Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BM528_RS14330 Genome accession   NZ_CP018031
Coordinates   3221475..3222518 (-) Length   347 a.a.
NCBI ID   WP_071980409.1    Uniprot ID   -
Organism   Alteromonas sp. RW2A1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3216475..3227518
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BM528_RS14295 (BM528_14080) - 3216591..3216863 (+) 273 WP_071980403.1 hypothetical protein -
  BM528_RS14300 (BM528_14085) - 3217036..3217764 (+) 729 WP_071980404.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  BM528_RS14305 (BM528_14090) gshB 3217893..3218846 (+) 954 WP_071981333.1 glutathione synthase -
  BM528_RS14310 (BM528_14095) - 3218888..3219454 (+) 567 WP_071980405.1 YqgE/AlgH family protein -
  BM528_RS14315 (BM528_14100) ruvX 3219447..3219902 (+) 456 WP_071980406.1 Holliday junction resolvase RuvX -
  BM528_RS14320 (BM528_14105) - 3219929..3220249 (-) 321 WP_071980407.1 DUF2007 domain-containing protein -
  BM528_RS14325 (BM528_14110) pilU 3220304..3221416 (-) 1113 WP_071980408.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BM528_RS14330 (BM528_14115) pilT 3221475..3222518 (-) 1044 WP_071980409.1 type IV pilus twitching motility protein PilT Machinery gene
  BM528_RS14335 (BM528_14120) - 3222546..3223232 (+) 687 WP_071980410.1 YggS family pyridoxal phosphate-dependent enzyme -
  BM528_RS14340 (BM528_14125) proC 3223408..3224229 (+) 822 WP_071980411.1 pyrroline-5-carboxylate reductase -
  BM528_RS14345 (BM528_14130) - 3224251..3224790 (+) 540 WP_071980412.1 YggT family protein -
  BM528_RS14350 (BM528_14135) - 3224941..3225333 (+) 393 WP_083595403.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38381.07 Da        Isoelectric Point: 6.4432

>NTDB_id=205168 BM528_RS14330 WP_071980409.1 3221475..3222518(-) (pilT) [Alteromonas sp. RW2A1]
MDITELLAFSVKNNASDLHLSAGLPPLIRVDGEMRKLNVPALDHKQVHGLVYEIMNDMQRKEYEENLETDFSFEVKGLSR
FRVNAFVQNRGAAAVLRTIPSDVLTLDDLGAPDIFKEIINQPTGIVLVTGATGSGKSTTLAAMVDHINTHKREHILTIED
PIEFVHDNKLSLVNQREVHRDTHSFNNALRSALREDPDVILVGELRDLETIRLAISAAETGHLVFGTLHTNSAPKTIDRI
IDVFPAEEKSMVRSMLSESLRAVISQTLLKKIGGGRVAAHEIMVGIPAIRNLIREDKVPQMYSVIQTGQAHGMQTMDQCL
QRLVAMGVITKQDAAAKSVDKQAMNSF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=205168 BM528_RS14330 WP_071980409.1 3221475..3222518(-) (pilT) [Alteromonas sp. RW2A1]
GTGGATATTACCGAACTTTTAGCGTTTAGTGTTAAGAATAACGCCTCAGATTTACACTTGTCAGCCGGACTTCCGCCGCT
TATTCGCGTAGACGGCGAAATGCGCAAGCTCAATGTCCCAGCACTTGACCACAAGCAGGTTCACGGGCTCGTTTATGAAA
TCATGAACGATATGCAACGTAAAGAATACGAGGAAAATCTAGAAACCGATTTTTCTTTTGAAGTAAAAGGGCTGTCGCGC
TTTCGTGTCAACGCGTTTGTTCAAAACCGCGGTGCCGCCGCTGTACTTCGTACTATTCCTAGCGACGTTCTGACTCTTGA
CGATTTAGGCGCGCCGGATATTTTTAAGGAAATTATTAATCAGCCAACCGGCATTGTTTTAGTAACCGGTGCAACGGGCT
CCGGTAAAAGTACTACACTAGCGGCCATGGTAGACCACATTAATACTCACAAGCGTGAGCACATTCTTACTATCGAAGAC
CCCATCGAATTTGTTCACGACAACAAACTTAGTTTGGTCAACCAGCGGGAAGTACATCGAGATACGCATTCATTTAATAA
TGCTTTGCGATCGGCATTACGTGAAGACCCTGACGTTATCCTTGTAGGCGAGCTTCGAGATTTAGAAACCATTCGACTAG
CTATATCAGCCGCAGAAACCGGCCATTTGGTATTTGGTACTTTGCACACTAACTCCGCGCCAAAAACTATTGACCGTATT
ATCGACGTGTTTCCTGCAGAAGAAAAATCGATGGTGCGTTCAATGTTATCAGAATCTTTGCGGGCGGTTATATCTCAAAC
GCTGCTCAAGAAAATAGGCGGTGGGCGTGTGGCAGCTCATGAGATAATGGTAGGAATACCGGCAATCAGAAACCTTATTC
GTGAAGACAAAGTCCCGCAAATGTATTCAGTGATTCAAACTGGACAGGCACACGGTATGCAAACCATGGATCAATGCTTA
CAACGGTTAGTGGCTATGGGTGTTATCACTAAGCAAGACGCCGCGGCAAAATCGGTTGACAAACAGGCCATGAATAGCTT
TTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

77.286

97.695

0.755
  pilT Acinetobacter baumannii D1279779

77.286

97.695

0.755
  pilT Acinetobacter nosocomialis M2

76.991

97.695

0.752
  pilT Pseudomonas stutzeri DSM 10701

74.203

99.424

0.738
  pilT Acinetobacter baylyi ADP1

75.298

96.83

0.729
  pilT Pseudomonas aeruginosa PAK

74.26

97.406

0.723
  pilT Legionella pneumophila strain Lp02

72.141

98.271

0.709
  pilT Legionella pneumophila strain ERS1305867

72.141

98.271

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.107

97.118

0.7
  pilT Vibrio cholerae strain A1552

72.107

97.118

0.7
  pilT Neisseria meningitidis 8013

64.74

99.712

0.646
  pilT Neisseria gonorrhoeae MS11

64.451

99.712

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.895

98.847

0.513
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406
  pilU Vibrio cholerae strain A1552

41.791

96.542

0.403
  pilU Acinetobacter baylyi ADP1

40.774

96.83

0.395

Multiple sequence alignment