Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BKK81_RS16905 Genome accession   NZ_CP017751
Coordinates   3826823..3827962 (-) Length   379 a.a.
NCBI ID   WP_071015321.1    Uniprot ID   -
Organism   Cupriavidus sp. USMAHM13 isolate pure     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3821823..3832962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKK81_RS16875 (BKK81_16875) glcF 3822389..3823633 (+) 1245 WP_071015306.1 glycolate oxidase subunit GlcF -
  BKK81_RS16880 (BKK81_16880) - 3823888..3824253 (+) 366 WP_071015309.1 CidA/LrgA family protein -
  BKK81_RS16885 (BKK81_16885) - 3824256..3824978 (+) 723 WP_071015311.1 LrgB family protein -
  BKK81_RS16890 (BKK81_16890) - 3825064..3825444 (+) 381 WP_156811314.1 VOC family protein -
  BKK81_RS16895 (BKK81_16895) - 3825456..3826145 (+) 690 WP_071015316.1 YafY family protein -
  BKK81_RS16900 (BKK81_16900) - 3826246..3826746 (-) 501 WP_071015318.1 glutathione peroxidase -
  BKK81_RS16905 (BKK81_16905) pilU 3826823..3827962 (-) 1140 WP_071015321.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BKK81_RS16910 (BKK81_16910) pilT 3828044..3829090 (-) 1047 WP_071015324.1 type IV pilus twitching motility protein PilT Machinery gene
  BKK81_RS16915 (BKK81_16915) - 3829257..3829964 (+) 708 WP_071015327.1 YggS family pyridoxal phosphate-dependent enzyme -
  BKK81_RS16920 (BKK81_16920) proC 3830027..3830863 (+) 837 WP_071015330.1 pyrroline-5-carboxylate reductase -
  BKK81_RS16925 (BKK81_16925) ubiA 3830981..3831838 (-) 858 WP_071015334.1 4-hydroxybenzoate octaprenyltransferase -
  BKK81_RS16930 (BKK81_16930) - 3832051..3832536 (-) 486 WP_071015337.1 Dps family protein -
  BKK81_RS16935 (BKK81_16935) - 3832651..3832833 (-) 183 WP_071015339.1 hypothetical protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42287.59 Da        Isoelectric Point: 6.4431

>NTDB_id=202522 BKK81_RS16905 WP_071015321.1 3826823..3827962(-) (pilU) [Cupriavidus sp. USMAHM13 isolate pure]
MLDRESAAKYIHELLVLMVGNRGSDLFITADFPPAIKVDGKITPVSQQPLNVTQALGLVRAIMNERQIAEFDDSHECNFA
LSVPNAGRFRASAFIQQGKAGMVLRTINTRIPTVAELDLPATLHEIVMSKRGLVIVTGATGSGKSTTLAAMLDHRNAQSY
GHIITIEDPIEYVHAHQNCIVTQREVGIDTESWHVALKNTLRQAPDVILIGEIRDRETMEYAMQYAETGHLCLATLHANN
ANQAIDRVVNFFPEEKRQQLLIDLSLNLKAMISQRLLPRKGRKGRVPAVEIMIGTPLVADLIFKGEIHELKEVIKKSREQ
GMISFDQALFDLYEEDKISYEDALRNADSLNDLRLMIKLHSTRNRDGDLAAGTEHLNVI

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=202522 BKK81_RS16905 WP_071015321.1 3826823..3827962(-) (pilU) [Cupriavidus sp. USMAHM13 isolate pure]
ATGCTCGATCGCGAATCCGCCGCCAAGTACATCCACGAGCTGCTGGTGCTGATGGTAGGCAACCGCGGCTCGGACCTGTT
CATCACCGCCGATTTTCCGCCGGCGATCAAGGTGGACGGCAAGATCACGCCGGTCTCGCAGCAGCCGCTCAACGTCACCC
AGGCGCTCGGGCTGGTGCGGGCCATCATGAACGAGCGGCAGATCGCCGAGTTCGACGACAGCCACGAATGCAACTTCGCC
CTGTCGGTGCCCAACGCCGGGCGCTTCCGCGCTTCGGCCTTCATCCAGCAGGGCAAGGCCGGCATGGTGCTGCGGACCAT
CAATACGCGCATCCCCACCGTGGCCGAACTCGACCTGCCGGCCACGCTGCACGAGATCGTGATGTCCAAGCGCGGCCTGG
TGATCGTCACCGGCGCCACCGGCTCGGGCAAGTCGACCACGCTGGCGGCCATGCTGGATCACCGCAACGCGCAGTCCTAC
GGCCACATCATCACCATCGAAGACCCGATCGAGTACGTGCACGCCCACCAGAACTGCATCGTCACGCAGCGCGAGGTGGG
CATCGATACCGAAAGCTGGCACGTGGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATCGGTGAGATCC
GCGACCGCGAGACCATGGAGTACGCGATGCAGTACGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAAC
GCGAACCAGGCCATCGACCGCGTGGTGAACTTCTTTCCCGAGGAAAAGCGCCAGCAGTTGCTGATCGACCTGTCGCTGAA
CCTCAAGGCGATGATTTCGCAGCGCCTGCTGCCGCGCAAGGGGCGCAAGGGCCGGGTGCCGGCGGTGGAGATCATGATCG
GCACGCCGCTGGTGGCCGACCTGATCTTCAAGGGCGAGATCCACGAGCTCAAGGAGGTCATCAAGAAATCGCGCGAGCAG
GGCATGATCTCTTTCGACCAGGCGCTGTTCGACCTGTACGAGGAAGACAAGATCAGCTACGAGGATGCATTGCGCAATGC
CGACTCGCTCAACGACCTGCGGCTGATGATCAAGCTGCACAGCACCAGGAACCGCGACGGCGACCTGGCCGCCGGCACCG
AGCACCTGAACGTGATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.823

92.612

0.573
  pilU Acinetobacter baylyi ADP1

56.267

94.723

0.533
  pilU Vibrio cholerae strain A1552

55.618

93.931

0.522
  pilT Pseudomonas aeruginosa PAK

44.214

88.918

0.393
  pilT Acinetobacter baylyi ADP1

43.658

89.446

0.391
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.917

88.918

0.391
  pilT Acinetobacter nosocomialis M2

44.144

87.863

0.388
  pilT Acinetobacter baumannii D1279779

44.144

87.863

0.388
  pilT Acinetobacter baumannii strain A118

44.144

87.863

0.388
  pilT Pseudomonas stutzeri DSM 10701

43.62

88.918

0.388
  pilT Legionella pneumophila strain Lp02

43.544

87.863

0.383
  pilT Legionella pneumophila strain ERS1305867

43.544

87.863

0.383

Multiple sequence alignment