Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Ga0123462_RS06600 Genome accession   NZ_CP018800
Coordinates   1358599..1359651 (+) Length   350 a.a.
NCBI ID   WP_100265577.1    Uniprot ID   A0A2K8L4E6
Organism   Mariprofundus ferrinatatus strain CP-8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1353599..1364651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Ga0123462_RS06580 (Ga0123462_1331) - 1354818..1355549 (-) 732 WP_100265574.1 TSUP family transporter -
  Ga0123462_RS06585 (Ga0123462_1332) - 1355687..1356895 (-) 1209 WP_100265575.1 UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase -
  Ga0123462_RS06590 (Ga0123462_1333) - 1356895..1358124 (-) 1230 WP_100265576.1 FAD-dependent oxidoreductase -
  Ga0123462_RS06600 (Ga0123462_1335) pilT 1358599..1359651 (+) 1053 WP_100265577.1 type IV pilus twitching motility protein PilT Machinery gene
  Ga0123462_RS06605 (Ga0123462_1336) pilU 1359694..1360848 (+) 1155 WP_100265578.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Ga0123462_RS06610 (Ga0123462_1337) - 1360848..1361939 (+) 1092 WP_100265579.1 type IV pilus twitching motility protein PilT -
  Ga0123462_RS06615 (Ga0123462_1338) - 1361936..1362571 (-) 636 WP_100265580.1 lysophospholipid acyltransferase family protein -
  Ga0123462_RS06620 (Ga0123462_1339) - 1362719..1363621 (+) 903 WP_100265581.1 hypothetical protein -
  Ga0123462_RS06625 (Ga0123462_1340) - 1363655..1364620 (-) 966 WP_100265582.1 DUF6765 family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38904.01 Da        Isoelectric Point: 7.5456

>NTDB_id=210307 Ga0123462_RS06600 WP_100265577.1 1358599..1359651(+) (pilT) [Mariprofundus ferrinatatus strain CP-8]
MEVSELLAFTVKNKASDLHLSSGEPPMIRINGDMTRIKMPPVDKRDIQSMVYDIMSDTQRKVFEEHMELDFSFALGEIAR
FRVNVFQQRRGMGAVFRVIPSEVLTLENLKCPDIFKDIAMTPRGICLVTGPTGSGKSTTLAAMIDHRNERERGHILTIED
PIEFVHKSKNCLISQRELGPHTHSFENALRSALREDPDVILVGELRDLETIRLALTAAETGHMVFGTLHTSSAPKTIDRV
IDVFPAAEKEMVRSMLSESIRAVISQTLLKTADGNSRIAAHEIMIGTPAIRNLIRENKIAQMVSVMQTSLRDGMQTLDSN
LQSLVNARKITQQAAMEKAANKKLFGGGAI

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=210307 Ga0123462_RS06600 WP_100265577.1 1358599..1359651(+) (pilT) [Mariprofundus ferrinatatus strain CP-8]
ATGGAAGTCTCAGAGCTGCTTGCCTTTACCGTAAAGAACAAAGCCTCGGATCTGCACCTCTCATCTGGCGAACCGCCGAT
GATCCGCATCAACGGCGACATGACTCGCATCAAGATGCCTCCGGTCGACAAACGCGATATCCAGTCGATGGTCTACGACA
TCATGAGCGACACCCAGCGCAAGGTGTTCGAGGAGCATATGGAGCTGGATTTCTCATTTGCACTCGGTGAGATCGCCCGT
TTCCGTGTCAACGTATTCCAGCAGCGGCGCGGCATGGGTGCGGTATTCCGCGTCATTCCAAGCGAAGTACTGACCCTTGA
AAACCTCAAGTGCCCTGATATTTTCAAGGATATCGCCATGACCCCGCGCGGTATCTGTCTGGTGACAGGCCCGACCGGTT
CGGGCAAATCCACAACGCTTGCCGCCATGATCGATCACCGCAATGAGCGTGAACGCGGACATATACTGACCATTGAAGAC
CCGATTGAGTTTGTACATAAATCGAAAAACTGCCTGATCTCCCAGCGTGAACTGGGACCACATACCCACTCGTTTGAAAA
CGCCCTGCGCAGTGCGCTTCGTGAAGACCCGGATGTCATTCTGGTCGGCGAATTGCGCGATCTGGAAACCATTCGGCTGG
CCCTGACCGCTGCCGAAACCGGGCACATGGTATTTGGCACACTGCACACCTCTTCAGCGCCCAAAACCATCGACCGTGTG
ATCGATGTATTTCCTGCTGCTGAAAAGGAGATGGTGCGATCCATGCTCTCCGAATCGATTCGGGCCGTCATCTCACAGAC
ACTGCTTAAAACAGCCGATGGCAACAGCCGTATTGCAGCCCATGAAATCATGATCGGCACACCCGCCATCCGAAACCTGA
TTCGCGAAAACAAGATCGCCCAGATGGTGTCGGTGATGCAGACATCACTTCGCGATGGCATGCAAACACTGGACTCAAAC
CTGCAGTCGCTGGTCAATGCAAGGAAAATAACCCAACAGGCTGCTATGGAAAAAGCGGCTAACAAGAAACTGTTCGGTGG
CGGTGCCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K8L4E6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

66.667

98.571

0.657
  pilT Acinetobacter baumannii D1279779

66.667

98.571

0.657
  pilT Acinetobacter baumannii strain A118

66.667

98.571

0.657
  pilT Pseudomonas stutzeri DSM 10701

65.797

98.571

0.649
  pilT Neisseria gonorrhoeae MS11

65.598

98

0.643
  pilT Neisseria meningitidis 8013

65.598

98

0.643
  pilT Pseudomonas aeruginosa PAK

65.782

96.857

0.637
  pilT Legionella pneumophila strain Lp02

64.348

98.571

0.634
  pilT Legionella pneumophila strain ERS1305867

64.348

98.571

0.634
  pilT Acinetobacter baylyi ADP1

66.769

92.857

0.62
  pilT Vibrio cholerae strain A1552

62.769

92.857

0.583
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

62.769

92.857

0.583
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

98.571

0.503
  pilU Acinetobacter baylyi ADP1

43.235

97.143

0.42
  pilU Pseudomonas stutzeri DSM 10701

41.888

96.857

0.406
  pilU Vibrio cholerae strain A1552

39.528

96.857

0.383

Multiple sequence alignment