Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IMCC3135_RS05800 Genome accession   NZ_CP018632
Coordinates   1355655..1356692 (+) Length   345 a.a.
NCBI ID   WP_088916743.1    Uniprot ID   A0A2Z2NL79
Organism   Granulosicoccus antarcticus IMCC3135     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1350655..1361692
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMCC3135_RS05775 (IMCC3135_05840) - 1350997..1351551 (-) 555 WP_088916739.1 HU family DNA-binding protein -
  IMCC3135_RS05780 (IMCC3135_05845) - 1351883..1352485 (-) 603 WP_088916740.1 YggT family protein -
  IMCC3135_RS05785 (IMCC3135_05850) proC 1352648..1353502 (-) 855 WP_088916741.1 pyrroline-5-carboxylate reductase -
  IMCC3135_RS05790 (IMCC3135_05855) tal 1353502..1354629 (-) 1128 WP_088916742.1 transaldolase -
  IMCC3135_RS05795 (IMCC3135_05860) - 1354743..1355447 (-) 705 WP_088921700.1 YggS family pyridoxal phosphate-dependent enzyme -
  IMCC3135_RS05800 (IMCC3135_05865) pilT 1355655..1356692 (+) 1038 WP_088916743.1 type IV pilus twitching motility protein PilT Machinery gene
  IMCC3135_RS05805 (IMCC3135_05870) pilU 1356747..1357928 (+) 1182 WP_088916744.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IMCC3135_RS05810 (IMCC3135_05875) - 1358060..1359304 (+) 1245 WP_088916745.1 galactokinase -
  IMCC3135_RS05815 (IMCC3135_05880) - 1359294..1360025 (+) 732 WP_088916746.1 sugar phosphate nucleotidyltransferase -
  IMCC3135_RS05820 (IMCC3135_05885) - 1360052..1361005 (-) 954 WP_088916747.1 exostosin domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38004.59 Da        Isoelectric Point: 6.4189

>NTDB_id=209178 IMCC3135_RS05800 WP_088916743.1 1355655..1356692(+) (pilT) [Granulosicoccus antarcticus IMCC3135]
MDIAQLLTFSVKNGASDMHLSAGLPPMIRVDGDMRRVNVPALDHAAVQAMVYDIMNDKQRKDFEEKMETDFSFEIPDTAR
FRVNAFNQNRGCGAVFRTIPSEVLTLEQLGAPKIFQEISSLPRGIVLVTGPTGSGKSTTLAAMMDYKNDSELGHILTIED
PIEFVHQSKKCLINQREVHRDTFGFSEALRSALREDPDVILVGEMRDMETISLALTAAETGHLVFGTLHTSSAAKTVDRI
VDVFPAGEKPMVRSMLSESLKAVISQTLLKKTGGGRIAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGQSHGMQTLDQNL
KELVKRGQVDLAEARKKAQNPDSLQ

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=209178 IMCC3135_RS05800 WP_088916743.1 1355655..1356692(+) (pilT) [Granulosicoccus antarcticus IMCC3135]
ATGGATATCGCTCAACTTCTGACATTCAGTGTCAAGAACGGTGCCTCGGATATGCATCTGTCAGCCGGCTTGCCGCCAAT
GATACGCGTGGATGGTGACATGCGGCGAGTCAACGTCCCTGCGCTAGACCACGCAGCTGTGCAGGCCATGGTGTATGACA
TCATGAATGACAAGCAGCGAAAAGACTTTGAAGAGAAGATGGAGACGGATTTCTCGTTTGAAATCCCCGATACCGCGCGT
TTTCGTGTCAACGCTTTCAATCAGAACCGAGGCTGCGGTGCGGTATTCCGGACGATCCCCTCCGAGGTGCTGACACTGGA
ACAACTGGGTGCTCCGAAGATCTTCCAGGAAATATCATCTTTGCCACGAGGTATTGTGCTGGTTACCGGTCCGACAGGCT
CAGGCAAGTCGACCACTCTGGCGGCGATGATGGATTACAAGAATGATTCAGAATTGGGGCATATCCTGACCATTGAGGAT
CCGATTGAATTTGTTCATCAAAGCAAAAAGTGCCTGATTAATCAGCGCGAAGTACATCGAGATACCTTTGGCTTTTCCGA
AGCTCTGCGCTCTGCACTTCGTGAAGATCCTGACGTTATTCTGGTTGGAGAAATGCGTGATATGGAAACCATCTCTCTGG
CATTGACGGCGGCTGAAACCGGTCACCTGGTATTCGGTACCCTGCACACCAGTTCTGCAGCGAAGACCGTGGATCGAATC
GTGGATGTGTTCCCGGCTGGTGAAAAACCGATGGTGCGCTCGATGTTGTCCGAATCGTTGAAGGCTGTCATATCGCAGAC
ACTGTTGAAAAAGACCGGCGGCGGACGAATCGCTGCACATGAAATCATGATTGGTACGCCGGCGATTCGTAACCTGATTC
GTGAAGACAAGGTGGCCCAGATGTATTCGGCTATCCAGACCGGCCAGTCACATGGTATGCAAACACTGGATCAGAACCTT
AAAGAGCTGGTAAAGCGCGGGCAGGTGGATCTTGCCGAGGCTCGTAAAAAAGCTCAAAACCCCGACAGCTTGCAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z2NL79

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.907

99.71

0.777
  pilT Acinetobacter baumannii D1279779

77.907

99.71

0.777
  pilT Acinetobacter baumannii strain A118

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

77.616

99.71

0.774
  pilT Pseudomonas stutzeri DSM 10701

77.326

99.71

0.771
  pilT Acinetobacter baylyi ADP1

76.163

99.71

0.759
  pilT Legionella pneumophila strain Lp02

71.176

98.551

0.701
  pilT Legionella pneumophila strain ERS1305867

71.176

98.551

0.701
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.796

98.261

0.696
  pilT Vibrio cholerae strain A1552

70.796

98.261

0.696
  pilT Neisseria meningitidis 8013

68.406

100

0.684
  pilT Neisseria gonorrhoeae MS11

68.116

100

0.681
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.877

99.13

0.504
  pilU Vibrio cholerae strain A1552

40.751

100

0.409
  pilU Acinetobacter baylyi ADP1

40.058

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.173

100

0.403

Multiple sequence alignment