Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BJN34_RS16585 Genome accession   NZ_CP017757
Coordinates   3596994..3598037 (-) Length   347 a.a.
NCBI ID   WP_078197804.1    Uniprot ID   A0A1U9US20
Organism   Cupriavidus necator strain NH9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3591994..3603037
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJN34_RS16555 (BJN34_16540) - 3592993..3593379 (+) 387 WP_078197798.1 CidA/LrgA family protein -
  BJN34_RS16560 (BJN34_16545) - 3593376..3594101 (+) 726 WP_078197799.1 LrgB family protein -
  BJN34_RS16565 (BJN34_16550) - 3594187..3594558 (+) 372 WP_206363333.1 VOC family protein -
  BJN34_RS16570 (BJN34_16555) - 3594580..3595269 (+) 690 WP_078197801.1 YafY family protein -
  BJN34_RS16575 (BJN34_16560) - 3595276..3595770 (-) 495 WP_078197802.1 glutathione peroxidase -
  BJN34_RS16580 (BJN34_16565) pilU 3595827..3596966 (-) 1140 WP_078197803.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BJN34_RS16585 (BJN34_16570) pilT 3596994..3598037 (-) 1044 WP_078197804.1 type IV pilus twitching motility protein PilT Machinery gene
  BJN34_RS16590 (BJN34_16575) - 3598104..3598799 (+) 696 WP_078197805.1 YggS family pyridoxal phosphate-dependent enzyme -
  BJN34_RS16595 (BJN34_16580) proC 3598857..3599693 (+) 837 WP_078197806.1 pyrroline-5-carboxylate reductase -
  BJN34_RS16600 (BJN34_16585) ubiA 3599765..3600622 (-) 858 WP_078197807.1 4-hydroxybenzoate octaprenyltransferase -
  BJN34_RS16605 (BJN34_16590) - 3600764..3601249 (-) 486 WP_018311510.1 Dps family protein -
  BJN34_RS38005 - 3601425..3601520 (-) 96 Protein_3258 catalase -
  BJN34_RS16610 (BJN34_16595) - 3601686..3602636 (-) 951 WP_078197808.1 LysR substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38349.03 Da        Isoelectric Point: 6.9771

>NTDB_id=202565 BJN34_RS16585 WP_078197804.1 3596994..3598037(-) (pilT) [Cupriavidus necator strain NH9]
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINIASMTHKDVHAMVYDIMSDTQRKAYEERLEIDFSFEIAGLSR
FRVNAYNTQRGAAAVFRTIPSKVLTLEDLRAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNDNDMGHILTVED
PIEFVHSSKKSLINQRELGPHTHSFANALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMTATPAIRHLIRENKIAQMYSMMQTSSGLGMQTLDQC
LSDLIKRGIINYNDARAIAKNPDAFTG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=202565 BJN34_RS16585 WP_078197804.1 3596994..3598037(-) (pilT) [Cupriavidus necator strain NH9]
ATGGACATCGCGCAGCTATTGGCTTTCGCTGTCAAGAACAAGGCGTCTGATCTTCATCTATCCGCGGACATGCCGCCGAT
GGTGCGGATCCACGGCGACATGCGCCGGATCAACATCGCCTCGATGACGCACAAGGATGTCCACGCCATGGTGTACGACA
TCATGAGCGACACCCAGCGCAAGGCCTACGAGGAACGCCTCGAAATCGATTTCTCGTTCGAGATCGCCGGCCTGTCCCGC
TTCCGGGTCAATGCCTACAATACCCAGCGCGGCGCCGCCGCGGTGTTCCGTACCATTCCCTCCAAGGTGCTCACGCTCGA
AGACCTGCGCGCGCCGGCCGTGTTTGCCGACCTGTGCATGAAGCCGCGCGGGCTGGTGCTGGTGACGGGCCCGACCGGCT
CGGGCAAGTCCACCACGCTGGCGGCAATGGTCGACCATCGCAACGACAACGACATGGGCCACATCCTGACGGTGGAGGAC
CCGATCGAATTCGTGCACAGCTCCAAGAAGAGCCTGATCAACCAGCGCGAGCTTGGGCCGCATACGCATTCGTTTGCCAA
CGCGCTGCGCTCGGCGCTGCGTGAAGACCCGGACGTGATCCTGGTGGGCGAGCTGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCCGAGACCGGCCACCTGGTCTTCGGCACGCTGCACACCAGTTCCGCGGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCGCCAGAAGAAAAGGACATGGTGCGCACCATGCTGTCAGAATCGCTCGAGGCGGTGATCTCGCAGAC
GTTGCTGAAGACGCGCGACGGCAATGGCCGCACCGCCGCGCACGAGATCATGACCGCCACGCCCGCGATCCGCCACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTACTCGATGATGCAGACCAGCAGCGGGCTGGGCATGCAGACGCTGGACCAGTGT
CTGTCGGACCTGATCAAGCGCGGCATCATCAACTACAACGACGCGCGCGCCATCGCCAAGAATCCGGACGCGTTCACGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1U9US20

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.594

99.424

0.712
  pilT Acinetobacter baumannii D1279779

71.594

99.424

0.712
  pilT Acinetobacter baumannii strain A118

71.594

99.424

0.712
  pilT Acinetobacter baylyi ADP1

69.855

99.424

0.695
  pilT Pseudomonas stutzeri DSM 10701

69.855

99.424

0.695
  pilT Pseudomonas aeruginosa PAK

69.275

99.424

0.689
  pilT Legionella pneumophila strain Lp02

67.246

99.424

0.669
  pilT Legionella pneumophila strain ERS1305867

67.246

99.424

0.669
  pilT Neisseria gonorrhoeae MS11

66.474

99.712

0.663
  pilT Neisseria meningitidis 8013

66.474

99.712

0.663
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.875

97.118

0.64
  pilT Vibrio cholerae strain A1552

65.875

97.118

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

99.424

0.49
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395
  pilU Acinetobacter baylyi ADP1

39.826

99.135

0.395
  pilU Vibrio cholerae strain A1552

40

96.542

0.386

Multiple sequence alignment