Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BEN76_RS09065 Genome accession   NZ_CP016896
Coordinates   1911292..1912326 (+) Length   344 a.a.
NCBI ID   WP_004945874.1    Uniprot ID   A0A1P8EIW7
Organism   Acinetobacter soli strain GFJ2     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1906292..1917326
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEN76_RS09050 (BEN76_09050) - 1908638..1909429 (+) 792 WP_076032902.1 transglutaminase-like domain-containing protein -
  BEN76_RS09055 (BEN76_09055) - 1909524..1910369 (-) 846 WP_076032903.1 undecaprenyl-diphosphate phosphatase -
  BEN76_RS09060 (BEN76_09060) - 1910474..1911166 (-) 693 WP_076032904.1 YggS family pyridoxal phosphate-dependent enzyme -
  BEN76_RS09065 (BEN76_09065) pilT 1911292..1912326 (+) 1035 WP_004945874.1 type IV pilus twitching motility protein PilT Machinery gene
  BEN76_RS09070 (BEN76_09070) pilU 1912359..1913510 (+) 1152 WP_004945871.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BEN76_RS09075 (BEN76_09075) fur 1913561..1913998 (-) 438 WP_004935475.1 ferric iron uptake transcriptional regulator -
  BEN76_RS09080 (BEN76_09080) - 1914110..1914508 (+) 399 WP_004935473.1 outer membrane protein assembly factor BamE -
  BEN76_RS09085 (BEN76_09085) - 1914564..1914875 (-) 312 WP_076032905.1 RnfH family protein -
  BEN76_RS09090 (BEN76_09090) - 1914872..1915960 (-) 1089 WP_171261662.1 hypothetical protein -
  BEN76_RS09095 (BEN76_09095) - 1916100..1916948 (-) 849 WP_076032907.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38293.01 Da        Isoelectric Point: 6.4337

>NTDB_id=193516 BEN76_RS09065 WP_004945874.1 1911292..1912326(+) (pilT) [Acinetobacter soli strain GFJ2]
MDITELLAFSVQNKASDLHLSAGLAPMIRVDGEVRRINLPALEHSEVYRLVYDIMNDKQRRDYEEKLETDFSFEVPNLAR
FRVNVFNQNRGAGAVFRTIPSQVLTLEELGLGKIFRDICDYPRGLVLVTGPTGSGKSTTLAAMLDYINHHRYDHILTVED
PIEFVHEPKKCLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAEEKDMVRTMLSESLQAVISQTLLKKEGGGRVAAHEIMIGIPAIRNLIREGKVAQMYSSIQTGASHGMTTLDQSL
KQLVAKGIINLPTARAVAKSPESL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=193516 BEN76_RS09065 WP_004945874.1 1911292..1912326(+) (pilT) [Acinetobacter soli strain GFJ2]
ATGGATATAACCGAACTGCTGGCATTTTCAGTCCAAAACAAGGCATCTGATTTACATTTGTCTGCAGGTTTGGCACCCAT
GATTCGCGTCGATGGTGAAGTTCGACGCATTAACCTCCCCGCACTTGAACATTCCGAAGTATACCGGCTGGTTTACGACA
TTATGAATGACAAACAGCGCCGCGATTACGAAGAAAAGCTCGAAACCGACTTTTCCTTTGAGGTGCCAAATCTGGCCCGT
TTTCGTGTCAATGTGTTTAATCAGAACCGTGGTGCAGGTGCCGTTTTTCGAACCATTCCCTCACAGGTATTGACGCTTGA
AGAACTCGGACTCGGAAAAATTTTCAGAGACATCTGTGACTATCCGCGCGGCTTGGTGTTAGTGACCGGCCCAACAGGCT
CGGGGAAGTCGACCACTCTTGCCGCTATGCTCGATTATATTAATCATCATCGCTATGATCATATTTTAACGGTTGAAGAT
CCAATCGAGTTTGTACACGAACCCAAAAAATGCCTGATCAACCAGCGCGAAGTGCACCGCGATACACTTGGTTTTAATGA
GGCACTTCGCTCAGCACTGCGTGAAGATCCCGATATCATTCTGGTGGGGGAAATGCGTGATCTGGAAACCATACGTTTGG
CATTGACTGCCGCAGAAACCGGACATTTGGTGTTTGGTACGCTACATACGACCTCGGCAGCCAAAACCATTGATCGAATC
ATCGATGTATTTCCCGCAGAGGAAAAAGATATGGTTCGTACCATGCTGTCCGAATCCCTTCAGGCGGTTATTTCGCAAAC
CTTGCTAAAAAAAGAAGGTGGCGGCCGTGTAGCAGCACATGAAATTATGATTGGTATTCCGGCCATTCGTAATTTGATCC
GTGAAGGAAAAGTCGCGCAAATGTATTCTTCGATTCAAACCGGTGCAAGCCATGGCATGACTACGCTCGATCAAAGTCTT
AAACAACTGGTCGCCAAAGGCATCATTAACTTACCAACAGCACGCGCTGTGGCAAAATCACCAGAATCCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1P8EIW7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

96.512

100

0.965
  pilT Acinetobacter baumannii D1279779

89.535

100

0.895
  pilT Acinetobacter baumannii strain A118

89.535

100

0.895
  pilT Acinetobacter nosocomialis M2

89.244

100

0.892
  pilT Pseudomonas stutzeri DSM 10701

81.686

100

0.817
  pilT Pseudomonas aeruginosa PAK

81.686

100

0.817
  pilT Legionella pneumophila strain Lp02

74.852

98.256

0.735
  pilT Legionella pneumophila strain ERS1305867

74.852

98.256

0.735
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.818

95.93

0.689
  pilT Vibrio cholerae strain A1552

71.818

95.93

0.689
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.636

95.93

0.515
  pilU Vibrio cholerae strain A1552

42.029

100

0.422
  pilU Pseudomonas stutzeri DSM 10701

39.13

100

0.392
  pilU Acinetobacter baylyi ADP1

38.728

100

0.39

Multiple sequence alignment