Detailed information    

experimental Experimentally validated

Overview

Name   recJ   Type   Machinery gene
Locus tag   SPV_RS02850 Genome accession   NZ_CP027540
Coordinates   543288..545510 (+) Length   740 a.a.
NCBI ID   WP_000632595.1    Uniprot ID   A0A0H2ZRL7
Organism   Streptococcus pneumoniae strain D39V     
Function   homologous recombination   
Homologous recombination

Function

The ssDNA-specific exonuclease, RecJ, works in combination with RecFOR to extend the ssDNA region, enhancing the ability of RecFOR to load RecA.


Genomic Context

Location: 538288..550510
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPV_RS02820 (SPV_0527) - 538641..539831 (-) 1191 WP_000843716.1 ferric reductase-like transmembrane domain-containing protein -
  SPV_RS02830 (SPV_0528) - 540089..540748 (-) 660 WP_000443862.1 amino acid ABC transporter permease -
  SPV_RS02835 (SPV_0529) - 540758..541435 (-) 678 WP_000131158.1 amino acid ABC transporter permease -
  SPV_RS02840 (SPV_0530) - 541448..542242 (-) 795 WP_000726170.1 transporter substrate-binding domain-containing protein -
  SPV_RS02845 (SPV_0531) - 542253..543011 (-) 759 Protein_537 amino acid ABC transporter ATP-binding protein -
  SPV_RS02850 (SPV_0532) recJ 543288..545510 (+) 2223 WP_000632595.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  SPV_RS02855 (SPV_0533) - 545685..547346 (+) 1662 WP_000065637.1 ribonuclease J -
  SPV_RS02860 (SPV_0534) - 547415..548194 (+) 780 WP_000290025.1 alpha/beta hydrolase family protein -
  SPV_RS02865 (SPV_0535) murM 548306..549526 (+) 1221 WP_001819885.1 peptidoglycan bridge formation alanyltransferase MurM -

Sequence

Protein


Download         Length: 740 a.a.        Molecular weight: 82480.96 Da        Isoelectric Point: 4.3483

>NTDB_id=661 SPV_RS02850 WP_000632595.1 543288..545510(+) (recJ) [Streptococcus pneumoniae strain D39V]
MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEE
GENILVYGDYDADGMTSASIVKESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQVELLDLVAIGTIADMVSLTDENR
ILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEETVGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKN
EERKEIVQSIYEEAKTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAVDIFEALDP
HRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNLDEELDLEALSLETVKSFERLAPFGMDNQKP
IFYIKNFQVESARTMGAGNAHLKLKISKGEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVE
GVQLFNIRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKNDIDKAYYLTGYGTRDQ
FAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFEELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKD
QEMMALGTVQEIYDFLMEKE

Nucleotide


Download         Length: 2223 bp        

>NTDB_id=661 SPV_RS02850 WP_000632595.1 543288..545510(+) (recJ) [Streptococcus pneumoniae strain D39V]
TTGATAACACCTACTTATGAATGGCAGTTTGCCCTGCAGGTAGAAGATGCGGATTTTACAAAGATAGCCAAGAAGGCTGG
ACTGGGTCCTGAGGTGGCTCGGTTATTGTTTGAGAGAGGGATTCAGGACCAAGAAAGTCTGAAGAAGTTTTTAGAACCTT
CCTTGGAGGACTTACATGATGCTTATCTGCTCCATGATATGGACAAGGCAGTGGAGCGGATTCGTCAGGCTATTGAAGAA
GGGGAAAATATTCTCGTTTATGGAGACTATGATGCGGATGGCATGACTTCGGCTTCTATTGTGAAGGAAAGTTTGGAACA
ACTTGGTGCTGAGTGCCGAGTTTACCTGCCAAATCGTTTTACCGATGGCTATGGCCCTAATGCTAGTGTTTATAAATACT
TTATCGAGCAAGAAGGAATTTCCTTGATTGTGACGGTGGACAATGGGGTTGCTGGTCATGAGGCTATTGCATTGGCTCAG
TCTATGGGAGTAGATGTCATTGTGACAGACCATCATTCCATGCCTGAAACCCTGCCAGATGCTTATGCTATTGTCCATCC
TGAACATCCAGATGCGGATTATCCTTTTAAATATTTGGCTGGTTGTGGAGTTGCTTTCAAGTTGGCTTGTGCCCTGTTAG
AAGAAGTGCAAGTGGAATTGCTTGATTTGGTCGCTATTGGAACTATTGCAGATATGGTGAGTCTGACGGATGAAAATCGT
ATCTTAGTTCAATATGGTCTGGAAATGTTGGGTCATACCCAGCGCATTGGTCTGCAAGAAATGCTGGACATGGCTGGGAT
TGCTGCCAACGAAGTAACAGAAGAAACGGTTGGTTTCCAGATTGCTCCTCGTTTGAATGCCTTGGGTCGCTTGGATGATC
CCAATCCTGCCATTGATTTGTTGACTGGATTTGATGATGAGGAAGCGCATGAGATTGCCCTTATGATTCACCAGAAAAAC
GAAGAGCGCAAGGAAATCGTTCAGTCTATCTATGAAGAAGCCAAGACCATGGTGGATCCTGAGAAGAAGGTTCAGGTCTT
GGCCAAGGAAGGCTGGAATCCTGGGGTTCTAGGAATCGTGGCTGGTCGTTTATTGGAAGAATTGGGACAGACAGTCATTG
TTCTTAATATAGAAGACGGTCGTGCCAAGGGCAGTGCTCGTAGTGTGGAAGCGGTCGATATTTTTGAAGCTCTGGATCCC
CATCGAGACCTCTTCATCGCCTTTGGAGGTCATGCAGGTGCAGCGGGTATGACGCTGGAAGTTGAGCAACTCTCAGATTT
ATCTCAGGTTTTGGAAGATTATGTTCGTGAAAAAGGTGCAGATGCTGGTGGCAAGAATAAGTTAAACCTAGATGAAGAGT
TGGATTTGGAGGCACTTAGCTTGGAAACGGTCAAAAGTTTTGAACGTTTAGCTCCTTTTGGAATGGATAATCAGAAACCT
ATTTTTTATATCAAGAATTTTCAGGTCGAAAGTGCTCGTACTATGGGGGCAGGTAATGCCCATCTAAAGCTGAAAATTTC
CAAGGGTGAGGCGAGTTTTGAAGTGGTAGCCTTTGGTCAAGGCAGATGGGCGACAGAGTTTTCTCAAACCAAGAATCTAG
AGTTAGCGGTTAAATTGTCTGTCAACCAATGGAATGGCCAAACTGCTCTCCAGTTGATGATGGTGGATGCGCGAGTGGAA
GGTGTTCAACTTTTTAACATTCGTGGAAAAAATGCAGTCTTGCCAGAAGGTGTTCCAGTCTTGGATTTTCCTGGAGAACT
GCCAAATCTTGCGGCTAGTGAAGCTGTTGTCGTAAAAAACATTCCAGAGGATATTACTCAGCTGAAGACCATTTTTCAGG
AACAGCATTTCTCTGCTGTCTATTTCAAAAATGATATTGACAAGGCTTATTATCTGACAGGTTATGGGACTAGAGATCAG
TTTGCCAAATTGTACAAGACTATTTACCAGTTCCCAGAGTTTGATATTCGCTACAAGCTGAAAGATTTGGCTGCATATCT
TAATATTCAACAAATCTTGCTGGTCAAGATGATTCAAGTATTTGAAGAACTAGGCTTTGTGACGATAAAAGATGGTGTGA
TGACAGTCAATAAAGAGGCGCCAAAGCGGGAGATAGGAGAAAGTCAAATTTACCAAAATCTCAAACAAACCGTTAAAGAC
CAAGAAATGATGGCGCTGGGTACGGTGCAAGAAATTTATGATTTTTTGATGGAAAAAGAGTAG

Domains

Predicted by InterProScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(593-735)

DHH family (DHH)

(83-228)

DHHA1 domain (DHHA1)

(317-434)

RecJ OB domain (RecJ_OB)

(451-556)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZRL7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

36.917

100

0.385

References

[1] Bevika Sewgoolam et al. (2025) Genome-wide antibiotic-CRISPRi profiling identifies LiaR activation as a strategy to resensitize fluoroquinolone-resistant Streptococcus pneumoniae. Nature Communications 16(1):6491. [PMID: 40659638]