Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BA953_RS11045 Genome accession   NZ_CP016556
Coordinates   2395964..2397001 (+) Length   345 a.a.
NCBI ID   WP_043009311.1    Uniprot ID   A0AAN0VXY8
Organism   Vibrio coralliilyticus strain 58     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2390964..2402001
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BA953_RS11010 (BA953_11010) hemW 2391037..2392215 (-) 1179 WP_065741122.1 radical SAM family heme chaperone HemW -
  BA953_RS11015 (BA953_11015) - 2392215..2392817 (-) 603 WP_006958269.1 XTP/dITP diphosphatase -
  BA953_RS11020 (BA953_11020) - 2392861..2393292 (-) 432 WP_006958268.1 DUF4426 domain-containing protein -
  BA953_RS11025 (BA953_11025) yggU 2393491..2393781 (-) 291 WP_065741123.1 DUF167 family protein YggU -
  BA953_RS11030 (BA953_11030) - 2393781..2394338 (-) 558 WP_038159781.1 YggT family protein -
  BA953_RS11035 (BA953_11035) proC 2394377..2395195 (-) 819 WP_065741124.1 pyrroline-5-carboxylate reductase -
  BA953_RS11040 (BA953_11040) - 2395223..2395939 (-) 717 WP_065741125.1 YggS family pyridoxal phosphate-dependent enzyme -
  BA953_RS11045 (BA953_11045) pilT 2395964..2397001 (+) 1038 WP_043009311.1 type IV pilus twitching motility protein PilT Machinery gene
  BA953_RS11050 (BA953_11050) pilU 2397013..2398119 (+) 1107 WP_065741126.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BA953_RS11055 (BA953_11055) ruvX 2398218..2398640 (-) 423 WP_038509528.1 Holliday junction resolvase RuvX -
  BA953_RS11060 (BA953_11060) - 2398658..2399221 (-) 564 WP_065741127.1 YqgE/AlgH family protein -
  BA953_RS11065 (BA953_11065) gshB 2399278..2400228 (-) 951 WP_065741128.1 glutathione synthase -
  BA953_RS11070 (BA953_11070) rsmE 2400242..2400973 (-) 732 WP_065741129.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  BA953_RS11075 (BA953_11075) - 2401082..2401786 (-) 705 WP_065741130.1 endonuclease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38098.45 Da        Isoelectric Point: 6.1084

>NTDB_id=189162 BA953_RS11045 WP_043009311.1 2395964..2397001(+) (pilT) [Vibrio coralliilyticus strain 58]
MDIAELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGVPAFNHSEVHRLVFEIMNDAQRSEFEERLEVDFSFALPDVGR
FRVNAFNQSRGCAAVFRTIPTDIPTLEQLEAPSIFENIANMEKGLILVTGPTGSGKSTTLAAMVDHINRNHNKHILTIED
PIEFVHTNNKCLINQREVHNDTHSFNNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKGMVRSMLSESLRAVIAQKLLKRIGGGRTACHEIMMATPAIRNLIREDKVAQMQSVIQTGAAHGMQTMEQHS
KQLIAQGLVDAEEVSKKIEVEQGLF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=189162 BA953_RS11045 WP_043009311.1 2395964..2397001(+) (pilT) [Vibrio coralliilyticus strain 58]
ATGGATATCGCTGAATTACTGGATTTTAGTGTAAAGCATAATGCTTCAGATCTACATCTTTCTGCTGGTGTATCTCCCAT
GGTACGCATTGATGGTGAAGTAAGAAAGCTTGGCGTACCAGCGTTCAACCATTCCGAAGTGCATCGTTTGGTGTTTGAAA
TTATGAACGATGCTCAGCGTAGCGAATTTGAAGAACGTCTTGAAGTCGACTTTTCTTTTGCACTTCCGGACGTTGGCCGT
TTTAGGGTCAACGCTTTTAACCAATCCCGTGGTTGTGCGGCCGTATTTCGTACCATTCCAACTGATATCCCGACTTTAGA
GCAGCTAGAAGCGCCGTCGATCTTTGAAAATATCGCCAATATGGAGAAAGGATTAATTTTGGTGACAGGCCCGACAGGCT
CAGGTAAATCGACTACTTTGGCGGCAATGGTGGATCATATTAATCGCAACCACAATAAGCATATCCTGACTATTGAAGAT
CCGATTGAGTTTGTGCACACCAATAACAAATGTCTGATCAACCAGCGTGAAGTTCACAATGATACCCATAGTTTTAATAA
CGCGCTGCGCAGTGCATTGCGTGAAGATCCCGATGTCATTTTAGTCGGTGAGCTACGTGATCAGGAAACGATCAGTCTAG
CGCTGACCGCTGCTGAAACTGGCCACTTGGTATTTGGTACTTTGCACACCAGTTCGGCGGCGAAAACTATCGACCGTATC
ATTGATGTTTTCCCGGGTAGCGACAAAGGCATGGTGCGTTCCATGCTCTCTGAGTCTCTGCGTGCGGTGATTGCGCAGAA
ATTACTCAAGCGCATTGGCGGCGGGAGAACCGCTTGTCATGAAATCATGATGGCAACGCCTGCTATTCGTAATCTGATCC
GCGAAGACAAAGTCGCACAAATGCAGTCTGTGATTCAGACTGGTGCGGCGCATGGTATGCAAACCATGGAGCAGCACTCC
AAACAGTTGATTGCTCAAGGTCTGGTTGATGCAGAAGAAGTGTCGAAGAAAATTGAAGTTGAGCAAGGTCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

86.957

100

0.87
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.957

100

0.87
  pilT Acinetobacter baylyi ADP1

68.421

99.13

0.678
  pilT Acinetobacter baumannii D1279779

69.578

96.232

0.67
  pilT Acinetobacter baumannii strain A118

69.578

96.232

0.67
  pilT Acinetobacter nosocomialis M2

69.277

96.232

0.667
  pilT Pseudomonas stutzeri DSM 10701

66.766

97.681

0.652
  pilT Pseudomonas aeruginosa PAK

65.497

99.13

0.649
  pilT Legionella pneumophila strain Lp02

66.869

95.362

0.638
  pilT Legionella pneumophila strain ERS1305867

66.869

95.362

0.638
  pilT Neisseria meningitidis 8013

64.497

97.971

0.632
  pilT Neisseria gonorrhoeae MS11

63.235

98.551

0.623
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.818

95.652

0.496
  pilU Vibrio cholerae strain A1552

42.154

94.203

0.397
  pilU Pseudomonas stutzeri DSM 10701

40.419

96.812

0.391
  pilU Acinetobacter baylyi ADP1

40.938

92.754

0.38

Multiple sequence alignment