Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BO996_RS05475 Genome accession   NZ_CP018101
Coordinates   1191173..1192309 (-) Length   378 a.a.
NCBI ID   WP_012202997.1    Uniprot ID   A0A210VX48
Organism   Delftia sp. HK171     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1186173..1197309
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BO996_RS05450 (BO996_05390) rsmI 1187169..1188110 (-) 942 WP_013804433.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  BO996_RS05455 (BO996_05395) - 1188110..1188526 (+) 417 WP_043820402.1 YraN family protein -
  BO996_RS05460 (BO996_05400) - 1188600..1189208 (+) 609 WP_012202994.1 SIS domain-containing protein -
  BO996_RS05465 (BO996_05405) - 1189205..1189936 (+) 732 WP_063327425.1 BON domain-containing protein -
  BO996_RS05470 (BO996_05410) - 1190136..1191047 (-) 912 WP_013804430.1 NAD(P)-dependent oxidoreductase -
  BO996_RS05475 (BO996_05415) pilU 1191173..1192309 (-) 1137 WP_012202997.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BO996_RS05480 (BO996_05420) - 1192431..1193063 (-) 633 WP_012202998.1 cyclic nucleotide-binding domain-containing protein -
  BO996_RS05485 (BO996_05425) pilT 1193095..1194138 (-) 1044 WP_012202999.1 type IV pilus twitching motility protein PilT Machinery gene
  BO996_RS05490 (BO996_05430) - 1194176..1194901 (+) 726 WP_071954210.1 YggS family pyridoxal phosphate-dependent enzyme -
  BO996_RS05495 (BO996_05435) - 1194940..1196223 (-) 1284 WP_071954211.1 branched-chain amino acid ABC transporter substrate-binding protein -
  BO996_RS05500 (BO996_05440) - 1196250..1196972 (-) 723 WP_013804427.1 TetR/AcrR family transcriptional regulator -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41978.48 Da        Isoelectric Point: 6.9076

>NTDB_id=205802 BO996_RS05475 WP_012202997.1 1191173..1192309(-) (pilU) [Delftia sp. HK171]
MERDQATKFINDLLKLMVSRNGSDLFITAEFPPAIKIDGRITKVSPQPLSPVHTLTLARSVMSDKQIADFERTKECNFAI
SPPGIGRFRVNAFIQQGRVGMVMRTIPLALPTIDSLGVPQVLKEVAMTKRGLCILVGATGSGKSTTLAAMVDWRNESSYG
HIVTIEDPIEFVHPHKNCVVTQREVGLDTDSWDAALKNSLRQAPDVILMGEIRDRETMEHAIAFSETGHLCLATLHANSA
NQALDRIVNFFPEDRRPQLLMDLSLNLRAMVSQRLLPKQDSKGRVAAIEVMLNSPLISDLIFKGEVTEIKEIMKKSRNLG
MQTFDQALFDLFESNLVTYEDALRNADSVNDLRLQIKLGSQRAKSLDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=205802 BO996_RS05475 WP_012202997.1 1191173..1192309(-) (pilU) [Delftia sp. HK171]
ATGGAAAGAGATCAGGCCACCAAGTTCATCAACGACCTGCTCAAGCTCATGGTCAGCCGCAACGGCAGCGACTTGTTCAT
CACGGCGGAATTCCCGCCGGCGATCAAGATCGACGGGCGCATCACCAAGGTGTCGCCGCAGCCGCTGTCGCCCGTGCACA
CGCTGACGCTGGCGCGCTCGGTCATGAGCGACAAGCAGATCGCCGATTTCGAGCGCACCAAGGAGTGCAACTTCGCCATC
TCGCCGCCGGGCATAGGGCGCTTTCGCGTCAACGCCTTCATCCAGCAGGGCCGCGTGGGCATGGTGATGCGGACGATTCC
GCTGGCCCTGCCCACCATCGACAGCCTGGGCGTGCCCCAGGTGCTCAAGGAGGTGGCCATGACCAAGCGCGGCCTGTGCA
TCCTGGTCGGCGCCACGGGCTCGGGCAAATCCACCACGCTGGCCGCCATGGTGGACTGGCGCAACGAGAGCTCCTACGGC
CACATCGTGACCATCGAGGACCCCATCGAATTCGTGCACCCGCACAAGAACTGCGTGGTCACGCAGCGCGAAGTGGGCCT
GGACACGGACAGCTGGGACGCGGCTCTCAAGAACTCCCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAGCACGCCATCGCCTTCTCGGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCGTCAACTTCTTCCCCGAAGACCGCCGCCCACAGCTGTTGATGGACCTGTCGCTGAACCT
GCGCGCCATGGTCTCCCAGCGCCTGCTGCCCAAGCAGGACAGCAAGGGCCGCGTGGCCGCCATCGAGGTGATGCTCAACT
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGAGGTCACCGAGATCAAGGAGATCATGAAAAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGTCCAACCTGGTCACCTACGAGGATGCACTGCGCAATGCCGA
CTCGGTCAACGACCTGCGGCTGCAGATCAAGCTGGGCAGCCAGCGCGCCAAGTCGCTGGACCTGGCGGCGGGCACCGAGC
ATTTCGCCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A210VX48

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.324

92.328

0.585
  pilU Acinetobacter baylyi ADP1

56.825

94.974

0.54
  pilU Vibrio cholerae strain A1552

53.161

92.063

0.489
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.697

89.153

0.407
  pilT Pseudomonas aeruginosa PAK

43.62

89.153

0.389
  pilT Pseudomonas stutzeri DSM 10701

42.433

89.153

0.378
  pilT Legionella pneumophila strain ERS1305867

42.643

88.095

0.376
  pilT Legionella pneumophila strain Lp02

42.643

88.095

0.376
  pilT Acinetobacter nosocomialis M2

42.042

88.095

0.37
  pilT Acinetobacter baumannii D1279779

42.042

88.095

0.37
  pilT Acinetobacter baumannii strain A118

42.042

88.095

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.887

84.392

0.37
  pilT Vibrio cholerae strain A1552

43.887

84.392

0.37

Multiple sequence alignment