Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BI364_RS01355 Genome accession   NZ_CP017415
Coordinates   273506..274651 (+) Length   381 a.a.
NCBI ID   WP_070077223.1    Uniprot ID   A0A1D8IK30
Organism   Acidihalobacter yilgarnensis strain F5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 268506..279651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BI364_RS01330 (BI364_01330) - 269885..270196 (-) 312 WP_070077219.1 DUF167 domain-containing protein -
  BI364_RS01335 (BI364_01335) - 270199..270753 (-) 555 WP_070077220.1 YggT family protein -
  BI364_RS01340 (BI364_01340) proC 270755..271570 (-) 816 WP_070079809.1 pyrroline-5-carboxylate reductase -
  BI364_RS01345 (BI364_01345) - 271641..272324 (-) 684 WP_070077221.1 YggS family pyridoxal phosphate-dependent enzyme -
  BI364_RS01350 (BI364_01350) pilT 272436..273470 (+) 1035 WP_070077222.1 type IV pilus twitching motility protein PilT Machinery gene
  BI364_RS01355 (BI364_01355) pilU 273506..274651 (+) 1146 WP_070077223.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BI364_RS01360 (BI364_01360) - 274680..275837 (+) 1158 WP_070077224.1 PilT/PilU family type 4a pilus ATPase -
  BI364_RS01365 (BI364_01365) - 275847..276731 (-) 885 WP_070077225.1 dihydroorotate dehydrogenase electron transfer subunit -
  BI364_RS01370 (BI364_01370) - 276728..278020 (-) 1293 WP_070077226.1 dihydroorotase -
  BI364_RS01375 (BI364_01375) - 278017..279018 (-) 1002 WP_070077227.1 aspartate carbamoyltransferase catalytic subunit -
  BI364_RS01380 (BI364_01380) pyrR 279015..279542 (-) 528 WP_070077228.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42567.71 Da        Isoelectric Point: 6.3494

>NTDB_id=197982 BI364_RS01355 WP_070077223.1 273506..274651(+) (pilU) [Acidihalobacter yilgarnensis strain F5]
MERDKAIRFMHDLLKSLLSKRGSDLFITVGAPPSIKVDGRMTPLTNQPLSQAHTQALVRSVMNDKQAGDFEREMECNFAI
GLPGVARFRVNAFTQRGSAGMVLRIINTEIPSFEDLNLPPILRDIAMTRRGMVIFVGGTGSGKSSSLAAMLGYRNHNSYG
HIVTIEDPIEYVHSHGNCLVTQREVGVDTDSYEIALKNTLRQAPDVILIGEIRERETMDYANAFAETGHLCLSTLHANNT
NQALDRIINFFSEERRHQLLMDLSLNLKAVISQRLIPRADGEGRVPAVEVLINSPLMADLIFKGKVHEMKELIAKSNEMG
MQTFDQSLFDLFERGLITYEDALRNADSVNDLRLRIKLESNRARGKDLLADVPQFTVAESD

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=197982 BI364_RS01355 WP_070077223.1 273506..274651(+) (pilU) [Acidihalobacter yilgarnensis strain F5]
ATGGAACGCGACAAGGCCATACGATTCATGCACGACTTGCTGAAGTCTTTGCTCAGCAAGCGTGGCTCGGATCTGTTCAT
CACCGTGGGTGCCCCCCCCTCGATCAAGGTCGACGGCAGGATGACGCCGTTGACCAATCAGCCCCTATCGCAGGCGCATA
CCCAGGCGTTGGTGCGCTCGGTGATGAATGACAAGCAGGCGGGTGACTTCGAGCGCGAGATGGAGTGCAACTTCGCCATC
GGTTTGCCGGGTGTGGCGCGTTTTCGTGTCAACGCCTTCACCCAACGTGGTAGCGCAGGCATGGTGTTGCGCATCATCAA
CACCGAAATTCCGAGCTTTGAGGATCTGAATCTGCCGCCGATTCTGCGCGACATCGCGATGACGCGACGCGGTATGGTGA
TCTTCGTCGGCGGCACCGGTTCGGGTAAATCCAGCTCGCTCGCGGCCATGCTCGGCTACCGCAATCACAACAGCTACGGG
CATATCGTCACCATCGAGGACCCAATCGAATACGTCCATTCCCACGGCAACTGCCTGGTGACCCAGCGCGAGGTGGGCGT
CGATACCGATTCGTACGAAATCGCCCTGAAGAACACACTGCGCCAGGCGCCAGACGTGATTCTGATCGGCGAAATCCGTG
AGCGCGAGACCATGGACTACGCCAATGCCTTCGCGGAAACAGGGCATCTATGCCTGTCGACCCTGCATGCGAACAACACC
AACCAGGCCCTTGATCGCATCATCAACTTCTTCAGCGAGGAGCGTCGCCACCAGCTGCTCATGGACCTCTCGCTCAACCT
CAAGGCGGTGATCTCGCAGCGACTGATCCCCAGGGCCGATGGCGAAGGACGAGTGCCGGCAGTGGAGGTGCTGATCAACA
GTCCGTTGATGGCGGATCTGATCTTCAAGGGCAAAGTGCACGAAATGAAGGAGTTGATCGCCAAATCCAACGAGATGGGC
ATGCAGACCTTCGATCAATCCTTGTTTGATCTCTTCGAGCGCGGGTTGATTACCTATGAGGATGCGTTGCGCAACGCGGA
TTCGGTGAACGACCTGCGTCTGCGCATCAAGCTCGAGAGCAACCGCGCGCGCGGCAAGGATCTGCTCGCCGACGTGCCGC
AATTCACCGTCGCCGAAAGCGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D8IK30

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.368

91.339

0.588
  pilU Acinetobacter baylyi ADP1

59.322

92.913

0.551
  pilU Vibrio cholerae strain A1552

53.444

95.276

0.509
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.179

87.927

0.388
  pilT Acinetobacter baumannii D1279779

44.411

86.877

0.386
  pilT Acinetobacter nosocomialis M2

44.411

86.877

0.386
  pilT Acinetobacter baumannii strain A118

44.411

86.877

0.386
  pilT Pseudomonas stutzeri DSM 10701

44.012

87.664

0.386
  pilT Pseudomonas aeruginosa PAK

44.012

87.664

0.386
  pilT Neisseria gonorrhoeae MS11

43.195

88.714

0.383
  pilT Acinetobacter baylyi ADP1

43.323

88.451

0.383
  pilT Neisseria meningitidis 8013

42.899

88.714

0.381
  pilT Legionella pneumophila strain Lp02

41.994

86.877

0.365
  pilT Legionella pneumophila strain ERS1305867

41.994

86.877

0.365

Multiple sequence alignment