Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BJN34_RS16580 Genome accession   NZ_CP017757
Coordinates   3595827..3596966 (-) Length   379 a.a.
NCBI ID   WP_078197803.1    Uniprot ID   A0A1U9US70
Organism   Cupriavidus necator strain NH9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3590827..3601966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJN34_RS16550 (BJN34_16535) glcF 3591661..3592914 (+) 1254 WP_078197797.1 glycolate oxidase subunit GlcF -
  BJN34_RS16555 (BJN34_16540) - 3592993..3593379 (+) 387 WP_078197798.1 CidA/LrgA family protein -
  BJN34_RS16560 (BJN34_16545) - 3593376..3594101 (+) 726 WP_078197799.1 LrgB family protein -
  BJN34_RS16565 (BJN34_16550) - 3594187..3594558 (+) 372 WP_206363333.1 VOC family protein -
  BJN34_RS16570 (BJN34_16555) - 3594580..3595269 (+) 690 WP_078197801.1 YafY family protein -
  BJN34_RS16575 (BJN34_16560) - 3595276..3595770 (-) 495 WP_078197802.1 glutathione peroxidase -
  BJN34_RS16580 (BJN34_16565) pilU 3595827..3596966 (-) 1140 WP_078197803.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BJN34_RS16585 (BJN34_16570) pilT 3596994..3598037 (-) 1044 WP_078197804.1 type IV pilus twitching motility protein PilT Machinery gene
  BJN34_RS16590 (BJN34_16575) - 3598104..3598799 (+) 696 WP_078197805.1 YggS family pyridoxal phosphate-dependent enzyme -
  BJN34_RS16595 (BJN34_16580) proC 3598857..3599693 (+) 837 WP_078197806.1 pyrroline-5-carboxylate reductase -
  BJN34_RS16600 (BJN34_16585) ubiA 3599765..3600622 (-) 858 WP_078197807.1 4-hydroxybenzoate octaprenyltransferase -
  BJN34_RS16605 (BJN34_16590) - 3600764..3601249 (-) 486 WP_018311510.1 Dps family protein -
  BJN34_RS38005 - 3601425..3601520 (-) 96 Protein_3258 catalase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42082.33 Da        Isoelectric Point: 6.5044

>NTDB_id=202564 BJN34_RS16580 WP_078197803.1 3595827..3596966(-) (pilU) [Cupriavidus necator strain NH9]
MLDRESAAKYINDLLELMVSNRGSDLFITSDFPPAIKVDGKLTPVSQQPLNPTQALGLVRSVMNERQVQEFDTSRECNFA
ITAPKAGRFRVSAFIQQGKAGMVVRTINTRIPSVADLDLPPTLHDVVMAKRGLVIVTGATGSGKSTTLAAMLDHRNAHSY
GHIITIEDPIEYVHAHQNCIVTQREVGIDTESWHVALKNTLRQAPDVILIGEIRDRETMEYAMQYAETGHLCLATLHANN
ANQAIDRVVNFFPEEKRQQLLIDLSLNLKAMISQRLLPRAGKKGRVPAVEIMIGTPLVADLIFKGEIHELKEVIKKSREQ
GMISFDQALFDLYEEGKITYEDALRNADSLNDLRLMIKLHSASAKDTDLGAGTEHLNVI

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=202564 BJN34_RS16580 WP_078197803.1 3595827..3596966(-) (pilU) [Cupriavidus necator strain NH9]
ATGCTCGATCGCGAATCCGCCGCCAAGTACATCAACGACCTGCTGGAGCTGATGGTCAGCAACCGCGGCTCGGACCTGTT
CATCACCTCCGACTTCCCGCCGGCGATCAAGGTCGACGGCAAGCTCACGCCGGTGTCGCAGCAGCCGCTGAATCCCACGC
AGGCACTTGGTCTGGTGCGCTCGGTGATGAACGAGCGCCAGGTGCAGGAGTTCGATACCAGCCGCGAATGCAATTTCGCG
ATCACCGCGCCCAAGGCCGGGCGCTTCCGCGTGTCGGCCTTTATCCAGCAAGGCAAGGCCGGCATGGTGGTGCGCACCAT
CAATACGCGCATCCCTTCGGTGGCCGACCTGGACCTGCCGCCGACGCTGCATGACGTGGTCATGGCCAAACGCGGGCTGG
TGATCGTGACCGGCGCCACGGGCTCGGGCAAGTCGACCACGCTGGCGGCGATGCTCGATCACCGCAACGCGCATTCCTAC
GGCCATATCATCACCATCGAGGACCCGATCGAGTACGTGCACGCGCACCAGAACTGCATCGTCACGCAGCGCGAGGTGGG
TATCGACACCGAGTCCTGGCACGTGGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATCGGCGAAATCC
GCGACCGCGAGACCATGGAGTACGCGATGCAGTACGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAAC
GCCAACCAGGCGATCGACCGCGTGGTCAACTTCTTCCCCGAGGAAAAGCGCCAGCAGCTGCTGATCGACCTGTCGCTGAA
CCTGAAGGCGATGATCTCGCAGCGCCTGCTGCCGCGCGCCGGCAAAAAGGGGCGCGTGCCGGCGGTGGAGATCATGATCG
GCACGCCGCTGGTGGCCGACCTGATCTTCAAGGGCGAGATCCACGAGCTCAAGGAAGTCATCAAGAAGTCGCGCGAGCAG
GGCATGATCTCGTTCGACCAGGCGCTGTTCGACCTGTACGAGGAAGGCAAGATCACCTACGAGGACGCGCTGCGCAACGC
CGACTCGCTCAACGACCTGCGCCTGATGATCAAGCTGCACAGCGCGAGCGCCAAGGACACCGACCTGGGTGCCGGCACCG
AGCACCTCAACGTCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1U9US70

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.571

92.348

0.578
  pilU Acinetobacter baylyi ADP1

57.103

94.723

0.541
  pilU Vibrio cholerae strain A1552

53.994

95.778

0.517
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

88.918

0.401
  pilT Pseudomonas aeruginosa PAK

43.027

88.918

0.383
  pilT Pseudomonas stutzeri DSM 10701

43.027

88.918

0.383
  pilT Legionella pneumophila strain Lp02

43.243

87.863

0.38
  pilT Legionella pneumophila strain ERS1305867

43.243

87.863

0.38
  pilT Acinetobacter nosocomialis M2

42.943

87.863

0.377
  pilT Acinetobacter baumannii D1279779

42.943

87.863

0.377
  pilT Acinetobacter baumannii strain A118

42.943

87.863

0.377
  pilT Acinetobacter baylyi ADP1

42.643

87.863

0.375
  pilT Neisseria meningitidis 8013

40.294

89.71

0.361

Multiple sequence alignment