Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BI364_RS01350 Genome accession   NZ_CP017415
Coordinates   272436..273470 (+) Length   344 a.a.
NCBI ID   WP_070077222.1    Uniprot ID   -
Organism   Acidihalobacter yilgarnensis strain F5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 267436..278470
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BI364_RS01325 (BI364_01325) - 267935..269758 (-) 1824 WP_083251087.1 chloride channel protein -
  BI364_RS01330 (BI364_01330) - 269885..270196 (-) 312 WP_070077219.1 DUF167 domain-containing protein -
  BI364_RS01335 (BI364_01335) - 270199..270753 (-) 555 WP_070077220.1 YggT family protein -
  BI364_RS01340 (BI364_01340) proC 270755..271570 (-) 816 WP_070079809.1 pyrroline-5-carboxylate reductase -
  BI364_RS01345 (BI364_01345) - 271641..272324 (-) 684 WP_070077221.1 YggS family pyridoxal phosphate-dependent enzyme -
  BI364_RS01350 (BI364_01350) pilT 272436..273470 (+) 1035 WP_070077222.1 type IV pilus twitching motility protein PilT Machinery gene
  BI364_RS01355 (BI364_01355) pilU 273506..274651 (+) 1146 WP_070077223.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BI364_RS01360 (BI364_01360) - 274680..275837 (+) 1158 WP_070077224.1 PilT/PilU family type 4a pilus ATPase -
  BI364_RS01365 (BI364_01365) - 275847..276731 (-) 885 WP_070077225.1 dihydroorotate dehydrogenase electron transfer subunit -
  BI364_RS01370 (BI364_01370) - 276728..278020 (-) 1293 WP_070077226.1 dihydroorotase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37835.32 Da        Isoelectric Point: 6.8395

>NTDB_id=197981 BI364_RS01350 WP_070077222.1 272436..273470(+) (pilT) [Acidihalobacter yilgarnensis strain F5]
MDITQLLAFTVKNGASDLHLSAQLPPMIRVDGDIRRINVDALDHSTVHDMIYDIMNDRQRKDYEERLETDFSFEIQGLAR
FRVNAFNQDRGAAAVFRTIPSKVLTLEQLGAPSILQKIAGYPRGIVLVTGPTGSGKSTTLAAMVDFKNDSEYGHILTVED
PIEFVHQSKKCLVNQREVHRDTLGFNEALRSALREDPDTILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPSGEKDMVRAMLSESLRAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREGKIAQMNSAIQTGHGMGMQTLDQSL
KELLSRGLASKEDARRKAVSPDAL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=197981 BI364_RS01350 WP_070077222.1 272436..273470(+) (pilT) [Acidihalobacter yilgarnensis strain F5]
ATGGATATCACGCAGTTGCTGGCCTTTACCGTCAAGAATGGGGCCTCCGACCTGCATCTTTCCGCGCAATTGCCGCCGAT
GATTCGTGTCGATGGCGATATTCGCAGGATCAATGTCGACGCACTGGATCACAGTACGGTACACGACATGATCTACGACA
TCATGAACGATCGTCAGCGTAAGGACTACGAAGAGCGCCTGGAGACCGATTTTTCTTTCGAGATTCAGGGCTTGGCGCGT
TTCCGTGTGAATGCCTTCAACCAGGATCGTGGTGCTGCGGCGGTATTTCGTACCATTCCCTCCAAGGTGCTGACCCTGGA
GCAGCTCGGTGCGCCGTCGATACTGCAGAAGATCGCCGGATACCCGCGTGGCATCGTGCTGGTGACCGGTCCGACCGGTT
CCGGCAAGTCGACTACGCTTGCGGCGATGGTCGATTTCAAGAACGACAGTGAATACGGGCATATCCTGACGGTGGAGGAC
CCGATCGAATTTGTGCACCAAAGCAAGAAGTGTCTGGTCAACCAGCGTGAGGTGCACCGCGATACCCTCGGCTTCAACGA
GGCGCTGCGTTCGGCGCTGCGCGAGGATCCCGACACCATCCTGGTGGGCGAGCTGCGCGATCTTGAAACGATTCGACTGG
CCTTGACGGCCGCCGAAACTGGGCATTTAGTGTTCGGCACCTTGCATACCAGTTCCGCCGCCAAGACCATCGACCGTGTG
GTCGACGTATTTCCGTCGGGTGAAAAGGACATGGTGCGCGCGATGCTTTCCGAATCCCTGCGCGCGGTCATCTCGCAGAC
GCTGCTCAAGAAAATTGGCGGCGGTCGCGTGGCCGCCCACGAGATCATGATCGGCACCCCCGCGATCCGCAATCTGATCC
GCGAAGGCAAGATCGCGCAAATGAATTCCGCCATTCAGACGGGGCACGGCATGGGCATGCAGACCCTTGATCAGTCGCTC
AAGGAGCTGCTGTCGCGCGGTCTGGCCAGCAAGGAAGATGCCCGCCGCAAGGCCGTCAGCCCGGACGCCTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.07

100

0.791
  pilT Acinetobacter baumannii D1279779

78.779

100

0.788
  pilT Acinetobacter baumannii strain A118

78.779

100

0.788
  pilT Pseudomonas stutzeri DSM 10701

78.363

99.419

0.779
  pilT Pseudomonas aeruginosa PAK

78.07

99.419

0.776
  pilT Acinetobacter baylyi ADP1

77.616

100

0.776
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

67.826

100

0.68
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.585

99.419

0.503
  pilU Pseudomonas stutzeri DSM 10701

40.29

100

0.404
  pilU Vibrio cholerae strain A1552

40.29

100

0.404
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395

Multiple sequence alignment