Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MTJW_RS08005 Genome accession   NZ_CP017237
Coordinates   1572174..1573835 (-) Length   553 a.a.
NCBI ID   WP_071541855.1    Uniprot ID   -
Organism   Moorella thermoacetica strain DSM 103284     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1567174..1578835
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTJW_RS07980 (MTJW_15990) - 1567494..1568000 (-) 507 WP_071541852.1 type II secretion system protein -
  MTJW_RS07985 (MTJW_16000) - 1568047..1568790 (-) 744 WP_071541853.1 A24 family peptidase -
  MTJW_RS07990 (MTJW_16010) - 1568955..1569491 (-) 537 WP_071541854.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MTJW_RS07995 (MTJW_16020) - 1569737..1570945 (-) 1209 WP_071542514.1 type II secretion system F family protein -
  MTJW_RS08000 (MTJW_16030) pilT 1571141..1572160 (-) 1020 WP_069589960.1 type IV pilus twitching motility protein PilT Machinery gene
  MTJW_RS08005 (MTJW_16040) pilF 1572174..1573835 (-) 1662 WP_071541855.1 GspE/PulE family protein Machinery gene
  MTJW_RS08010 (MTJW_16050) aroB 1573841..1574935 (-) 1095 WP_071541856.1 3-dehydroquinate synthase -
  MTJW_RS08015 (MTJW_16060) - 1574914..1575435 (-) 522 WP_071541857.1 shikimate kinase -
  MTJW_RS08020 (MTJW_16070) aroC 1575489..1576640 (-) 1152 WP_071541858.1 chorismate synthase -
  MTJW_RS08025 (MTJW_16080) - 1576665..1577552 (-) 888 WP_071541859.1 shikimate dehydrogenase -
  MTJW_RS08030 (MTJW_16090) - 1577672..1578193 (-) 522 WP_071541860.1 YqeG family HAD IIIA-type phosphatase -

Sequence

Protein


Download         Length: 553 a.a.        Molecular weight: 60500.20 Da        Isoelectric Point: 6.2155

>NTDB_id=196807 MTJW_RS08005 WP_071541855.1 1572174..1573835(-) (pilF) [Moorella thermoacetica strain DSM 103284]
MDSRRRLGDLLIEAGMLTPAQLEQALQEQKRSGERLGKVLIRLGFITEASMLEVLEFQLGIPKVVLADYHLDPEVVRLVP
EGLARRYQAIPIRLDGNRLLVAMADPLNLVALDDLRLVTGKEIMPAIAAEKEIEAALSRFWQREPVTSMSEVAAAVAAAE
SGGRAGGTEGAPAVRLVNSFIQQAIQTRASDIHIEPQEGEVRVRLRVDGLLRELTRLPLGVLSSLISRIKIMAGMDIAEK
RLPQDGRFQFTLGKRSVDLRVSSLPTVYGEKIVLRLLDQEAMLLPLDDLGFLPAIKERFESLIHSSYGMLLITGPTGSGK
TTTLYATLNILSSPEKNIITIEDPVEYLLPGINQVRVNPKAGLTFASGLRSILRQDPDIIMVGEIRDRETADIAVRAATT
GHLVLTTLHTNDAAGAVTRLLDMGVEGYLVNSSLIGVVAQRLVRRICPHCREMYEPEPGSPERAWLPGEERLWRGRGCEN
CHYTGYANRTAIQEVLVMNEELRRLVAAKAPATALKEAAVAGGMIPLIDDGLEKARQGITTVSEVLRVSLGGL

Nucleotide


Download         Length: 1662 bp        

>NTDB_id=196807 MTJW_RS08005 WP_071541855.1 1572174..1573835(-) (pilF) [Moorella thermoacetica strain DSM 103284]
ATGGATAGTCGACGACGACTGGGGGACCTGTTAATCGAAGCCGGGATGCTTACCCCGGCCCAGCTGGAACAGGCCCTGCA
GGAACAGAAACGCAGCGGGGAGCGCCTGGGTAAGGTTTTAATCCGCCTGGGATTTATCACCGAGGCCAGCATGCTGGAGG
TCCTGGAGTTCCAGCTGGGGATCCCCAAGGTGGTCCTGGCTGACTACCACCTGGATCCGGAGGTGGTCCGCCTGGTGCCG
GAAGGCCTGGCCCGGCGCTACCAGGCCATCCCCATCCGCCTGGACGGCAACCGCCTCCTGGTGGCCATGGCCGATCCCCT
GAACCTCGTGGCCCTGGACGACCTGCGCCTGGTCACCGGCAAGGAGATTATGCCGGCTATAGCCGCCGAGAAGGAAATCG
AGGCAGCTTTAAGCCGGTTCTGGCAACGGGAACCCGTTACGAGCATGAGCGAAGTAGCGGCAGCCGTCGCCGCCGCGGAA
TCTGGCGGGCGCGCCGGCGGCACGGAAGGCGCGCCGGCTGTGCGCCTGGTCAACAGTTTTATCCAGCAGGCCATCCAGAC
CCGGGCCAGCGACATCCATATAGAGCCCCAGGAGGGGGAGGTCCGGGTGCGCCTGCGGGTAGACGGCCTGCTGCGGGAGT
TGACCCGCCTGCCCCTGGGGGTTTTAAGTAGCCTGATCTCCAGGATCAAGATCATGGCCGGCATGGACATCGCCGAAAAA
CGCTTGCCCCAGGACGGCCGTTTTCAGTTTACCCTGGGTAAACGCAGTGTAGACCTCAGGGTTTCCAGCCTGCCTACTGT
TTACGGCGAAAAGATCGTCCTGCGCCTCCTGGACCAGGAGGCCATGCTCCTGCCCCTGGACGACCTGGGATTTTTGCCGG
CCATAAAAGAACGCTTTGAGAGCCTCATCCACAGTTCCTACGGCATGCTCCTCATTACCGGTCCCACGGGCAGCGGTAAG
ACGACGACCCTCTATGCTACCCTTAACATTTTAAGCTCGCCGGAAAAAAATATCATTACCATTGAGGACCCGGTAGAATA
CCTGCTGCCCGGCATCAATCAGGTGCGGGTTAACCCCAAGGCTGGCCTGACCTTTGCTTCAGGGCTGCGTTCCATCCTGC
GCCAGGACCCGGATATCATCATGGTCGGGGAGATTCGCGACCGGGAGACGGCCGATATCGCCGTCCGGGCGGCGACTACC
GGTCATCTGGTCTTAACGACCCTGCACACCAATGACGCCGCCGGCGCCGTAACCCGCCTCCTGGATATGGGAGTGGAAGG
CTACCTGGTCAATTCCTCCCTTATTGGCGTGGTGGCCCAGCGCCTGGTGCGCCGCATCTGTCCCCATTGCCGGGAGATGT
ACGAGCCGGAGCCGGGCTCTCCGGAAAGGGCCTGGTTGCCGGGCGAGGAACGGCTCTGGCGCGGCCGGGGTTGCGAAAAC
TGCCATTATACCGGTTACGCCAACCGGACGGCCATCCAGGAGGTTCTGGTCATGAATGAAGAACTCCGGCGCCTGGTAGC
CGCCAAGGCGCCGGCTACGGCCCTGAAGGAGGCGGCGGTGGCCGGCGGTATGATTCCTTTAATCGATGACGGTTTGGAAA
AAGCCCGCCAGGGGATCACTACGGTGAGCGAGGTCCTACGCGTTTCCCTGGGAGGTTTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-144)

Type II/IV secretion system protein (T2SSE)

(180-445)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

49.18

99.277

0.488
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.273

99.458

0.45
  pilB Vibrio campbellii strain DS40M4

41.831

100

0.421
  pilB Vibrio parahaemolyticus RIMD 2210633

41.281

100

0.42
  pilB Vibrio cholerae strain A1552

41.877

100

0.42
  pilB/pilB1 Synechocystis sp. PCC 6803

38.095

100

0.42
  pilB Acinetobacter baylyi ADP1

40.541

100

0.407
  pilB Acinetobacter baumannii D1279779

39.421

100

0.394
  pilF Neisseria gonorrhoeae MS11

38.139

99.096

0.378
  pilB Legionella pneumophila strain ERS1305867

41.582

89.15

0.371

Multiple sequence alignment