -
  Ga0123462_RS06620 (Ga0123462_1339) - 1362719..1363621 (+) 903 WP_100265581.1 hypothetical protein -
  Ga0123462_RS06625 (Ga0123462_1340) - 1363655..1364620 (-) 966 WP_100265582.1 DUF6765 family protein -

Sequence

Protein


Download         Length: 384 a.a.        Molecular weight: 42781.08 Da        Isoelectric Point: 6.1876

>NTDB_id=210308 Ga0123462_RS06605 WP_100265578.1 1359694..1360848(+) (pilU) [Mariprofundus ferrinatatus strain CP-8]
MSAAETSLSIKQLLQVMEKREASDLYITTGMPPSYRINGVVQPLKQPPLSALQCEKLATSIMNERQRASFAAEYEMNMAL
AYPDIGRFRVNIFRQRGHIGMVIRKVKTEVPTIDQLGLPEIFKDIAMSPRGLVLMVGTTGSGKSTSLAAMVDWRNSNQAG
HIISIEDPIEFVHEHKKSVITQREVGTDTLEYHKALKNALRQAPDVILIGEIRDRETMEHALEFSETGHLCMATLHANNA
NQALERIINFFPEEMHPQVCLNLALNLKSILSQRLVKTPADRRVAAIEILINTPRVSDLIGKWDIAEIKETMASGGNYGM
QTFDQHLLSLWANGVISEDEALRQADSVNNLRLQIKMVKLEDQGGSANDLEQLSNDGSSEGLRI

Nucleotide


Download         Length: 1155 bp        

>NTDB_id=210308 Ga0123462_RS06605 WP_100265578.1 1359694..1360848(+) (pilU) [Mariprofundus ferrinatatus strain CP-8]
ATGAGTGCTGCTGAAACATCCCTGTCGATCAAGCAGCTGCTGCAGGTGATGGAGAAACGCGAGGCATCCGACCTCTATAT
CACGACCGGCATGCCCCCCTCCTACCGCATTAATGGCGTTGTTCAGCCGTTGAAACAACCACCACTGAGCGCACTGCAGT
GCGAAAAGCTGGCCACCTCAATCATGAACGAACGCCAGCGCGCCTCCTTTGCCGCCGAATATGAGATGAATATGGCGCTC
GCCTACCCCGACATCGGACGATTCCGCGTCAATATCTTCCGCCAGCGCGGCCATATCGGCATGGTTATTCGCAAAGTGAA
AACGGAAGTTCCGACCATTGATCAACTGGGACTGCCCGAGATTTTCAAGGATATTGCCATGTCTCCGCGTGGGCTGGTAC
TGATGGTCGGCACCACCGGTTCAGGAAAATCCACCTCACTGGCGGCCATGGTTGACTGGCGCAATTCGAATCAGGCGGGC
CATATTATTTCCATCGAAGACCCGATTGAATTTGTGCATGAACACAAGAAGAGCGTCATCACCCAGCGCGAGGTCGGCAC
CGACACACTGGAGTATCACAAAGCGCTGAAGAACGCCCTCAGACAGGCACCCGATGTAATCCTGATCGGAGAGATTCGTG
ACCGTGAAACCATGGAGCATGCGCTCGAATTCTCTGAAACCGGCCACCTCTGCATGGCAACCCTGCATGCCAACAACGCA
AATCAGGCGCTGGAGCGCATAATCAACTTCTTCCCTGAGGAGATGCATCCGCAGGTGTGTCTGAATCTGGCCCTGAATCT
CAAATCCATTCTATCCCAGAGGCTGGTAAAAACGCCTGCCGACAGACGTGTTGCCGCCATTGAGATCCTGATCAATACGC
CGCGCGTTTCCGATCTGATCGGAAAATGGGATATTGCCGAGATTAAAGAGACAATGGCGAGCGGCGGCAACTACGGTATG
CAGACCTTCGACCAGCACCTGCTCAGTCTCTGGGCGAATGGCGTCATTAGCGAGGATGAGGCACTGCGCCAGGCCGACTC
GGTTAACAACCTGCGGCTGCAGATCAAGATGGTGAAACTTGAGGATCAGGGCGGCAGTGCAAATGACCTTGAGCAGTTGT
CGAATGATGGCAGCTCGGAAGGGCTGAGAATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(49-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K8L7G9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.646

98.438

0.518
  pilU Acinetobacter baylyi ADP1

53.409

91.667

0.49
  pilU Vibrio cholerae strain A1552

50.416

94.01

0.474
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.868

88.802

0.398
  pilT Pseudomonas aeruginosa PAK

43.895

89.583

0.393
  pilT Pseudomonas stutzeri DSM 10701

43.314

89.583

0.388
  pilT Acinetobacter baumannii D1279779

44.311

86.979

0.385
  pilT Acinetobacter baumannii strain A118

44.311

86.979

0.385
  pilT Acinetobacter nosocomialis M2

44.012

86.979

0.383
  pilT Neisseria gonorrhoeae MS11

42.773

88.281

0.378
  pilT Neisseria meningitidis 8013

42.773

88.281

0.378
  pilT Acinetobacter baylyi ADP1

43.333

85.938

0.372
  pilT Legionella pneumophila strain ERS1305867

42.814

86.979

0.372
  pilT Legionella pneumophila strain Lp02

42.814

86.979

0.372
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.643

86.719

0.37
  pilT Vibrio cholerae strain A1552

42.643

86.719

0.37

Multiple sequence alignment