Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BKK81_RS16910 Genome accession   NZ_CP017751
Coordinates   3828044..3829090 (-) Length   348 a.a.
NCBI ID   WP_071015324.1    Uniprot ID   A0A1D9GZF9
Organism   Cupriavidus sp. USMAHM13 isolate pure     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3823044..3834090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKK81_RS16880 (BKK81_16880) - 3823888..3824253 (+) 366 WP_071015309.1 CidA/LrgA family protein -
  BKK81_RS16885 (BKK81_16885) - 3824256..3824978 (+) 723 WP_071015311.1 LrgB family protein -
  BKK81_RS16890 (BKK81_16890) - 3825064..3825444 (+) 381 WP_156811314.1 VOC family protein -
  BKK81_RS16895 (BKK81_16895) - 3825456..3826145 (+) 690 WP_071015316.1 YafY family protein -
  BKK81_RS16900 (BKK81_16900) - 3826246..3826746 (-) 501 WP_071015318.1 glutathione peroxidase -
  BKK81_RS16905 (BKK81_16905) pilU 3826823..3827962 (-) 1140 WP_071015321.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BKK81_RS16910 (BKK81_16910) pilT 3828044..3829090 (-) 1047 WP_071015324.1 type IV pilus twitching motility protein PilT Machinery gene
  BKK81_RS16915 (BKK81_16915) - 3829257..3829964 (+) 708 WP_071015327.1 YggS family pyridoxal phosphate-dependent enzyme -
  BKK81_RS16920 (BKK81_16920) proC 3830027..3830863 (+) 837 WP_071015330.1 pyrroline-5-carboxylate reductase -
  BKK81_RS16925 (BKK81_16925) ubiA 3830981..3831838 (-) 858 WP_071015334.1 4-hydroxybenzoate octaprenyltransferase -
  BKK81_RS16930 (BKK81_16930) - 3832051..3832536 (-) 486 WP_071015337.1 Dps family protein -
  BKK81_RS16935 (BKK81_16935) - 3832651..3832833 (-) 183 WP_071015339.1 hypothetical protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38379.14 Da        Isoelectric Point: 7.0872

>NTDB_id=202523 BKK81_RS16910 WP_071015324.1 3828044..3829090(-) (pilT) [Cupriavidus sp. USMAHM13 isolate pure]
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVAAMAHKDVHAMVYDIMSDTQRKAYEERLEVDFSFEIAGLSR
FRVNAYNTQRGAAAVFRTIPSKVLTLEDLRAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNEHDMGHILTVED
PIEFVHHSKKSLINQRELGPHTHSFANALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAVHEIMIATPAIRHLIRENKIAQMYSMMQTSSGLGMQTLDQG
LTDLIKRGVIHYGDARAIAKNPDAFTGG

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=202523 BKK81_RS16910 WP_071015324.1 3828044..3829090(-) (pilT) [Cupriavidus sp. USMAHM13 isolate pure]
ATGGACATCGCGCAGCTATTGGCTTTCGCCGTCAAGAACAAGGCCTCCGATCTCCATCTGTCGGCCGACATGCCGCCGAT
GGTGCGGATCCACGGCGACATGCGCCGCATCAATGTCGCCGCGATGGCGCACAAGGATGTGCACGCCATGGTGTACGACA
TCATGAGCGACACCCAGCGCAAGGCCTATGAAGAGCGCCTCGAGGTCGATTTCTCCTTCGAGATCGCCGGCCTGTCGCGT
TTCCGCGTCAACGCCTACAACACGCAGCGCGGCGCTGCCGCTGTGTTCCGCACGATTCCGTCCAAGGTGCTCACGCTGGA
GGACCTGCGCGCGCCCGCCGTGTTCGCCGACCTGTGCATGAAGCCACGCGGCCTGGTGCTGGTCACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTCGCGGCCATGGTGGACCACCGCAACGAGCACGACATGGGCCACATCCTGACGGTGGAAGAC
CCGATCGAGTTCGTGCACCACTCGAAGAAGAGCCTGATCAACCAGCGCGAACTCGGGCCGCACACCCATTCCTTCGCCAA
TGCGCTGCGCTCCGCGCTGCGCGAGGATCCGGACGTGATCCTGGTCGGCGAGCTGCGCGACCTGGAGACCATCCGGCTGG
CCTTGACCGCCGCCGAGACCGGCCACCTGGTCTTCGGCACGCTGCATACCAGCTCCGCGGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCGCCGGAGGAGAAGGACATGGTGCGCACCATGCTGTCGGAATCGCTGGAGGCGGTGATCTCGCAGAC
GCTGCTGAAGACGCGCGACGGCAATGGCCGCACCGCGGTGCACGAGATCATGATCGCCACACCCGCCATCCGCCACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTATTCGATGATGCAGACCAGCAGCGGGCTGGGCATGCAGACGCTGGACCAGGGG
CTGACGGACCTGATCAAGCGCGGCGTGATCCACTACGGCGACGCGCGCGCCATCGCCAAGAATCCCGACGCCTTCACCGG
CGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D9GZF9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

72.174

99.138

0.716
  pilT Acinetobacter nosocomialis M2

72.174

99.138

0.716
  pilT Acinetobacter baumannii strain A118

72.174

99.138

0.716
  pilT Pseudomonas stutzeri DSM 10701

69.855

99.138

0.693
  pilT Acinetobacter baylyi ADP1

69.855

99.138

0.693
  pilT Pseudomonas aeruginosa PAK

68.986

99.138

0.684
  pilT Neisseria gonorrhoeae MS11

67.341

99.425

0.67
  pilT Neisseria meningitidis 8013

67.341

99.425

0.67
  pilT Legionella pneumophila strain Lp02

66.957

99.138

0.664
  pilT Legionella pneumophila strain ERS1305867

66.957

99.138

0.664
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.182

94.828

0.647
  pilT Vibrio cholerae strain A1552

68.182

94.828

0.647
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

99.138

0.486
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.264

0.397
  pilU Acinetobacter baylyi ADP1

40.116

98.851

0.397
  pilU Vibrio cholerae strain A1552

40.597

96.264

0.391

Multiple sequence alignment