Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BM528_RS14325 Genome accession   NZ_CP018031
Coordinates   3220304..3221416 (-) Length   370 a.a.
NCBI ID   WP_071980408.1    Uniprot ID   -
Organism   Alteromonas sp. RW2A1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3215304..3226416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BM528_RS14295 (BM528_14080) - 3216591..3216863 (+) 273 WP_071980403.1 hypothetical protein -
  BM528_RS14300 (BM528_14085) - 3217036..3217764 (+) 729 WP_071980404.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  BM528_RS14305 (BM528_14090) gshB 3217893..3218846 (+) 954 WP_071981333.1 glutathione synthase -
  BM528_RS14310 (BM528_14095) - 3218888..3219454 (+) 567 WP_071980405.1 YqgE/AlgH family protein -
  BM528_RS14315 (BM528_14100) ruvX 3219447..3219902 (+) 456 WP_071980406.1 Holliday junction resolvase RuvX -
  BM528_RS14320 (BM528_14105) - 3219929..3220249 (-) 321 WP_071980407.1 DUF2007 domain-containing protein -
  BM528_RS14325 (BM528_14110) pilU 3220304..3221416 (-) 1113 WP_071980408.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BM528_RS14330 (BM528_14115) pilT 3221475..3222518 (-) 1044 WP_071980409.1 type IV pilus twitching motility protein PilT Machinery gene
  BM528_RS14335 (BM528_14120) - 3222546..3223232 (+) 687 WP_071980410.1 YggS family pyridoxal phosphate-dependent enzyme -
  BM528_RS14340 (BM528_14125) proC 3223408..3224229 (+) 822 WP_071980411.1 pyrroline-5-carboxylate reductase -
  BM528_RS14345 (BM528_14130) - 3224251..3224790 (+) 540 WP_071980412.1 YggT family protein -
  BM528_RS14350 (BM528_14135) - 3224941..3225333 (+) 393 WP_083595403.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41074.05 Da        Isoelectric Point: 6.0503

>NTDB_id=205167 BM528_RS14325 WP_071980408.1 3220304..3221416(-) (pilU) [Alteromonas sp. RW2A1]
MLDSLLQKMIDTNASDLFVTANFPISAKINGKLTPISDSATSEETALALVHEAMTEKQQKEFHESKECNFAIVREGIGRF
RCSAFWQRDQAGMVIRRIVTQIPKADDLGLPRVLKEIIMAKRGLVLFVGGTGTGKSTSLAALIGHRNENSHGHILTIEDP
IEFVHEHKNCVITQREVGIDTQSFDDALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERIM
HLAPKDMHDKLRFDLSLNIRAIVAQQLVPTPDGKGRVAAIEVLLNTPLVRDLIQRNEIGSLKEAMKKGKEQGMQTFDMAL
YDLYKQGRVEMDQALHHADSPNDLRLMIKLDASDNSSLGTLSNVSFDMDD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=205167 BM528_RS14325 WP_071980408.1 3220304..3221416(-) (pilU) [Alteromonas sp. RW2A1]
ATGCTAGACTCTCTTTTACAAAAAATGATTGATACCAATGCCTCAGATTTATTCGTAACGGCAAACTTTCCGATCAGTGC
CAAGATAAACGGAAAGCTTACACCTATTTCCGATAGCGCAACTTCTGAGGAAACTGCTCTCGCTCTGGTTCACGAAGCCA
TGACCGAGAAGCAGCAAAAGGAATTTCACGAATCTAAAGAGTGTAATTTTGCGATTGTAAGAGAGGGCATTGGACGCTTT
CGTTGCTCAGCATTTTGGCAGCGCGATCAAGCCGGCATGGTTATTAGACGTATAGTAACTCAAATTCCTAAAGCGGATGA
TTTAGGGCTTCCCAGAGTTTTAAAAGAAATCATTATGGCAAAACGCGGTTTAGTGCTATTTGTTGGCGGAACGGGTACCG
GTAAATCAACATCATTAGCGGCGTTAATTGGCCATAGAAATGAGAACTCTCACGGTCACATTCTTACCATTGAAGACCCG
ATAGAGTTTGTTCATGAACATAAAAATTGCGTCATCACTCAGCGCGAAGTGGGCATTGATACCCAGTCGTTTGACGACGC
TTTAAAAAGCTCTTTAAGACAGGCCCCTGACGTCATTCTCATTGGGGAAATTCGCTCTATGGAGACCATGGAATACGCTA
TGTCGTTTGCAGATACAGGGCACCTTTGTGTTGCAACTTTGCATGCAAACAACGCTAATCAGGCCATCGAGCGGATAATG
CACTTAGCGCCAAAGGATATGCATGACAAGCTGCGATTCGATTTGAGTTTGAATATTCGCGCCATTGTAGCCCAGCAACT
TGTTCCTACCCCTGATGGTAAGGGCCGCGTAGCCGCTATAGAAGTTTTATTAAATACCCCACTTGTGCGCGACCTTATTC
AGCGAAATGAAATAGGCAGCCTTAAAGAGGCGATGAAGAAAGGGAAAGAGCAGGGAATGCAAACCTTCGATATGGCGCTT
TATGACCTTTATAAACAGGGCCGTGTTGAAATGGATCAGGCTCTGCACCACGCAGACTCGCCTAACGATTTGCGTTTGAT
GATAAAGTTAGACGCCAGCGACAATTCAAGTTTAGGAACACTGTCGAACGTGTCGTTTGACATGGATGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.377

100

0.605
  pilU Acinetobacter baylyi ADP1

57.808

98.649

0.57
  pilU Vibrio cholerae strain A1552

55.435

99.459

0.551
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.319

93.243

0.395

Multiple sequence alignment