Detailed information NTG0020463

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: b2950
• Genome accession: NC_000913
• Coordinates: 3094100..3095080 (-)
• Length: 326 a.a.
• NCBI ID: NP_417425.2
• Uniprot ID: P52052
• Organism: Escherichia coli str. K-12 substr. MG1655
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3089100..3100080

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
b2944	yggI	3089755..3090252 (+)	498	NP_417419.1	protein YggI	-
b2945	endA	3090347..3091054 (+)	708	NP_417420.1	DNA-specific endonuclease I	-
b2946	rsmE	3091134..3091865 (+)	732	NP_417421.4	16S rRNA m(3)U1498 methyltransferase	-
b2947	gshB	3091878..3092828 (+)	951	NP_417422.1	glutathione synthetase	-
b2948	yqgE	3092937..3093500 (+)	564	NP_417423.4	DUF179 domain-containing protein YqgE	-
b2949	yqgF	3093500..3093916 (+)	417	NP_417424.1	ribonuclease H-like domain-containing nuclease	-
b2950	pilT	3094100..3095080 (-)	981	NP_417425.2	Type II/IV secretion system family protein	Machinery gene
b2951	yggS	3095098..3095802 (+)	705	NP_417426.1	PLP homeostasis protein	-
b2952	yggT	3095820..3096386 (+)	567	NP_417427.1	uncharacterized protein YggT	-
b2953	yggU	3096383..3096673 (+)	291	NP_417428.2	DUF167 domain-containing protein YggU	-
b2954	rdgB	3096681..3097274 (+)	594	NP_417429.1	dITP/XTP pyrophosphatase	-
b2955	hemW	3097267..3098403 (+)	1137	NP_417430.1	heme chaperone HemW	-
b2956	yggM	3098558..3099565 (-)	1008	NP_417431.1	DUF1202 domain-containing protein YggM	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35994.16 Da
• Isoelectric Point: 6.0804

>NTDB_id=20463 b2950 NP_417425.2 3094100..3095080(-) (pilT) [Escherichia coli str. K-12 substr. MG1655]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=20463 b2950 NP_417425.2 3094100..3095080(-) (pilT) [Escherichia coli str. K-12 substr. MG1655]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATACGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (61-239)

3D structure

• AlphaFold DB: P52052

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.235	100	0.494
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.235	100	0.494
pilT	Neisseria meningitidis 8013	48.476	100	0.488
pilT	Neisseria gonorrhoeae MS11	48.171	100	0.485
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	46.177	100	0.463
pilT	Legionella pneumophila strain ERS1305867	45.912	97.546	0.448
pilT	Legionella pneumophila strain Lp02	45.912	97.546	0.448
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.824	97.546	0.408
pilU	Vibrio cholerae strain A1552	40.373	98.773	0.399
pilU	Pseudomonas stutzeri DSM 10701	38.554	100	0.393
pilU	Acinetobacter baylyi ADP1	36.061	100	0.365
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362