Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BO996_RS05485 Genome accession   NZ_CP018101
Coordinates   1193095..1194138 (-) Length   347 a.a.
NCBI ID   WP_012202999.1    Uniprot ID   A0A210VX04
Organism   Delftia sp. HK171     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1188095..1199138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BO996_RS05455 (BO996_05395) - 1188110..1188526 (+) 417 WP_043820402.1 YraN family protein -
  BO996_RS05460 (BO996_05400) - 1188600..1189208 (+) 609 WP_012202994.1 SIS domain-containing protein -
  BO996_RS05465 (BO996_05405) - 1189205..1189936 (+) 732 WP_063327425.1 BON domain-containing protein -
  BO996_RS05470 (BO996_05410) - 1190136..1191047 (-) 912 WP_013804430.1 NAD(P)-dependent oxidoreductase -
  BO996_RS05475 (BO996_05415) pilU 1191173..1192309 (-) 1137 WP_012202997.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BO996_RS05480 (BO996_05420) - 1192431..1193063 (-) 633 WP_012202998.1 cyclic nucleotide-binding domain-containing protein -
  BO996_RS05485 (BO996_05425) pilT 1193095..1194138 (-) 1044 WP_012202999.1 type IV pilus twitching motility protein PilT Machinery gene
  BO996_RS05490 (BO996_05430) - 1194176..1194901 (+) 726 WP_071954210.1 YggS family pyridoxal phosphate-dependent enzyme -
  BO996_RS05495 (BO996_05435) - 1194940..1196223 (-) 1284 WP_071954211.1 branched-chain amino acid ABC transporter substrate-binding protein -
  BO996_RS05500 (BO996_05440) - 1196250..1196972 (-) 723 WP_013804427.1 TetR/AcrR family transcriptional regulator -
  BO996_RS05505 (BO996_05445) - 1197132..1198730 (+) 1599 WP_071954212.1 AMP-binding protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38309.02 Da        Isoelectric Point: 6.8352

>NTDB_id=205803 BO996_RS05485 WP_012202999.1 1193095..1194138(-) (pilT) [Delftia sp. HK171]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDPLDHKTVHAMVYDIMSDSQRKAYEEFLEVDFSFEIEGLAR
FRVNAFNQYRGAAAVFRTIPSKILTLEQLNAPRIFGDLALKPRGLVLVTGPTGSGKSTTLAGMVNHLNETEYGHILTVED
PIEFVHDSKKCLINQREVGPMTQSFSAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRIAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSQNVGMQTLDQN
LTDLVRRNLISPAEARAKAKIPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=205803 BO996_RS05485 WP_012202999.1 1193095..1194138(-) (pilT) [Delftia sp. HK171]
GTGGACATCACCCAATTGCTGGCATTCAGCGTCAAGAACAAGGCCTCCGACCTGCACCTGTCCGCGGGGCTGCCGCCGAT
GATCCGTGTGCACGGCGATGTGCGCCGCATCAATGTCGATCCGCTGGACCACAAGACGGTCCACGCCATGGTCTACGACA
TCATGAGCGACTCCCAGCGCAAGGCCTACGAGGAGTTCCTGGAGGTGGACTTCTCGTTCGAGATCGAGGGGCTGGCGCGC
TTCCGTGTCAACGCCTTCAACCAGTACCGGGGCGCGGCCGCCGTCTTCCGGACCATTCCCAGCAAGATCCTGACGCTGGA
GCAGCTCAACGCGCCGCGCATCTTCGGCGACCTGGCCCTGAAGCCGCGCGGCCTGGTGCTGGTGACCGGCCCCACGGGCT
CGGGCAAGTCCACCACGCTGGCCGGCATGGTCAACCACCTCAACGAGACCGAGTACGGGCACATCCTGACGGTGGAGGAC
CCCATCGAATTCGTGCACGACTCCAAGAAGTGCCTGATCAACCAGCGTGAAGTCGGGCCGATGACGCAGTCGTTCTCGGC
CGCCCTGCGCTCGGCGCTGCGCGAGGACCCGGACGCCATCCTGGTGGGCGAAATGCGTGACCTGGAAACCATCCGCCTGG
CCATGACCGCGGCCGAGACGGGCCACCTGGTCTTCGGCACCCTGCACACCTCCAGCGCCGCCAAGACCATCGACCGCATC
ATCGACGTCTTCCCCGCCGAGGAAAAGGAGATGGTGCGCGCCATGCTCTCCGAATCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCATCGCGGCCCACGAAATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCCCAGATGTACTCCACCATCCAGACGAGTCAGAATGTGGGCATGCAGACGCTGGACCAGAAC
CTGACCGATCTGGTGCGGCGCAACCTGATCAGCCCTGCGGAAGCCCGCGCCAAGGCCAAGATACCGGACAACTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A210VX04

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Acinetobacter nosocomialis M2

75.362

99.424

0.749
  pilT Acinetobacter baumannii D1279779

75.362

99.424

0.749
  pilT Acinetobacter baumannii strain A118

75.362

99.424

0.749
  pilT Pseudomonas stutzeri DSM 10701

74.493

99.424

0.741
  pilT Acinetobacter baylyi ADP1

73.043

99.424

0.726
  pilT Legionella pneumophila strain Lp02

73.156

97.695

0.715
  pilT Legionella pneumophila strain ERS1305867

73.156

97.695

0.715
  pilT Neisseria meningitidis 8013

68.235

97.983

0.669
  pilT Neisseria gonorrhoeae MS11

67.941

97.983

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.276

98.271

0.651
  pilT Vibrio cholerae strain A1552

66.276

98.271

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

42.985

96.542

0.415
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398

Multiple sequence alignment