Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BA953_RS11050 Genome accession   NZ_CP016556
Coordinates   2397013..2398119 (+) Length   368 a.a.
NCBI ID   WP_065741126.1    Uniprot ID   -
Organism   Vibrio coralliilyticus strain 58     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2392013..2403119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BA953_RS11015 (BA953_11015) - 2392215..2392817 (-) 603 WP_006958269.1 XTP/dITP diphosphatase -
  BA953_RS11020 (BA953_11020) - 2392861..2393292 (-) 432 WP_006958268.1 DUF4426 domain-containing protein -
  BA953_RS11025 (BA953_11025) yggU 2393491..2393781 (-) 291 WP_065741123.1 DUF167 family protein YggU -
  BA953_RS11030 (BA953_11030) - 2393781..2394338 (-) 558 WP_038159781.1 YggT family protein -
  BA953_RS11035 (BA953_11035) proC 2394377..2395195 (-) 819 WP_065741124.1 pyrroline-5-carboxylate reductase -
  BA953_RS11040 (BA953_11040) - 2395223..2395939 (-) 717 WP_065741125.1 YggS family pyridoxal phosphate-dependent enzyme -
  BA953_RS11045 (BA953_11045) pilT 2395964..2397001 (+) 1038 WP_043009311.1 type IV pilus twitching motility protein PilT Machinery gene
  BA953_RS11050 (BA953_11050) pilU 2397013..2398119 (+) 1107 WP_065741126.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BA953_RS11055 (BA953_11055) ruvX 2398218..2398640 (-) 423 WP_038509528.1 Holliday junction resolvase RuvX -
  BA953_RS11060 (BA953_11060) - 2398658..2399221 (-) 564 WP_065741127.1 YqgE/AlgH family protein -
  BA953_RS11065 (BA953_11065) gshB 2399278..2400228 (-) 951 WP_065741128.1 glutathione synthase -
  BA953_RS11070 (BA953_11070) rsmE 2400242..2400973 (-) 732 WP_065741129.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  BA953_RS11075 (BA953_11075) - 2401082..2401786 (-) 705 WP_065741130.1 endonuclease -
  BA953_RS11080 (BA953_11080) - 2401866..2402363 (-) 498 WP_029236317.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41212.83 Da        Isoelectric Point: 5.3077

>NTDB_id=189163 BA953_RS11050 WP_065741126.1 2397013..2398119(+) (pilU) [Vibrio coralliilyticus strain 58]
MDINAYLQGMLTEKASDLYITVGAPILYRVDGELKPHGESLSEAEVLTLLHSTMDEERQSDYSHSREANFAVVRDFGRFR
VSAFYQRELPGAVIRRIETTIPTFDDLKLPETLKDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNTERSGHILTVEDPI
EFVHEHQKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLKGVIGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMSKSQEIGMQTFDQALY
QLVVNDKITEEDALHSADSANDLRIMLKTQRGESFGGGALADVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=189163 BA953_RS11050 WP_065741126.1 2397013..2398119(+) (pilU) [Vibrio coralliilyticus strain 58]
ATGGATATCAATGCTTATCTGCAAGGCATGCTGACTGAGAAAGCGTCAGACTTATACATCACTGTTGGTGCGCCGATACT
TTATCGTGTTGATGGTGAGTTGAAGCCGCATGGAGAGAGCTTATCGGAAGCCGAAGTGCTGACGCTACTCCACAGCACCA
TGGATGAAGAGCGCCAGAGTGACTATAGCCACAGTCGAGAAGCGAACTTTGCCGTAGTGCGGGATTTTGGTCGCTTTCGT
GTGTCCGCTTTCTACCAGCGAGAGTTACCCGGTGCGGTGATTCGTAGAATCGAAACCACCATACCCACATTTGATGACTT
AAAACTGCCCGAAACTCTGAAAGACCTTTCCATTGCCAAGCGTGGTTTGGTCTTAGTTGTGGGTGCCACTGGTTCTGGTA
AATCCACAACAATGGCAGCGATGACCGGCTATCGAAATACTGAGCGCAGCGGCCATATTCTGACAGTTGAAGACCCGATT
GAATTTGTCCACGAACATCAGAAATGCATCGTCACGCAACGTGAGGTTGGCTTAGACACAGAAAGTTATGAAGTCGCGCT
GAAGAACTCTTTGCGTCAGGCTCCTGATATGATCTTGATCGGTGAGATCCGCTCGCGAGAGACCATGGAGTACGCGATGA
CATTCGCTGAGACGGGGCATCTGTGCATGGCGACGTTGCACGCTAACAATGCTAACCAAGCTTTGGAAAGGATTCTGCAT
CTTGTACCGAAAGAGCAAAAAGAGCAGTTCTTGTTTGATCTCTCCATGAATCTTAAAGGTGTTATCGGCCAGCAGTTGAT
TCGAGATAAAAACGGCCAAGGTCGTCATGGCGTTTTTGAAATCTTACTCAACAGCCCACGAGTATCGGATCTCATTCGAC
GCGGTGATCTGCATGAACTCAAATCAACCATGTCGAAATCTCAGGAAATTGGTATGCAGACGTTTGATCAGGCGCTTTAC
CAACTGGTGGTGAATGACAAAATTACTGAAGAAGATGCGCTGCACAGTGCGGATTCAGCCAATGATCTGCGTATCATGCT
TAAAACTCAACGAGGTGAAAGCTTCGGTGGTGGTGCGCTGGCAGATGTGAAGATTGATATGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.609

100

0.826
  pilU Pseudomonas stutzeri DSM 10701

57.429

95.109

0.546
  pilU Acinetobacter baylyi ADP1

52.707

95.38

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.248

92.12

0.408
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Acinetobacter baylyi ADP1

41.935

92.663

0.389
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.118

92.12

0.37
  pilT Neisseria meningitidis 8013

39.13

93.75

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.538

88.315

0.367
  pilT Neisseria gonorrhoeae MS11

39.13

93.75

0.367
  pilT Vibrio cholerae strain A1552

41.538

88.315

0.367

Multiple sequence alignment