Detailed information NTG0209179

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IMCC3135_RS05805
• Genome accession: NZ_CP018632
• Coordinates: 1356747..1357928 (+)
• Length: 393 a.a.
• NCBI ID: WP_088916744.1
• Uniprot ID: -
• Organism: Granulosicoccus antarcticus IMCC3135
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1351747..1362928

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IMCC3135_RS05780 (IMCC3135_05845)	-	1351883..1352485 (-)	603	WP_088916740.1	YggT family protein	-
IMCC3135_RS05785 (IMCC3135_05850)	proC	1352648..1353502 (-)	855	WP_088916741.1	pyrroline-5-carboxylate reductase	-
IMCC3135_RS05790 (IMCC3135_05855)	tal	1353502..1354629 (-)	1128	WP_088916742.1	transaldolase	-
IMCC3135_RS05795 (IMCC3135_05860)	-	1354743..1355447 (-)	705	WP_088921700.1	YggS family pyridoxal phosphate-dependent enzyme	-
IMCC3135_RS05800 (IMCC3135_05865)	pilT	1355655..1356692 (+)	1038	WP_088916743.1	type IV pilus twitching motility protein PilT	Machinery gene
IMCC3135_RS05805 (IMCC3135_05870)	pilU	1356747..1357928 (+)	1182	WP_088916744.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IMCC3135_RS05810 (IMCC3135_05875)	-	1358060..1359304 (+)	1245	WP_088916745.1	galactokinase	-
IMCC3135_RS05815 (IMCC3135_05880)	-	1359294..1360025 (+)	732	WP_088916746.1	sugar phosphate nucleotidyltransferase	-
IMCC3135_RS05820 (IMCC3135_05885)	-	1360052..1361005 (-)	954	WP_088916747.1	exostosin domain-containing protein	-

Sequence

Protein

• Length: 393 a.a.
• Molecular weight: 43063.78 Da
• Isoelectric Point: 7.3367

>NTDB_id=209179 IMCC3135_RS05805 WP_088916744.1 1356747..1357928(+) (pilU) [Granulosicoccus antarcticus IMCC3135]
MDLKVAKRFMDELLVSLHKKGGSDLFIIAGAPPSMRIHGKVTPVMEKKLTAEHTLALTQCLMTDAERADFKKTKECNFAI
ALPKISRFRVNALVQRGSAGLVVRIIPNEIPSFADLHMPDILTQVAMSKRGLVIFVGGTGSGKSTSLAAMIDYRNENSNG
HIITIEDPVEFVHHHKGCIVTQREVGTDTESFEIALKNTLRQAPDVILIGEIRDQKTMEHAIAFAETGHLCLATLHANNT
NQALDRIINFFPETRHKQLLLDLSLNVKAFISQRLLPTPSGKGRRAAVEVLLNTPLMSDMIMKGEVHEIKGLMKKSKDQG
MQTFDQALFDLLGKNAITIEEAMRNADSVNDLRLRIKLAGGGSSEVDDMISNSMSLEDDGMDDASGGGARKTG

Nucleotide

• Length: 1182 bp

>NTDB_id=209179 IMCC3135_RS05805 WP_088916744.1 1356747..1357928(+) (pilU) [Granulosicoccus antarcticus IMCC3135]
ATGGATTTGAAGGTCGCCAAGCGATTCATGGATGAGCTGTTAGTCAGTCTGCACAAGAAAGGCGGGTCTGATCTGTTCAT
CATCGCCGGAGCTCCGCCTTCGATGCGTATCCACGGCAAGGTAACTCCTGTCATGGAGAAGAAGCTGACCGCTGAACATA
CTCTGGCGCTGACGCAATGCCTCATGACCGACGCCGAGCGTGCGGATTTCAAGAAAACCAAGGAGTGCAATTTCGCCATT
GCTCTGCCCAAGATTTCTCGATTTCGTGTCAACGCTCTGGTGCAGCGGGGCAGTGCGGGTCTGGTTGTGCGAATCATTCC
TAACGAGATACCCAGCTTCGCCGACCTGCATATGCCCGATATTCTCACTCAGGTAGCCATGAGCAAGCGTGGTCTGGTCA
TTTTTGTGGGTGGTACCGGTTCGGGCAAGTCCACCAGTCTTGCGGCAATGATCGACTACCGGAATGAAAATTCCAACGGT
CACATCATCACCATTGAGGATCCGGTGGAATTTGTGCATCACCACAAGGGTTGTATTGTCACCCAGCGTGAGGTCGGCAC
GGATACCGAGTCGTTTGAAATTGCTCTGAAGAACACATTGCGACAGGCACCCGATGTCATTCTCATCGGAGAAATACGCG
ATCAGAAGACGATGGAGCACGCCATTGCATTTGCCGAGACCGGGCATCTGTGTCTGGCCACCTTGCACGCGAACAATACC
AATCAGGCGCTGGACAGAATCATCAACTTTTTCCCAGAAACCAGGCACAAGCAGTTATTGCTTGACCTGTCTTTGAATGT
CAAGGCTTTCATTTCACAGCGCTTGTTGCCTACGCCCAGTGGTAAAGGGCGTCGAGCCGCGGTGGAAGTGCTACTCAATA
CGCCGTTGATGTCGGACATGATCATGAAAGGTGAAGTTCACGAGATCAAGGGCCTGATGAAGAAGAGTAAGGATCAGGGA
ATGCAGACCTTTGATCAGGCGCTGTTCGACCTGCTCGGTAAAAACGCCATCACTATCGAAGAGGCCATGCGTAATGCGGA
TTCGGTGAACGATCTGCGTCTGCGTATCAAGCTTGCTGGCGGCGGTTCATCAGAGGTGGATGACATGATCAGCAATTCGA
TGAGCCTGGAGGATGACGGTATGGATGATGCCTCCGGTGGCGGTGCGCGAAAAACCGGATAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (46-275)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	60.105	96.947	0.583
pilU	Acinetobacter baylyi ADP1	58.333	91.603	0.534
pilU	Vibrio cholerae strain A1552	52.989	93.639	0.496