Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MTJW_RS08000 Genome accession   NZ_CP017237
Coordinates   1571141..1572160 (-) Length   339 a.a.
NCBI ID   WP_069589960.1    Uniprot ID   A0AAC9MVB8
Organism   Moorella thermoacetica strain DSM 103284     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1566141..1577160
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTJW_RS07970 (MTJW_15970) - 1566602..1567102 (-) 501 WP_071541850.1 hypothetical protein -
  MTJW_RS07975 (MTJW_15980) - 1567099..1567497 (-) 399 WP_071541851.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MTJW_RS07980 (MTJW_15990) - 1567494..1568000 (-) 507 WP_071541852.1 type II secretion system protein -
  MTJW_RS07985 (MTJW_16000) - 1568047..1568790 (-) 744 WP_071541853.1 A24 family peptidase -
  MTJW_RS07990 (MTJW_16010) - 1568955..1569491 (-) 537 WP_071541854.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MTJW_RS07995 (MTJW_16020) - 1569737..1570945 (-) 1209 WP_071542514.1 type II secretion system F family protein -
  MTJW_RS08000 (MTJW_16030) pilT 1571141..1572160 (-) 1020 WP_069589960.1 type IV pilus twitching motility protein PilT Machinery gene
  MTJW_RS08005 (MTJW_16040) pilF 1572174..1573835 (-) 1662 WP_071541855.1 GspE/PulE family protein Machinery gene
  MTJW_RS08010 (MTJW_16050) aroB 1573841..1574935 (-) 1095 WP_071541856.1 3-dehydroquinate synthase -
  MTJW_RS08015 (MTJW_16060) - 1574914..1575435 (-) 522 WP_071541857.1 shikimate kinase -
  MTJW_RS08020 (MTJW_16070) aroC 1575489..1576640 (-) 1152 WP_071541858.1 chorismate synthase -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 37061.70 Da        Isoelectric Point: 6.7282

>NTDB_id=196806 MTJW_RS08000 WP_069589960.1 1571141..1572160(-) (pilT) [Moorella thermoacetica strain DSM 103284]
MLDIETILVAAAAAGASDVHISVGLPPVFRVHGELQVQRQWDPLDLEMTARLVRPIVGDKWEIFQEQGEIDLAYSLPGVS
RFRVNVFHQRGSVGAAIRLIPREIPSLESLGLPPVVAELAGRQHGLILVTGPTGSGKSTTLAAMVDKINRERSCHIITLE
DPIEYLHQHRRSIVNQREVGSDTRSFASALRAALRQDPDVILVGEMRDLETIATAITAAETGHLVLATLHTSSAVQSVDR
IIDVFPPHQQGQVRIQLADTLEGVITQQLLPRADRKGRVAAVEVLIATPAVKNLIREGKTHQIVSTMQTGARYGMQTMEM
ALRQLITRGVIFEEGLNIG

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=196806 MTJW_RS08000 WP_069589960.1 1571141..1572160(-) (pilT) [Moorella thermoacetica strain DSM 103284]
ATGCTCGATATCGAAACAATCCTGGTAGCGGCTGCCGCCGCCGGTGCCTCCGACGTCCATATCTCTGTTGGGCTACCGCC
GGTTTTCCGGGTGCACGGCGAACTCCAGGTCCAGCGCCAGTGGGACCCCCTGGATTTGGAGATGACAGCCCGCCTGGTAC
GGCCTATAGTGGGCGATAAGTGGGAGATATTTCAGGAGCAAGGTGAAATCGATCTGGCTTACTCCCTGCCCGGTGTAAGC
CGTTTCCGGGTCAATGTCTTTCACCAACGGGGGAGCGTCGGGGCGGCCATCCGCCTTATCCCCAGGGAGATCCCCAGCCT
GGAGTCCCTGGGTTTACCGCCGGTGGTGGCCGAACTGGCGGGCAGACAGCACGGCCTGATCCTGGTGACGGGGCCGACGG
GCAGCGGTAAATCCACCACCCTGGCGGCCATGGTGGATAAAATCAACCGGGAGCGGAGCTGTCACATCATCACCCTGGAA
GACCCCATTGAGTACTTGCACCAGCACCGCCGCAGTATAGTCAACCAGCGGGAAGTGGGTTCTGATACCAGGTCCTTTGC
TAGTGCCCTGCGGGCCGCCCTGCGTCAGGACCCCGACGTTATCCTGGTCGGGGAGATGCGCGACCTGGAAACCATCGCCA
CAGCCATTACGGCTGCCGAAACAGGTCACCTGGTCCTGGCGACTTTGCACACCAGCAGTGCCGTCCAGAGTGTGGATCGG
ATCATTGACGTTTTCCCGCCCCACCAGCAGGGGCAGGTACGGATCCAGCTTGCCGACACCCTGGAAGGGGTGATTACCCA
GCAGCTTTTACCGCGGGCTGACAGGAAGGGCCGGGTGGCGGCTGTAGAAGTGTTGATAGCCACCCCGGCAGTGAAGAATC
TCATCCGCGAGGGTAAAACCCATCAAATCGTTTCCACTATGCAGACCGGAGCCCGCTACGGGATGCAGACCATGGAGATG
GCGCTGCGGCAACTGATCACCCGGGGAGTGATTTTTGAGGAAGGTTTAAATATTGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(78-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

56.38

99.41

0.56
  pilT Vibrio cholerae strain A1552

51.807

97.935

0.507
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.807

97.935

0.507
  pilT Acinetobacter baumannii D1279779

51.205

97.935

0.501
  pilT Acinetobacter baumannii strain A118

51.205

97.935

0.501
  pilT Acinetobacter nosocomialis M2

51.205

97.935

0.501
  pilT Acinetobacter baylyi ADP1

50.904

97.935

0.499
  pilT Pseudomonas stutzeri DSM 10701

50.602

97.935

0.496
  pilT Pseudomonas aeruginosa PAK

49.851

98.82

0.493
  pilT Neisseria meningitidis 8013

49.701

98.525

0.49
  pilT Neisseria gonorrhoeae MS11

49.254

98.82

0.487
  pilT Legionella pneumophila strain Lp02

49.245

97.64

0.481
  pilT Legionella pneumophila strain ERS1305867

49.245

97.64

0.481
  pilU Pseudomonas stutzeri DSM 10701

44.97

99.705

0.448
  pilU Vibrio cholerae strain A1552

45.312

94.395

0.428
  pilU Acinetobacter baylyi ADP1

43.252

96.165

0.416

Multiple sequence alignment