Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   CLD04_RS14250 Genome accession   NZ_CP023257
Coordinates   2654890..2655894 (-) Length   334 a.a.
NCBI ID   WP_003229718.1    Uniprot ID   O32055
Organism   Bacillus subtilis strain TLO3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2649890..2660894
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLD04_RS14215 (CLD04_14215) yrbG 2650708..2651364 (-) 657 WP_017696351.1 DUF421 domain-containing protein -
  CLD04_RS14220 (CLD04_14220) - 2651562..2651951 (+) 390 WP_029727131.1 TIGR04086 family membrane protein -
  CLD04_RS14230 (CLD04_14230) yajC 2652163..2652429 (-) 267 WP_004398708.1 preprotein translocase subunit YajC -
  CLD04_RS14235 (CLD04_14235) tgt 2652467..2653612 (-) 1146 WP_003229725.1 tRNA guanosine(34) transglycosylase Tgt -
  CLD04_RS14240 (CLD04_14240) queA 2653639..2654667 (-) 1029 WP_003229723.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  CLD04_RS14245 (CLD04_14245) yrzS 2654697..2654897 (-) 201 WP_095843862.1 DUF2905 domain-containing protein -
  CLD04_RS14250 (CLD04_14250) ruvB 2654890..2655894 (-) 1005 WP_003229718.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  CLD04_RS14255 (CLD04_14255) ruvA 2655905..2656510 (-) 606 WP_003229717.1 Holliday junction branch migration protein RuvA Machinery gene
  CLD04_RS14260 (CLD04_14260) bofC 2656649..2657161 (-) 513 WP_003229715.1 sporulation cell-cell signaling protein BofC -
  CLD04_RS14265 (CLD04_14265) csbX 2657209..2658516 (-) 1308 WP_003246197.1 MFS transporter -
  CLD04_RS14270 (CLD04_14270) iolG 2658587..2659612 (-) 1026 WP_041339258.1 inositol 2-dehydrogenase -
  CLD04_RS14275 (CLD04_14275) yrzF 2659850..2660487 (+) 638 Protein_2784 serine/threonine protein kinase -
  CLD04_RS21690 - 2660533..2660655 (-) 123 WP_003229707.1 hypothetical protein -
  CLD04_RS22165 - 2660740..2660814 (+) 75 Protein_2786 hypothetical protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37467.78 Da        Isoelectric Point: 5.1631

>NTDB_id=205803 CLD04_RS14250 WP_003229718.1 2654890..2655894(-) (ruvB) [Bacillus subtilis strain TLO3]
MDERLVSSEADNHESVIEQSLRPQNLAQYIGQHKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE
LRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT
RVGLLTAPLRDRFGVMSRLEYYTQEELADIVTRTADVFEVEIDKPSALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI
TEDISQNALERLQVDRLGLDHIDHKLLMGMIEKFNGGPVGLDTISATIGEESHTIEDVYEPYLLQIGFIQRTPRGRIVTP
AVYHHFQMEAPRYD

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=205803 CLD04_RS14250 WP_003229718.1 2654890..2655894(-) (ruvB) [Bacillus subtilis strain TLO3]
ATGGATGAACGGCTCGTCTCAAGTGAAGCAGACAACCATGAATCAGTGATAGAGCAAAGCCTGAGGCCGCAAAATCTGGC
ACAGTATATCGGGCAGCATAAGGTAAAAGAAAATTTGCGCGTGTTTATAGATGCGGCGAAAATGAGACAGGAGACGCTGG
ATCATGTTCTTCTGTACGGGCCGCCCGGACTCGGAAAAACAACGCTTGCTTCGATTGTTGCTAATGAAATGGGAGTGGAA
TTGCGGACGACATCAGGGCCTGCGATTGAAAGGCCTGGTGATTTGGCTGCTATATTAACGGCGCTGGAACCGGGAGATGT
ATTATTTATTGATGAAATACATAGGCTGCACAGATCGATCGAAGAGGTGCTTTATCCTGCAATGGAAGACTTTTGCCTGG
ACATTGTGATTGGCAAAGGCCCATCTGCCCGCTCGGTTCGGCTCGACCTTCCGCCTTTTACGCTGGTAGGAGCCACGACG
AGGGTGGGGCTTTTGACGGCTCCTTTAAGAGACAGATTCGGCGTCATGTCTCGCCTTGAGTACTATACACAAGAAGAACT
GGCGGATATTGTCACAAGAACCGCTGATGTATTCGAGGTTGAGATTGATAAACCGTCGGCGCTTGAAATTGCAAGAAGAT
CGAGAGGGACGCCCCGCGTGGCGAACCGCCTGCTGAGAAGGGTGCGCGATTTCGCCCAAGTGCTGGGTGACAGCAGGATC
ACTGAAGACATTTCTCAGAATGCGCTGGAGCGGCTTCAGGTTGATCGCCTCGGTTTGGATCATATTGATCATAAGCTGCT
GATGGGCATGATCGAAAAATTCAACGGCGGCCCAGTAGGCCTTGATACGATATCAGCTACGATCGGCGAGGAATCGCATA
CGATTGAAGACGTATATGAACCATATCTGCTGCAAATTGGATTTATACAAAGAACGCCGCGTGGAAGGATCGTGACACCG
GCCGTTTATCATCATTTCCAAATGGAGGCTCCCCGATATGACTGA

Domains

Predicted by InterProScan.

RuvB AAA lid domain (AAA_lid_4)

(182-255)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)

RuvB C-terminal winged helix domain (WHD_RuvB)

(257-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O32055

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

100

100

1
  ruvB Streptococcus pneumoniae TIGR4

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae R6

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae D39

60.429

97.605

0.59
  ruvB Synechocystis sp. PCC 6803

57.143

98.503

0.563
  ruvB Helicobacter pylori 26695

52.744

98.204

0.518

Multiple sequence alignment