Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   vm_RS13050 Genome accession   NZ_CP016383
Coordinates   2794996..2796033 (-) Length   345 a.a.
NCBI ID   WP_000350205.1    Uniprot ID   D2YE46
Organism   Vibrio mimicus strain SCCF01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2789996..2801033
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  vm_RS13025 (VM_13030) - 2790479..2791042 (+) 564 WP_005526767.1 YqgE/AlgH family protein -
  vm_RS13030 (VM_13035) ruvX 2791123..2791545 (+) 423 WP_000091871.1 Holliday junction resolvase RuvX -
  vm_RS13035 (VM_13040) tyrS 2791570..2792850 (-) 1281 WP_070382341.1 tyrosine--tRNA ligase -
  vm_RS13040 (VM_13045) - 2792959..2793756 (-) 798 WP_070382613.1 helix-turn-helix transcriptional regulator -
  vm_RS13045 (VM_13050) pilU 2793875..2794981 (-) 1107 WP_070382342.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  vm_RS13050 (VM_13055) pilT 2794996..2796033 (-) 1038 WP_000350205.1 type IV pilus twitching motility protein PilT Machinery gene
  vm_RS13055 (VM_13060) - 2796059..2796769 (+) 711 WP_070382343.1 YggS family pyridoxal phosphate-dependent enzyme -
  vm_RS13060 (VM_13065) proC 2796824..2797642 (+) 819 WP_070382344.1 pyrroline-5-carboxylate reductase -
  vm_RS13065 (VM_13070) - 2797700..2798257 (+) 558 WP_005509546.1 YggT family protein -
  vm_RS13070 (VM_13075) yggU 2798257..2798550 (+) 294 WP_000741913.1 DUF167 family protein YggU -
  vm_RS13075 (VM_13080) - 2798644..2799075 (+) 432 WP_070382345.1 DUF4426 domain-containing protein -
  vm_RS13080 (VM_13085) - 2799161..2799763 (+) 603 WP_070382346.1 XTP/dITP diphosphatase -
  vm_RS13085 (VM_13090) hemW 2799757..2800941 (+) 1185 WP_022577539.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38248.81 Da        Isoelectric Point: 6.5572

>NTDB_id=187701 vm_RS13050 WP_000350205.1 2794996..2796033(-) (pilT) [Vibrio mimicus strain SCCF01]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLVFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQSRGCSAVFRTIPTVIPTLDQLEAPEIFTKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIARGVVDAQEVQSKIELDLKAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=187701 vm_RS13050 WP_000350205.1 2794996..2796033(-) (pilT) [Vibrio mimicus strain SCCF01]
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCAGGTGTTCCGCCTAT
GGTACGGATTGATGGTGAAGTTAGGAAGCTCGGCGTGCCTGCTTTTACTCATTCTGACGTACATCGCTTGGTATTTGAAA
TAATGAATGATGCTCAGCGCAGTGAATATGAAGAAAAATTAGAAGTCGATTTTTCTTTTGAATTACCCAACGTTGGCCGC
TTCCGGGTTAACGCGTTTCACCAATCGCGCGGATGCTCTGCGGTATTTCGTACCATTCCGACGGTGATCCCGACGTTAGA
TCAGCTTGAAGCTCCTGAGATTTTTACCAAGATCGCCAATTATGAAAAAGGCTTAGTGCTGGTCACTGGACCTACCGGTT
CGGGTAAATCCACCACGCTCGCGGCGATGGTTGATTACGTGAATGCCCACCATAACAAGCACATTTTGACCATTGAAGAT
CCGATTGAATTTGTACACAGCAACAATAAGTGTTTGATCAACCAACGCGAAGTGCACCGTGATACCCATAGTTTTAAAAA
TGCCCTGCGCTCGGCATTGCGTGAAGACCCAGATGTGATTCTGGTTGGTGAGCTGCGTGACCAAGAAACCATTAGCTTGG
CGCTTACCGCGGCAGAAACGGGTCACTTGGTGTTTGGTACTCTGCACACCAGCTCGGCGGCAAAAACCATCGACCGTATT
ATCGACGTTTTTCCAGGCAGTGATAAAGACATGGTGCGTTCAATGCTTTCTGAATCTCTGCGTGCAGTTATCGCGCAAAA
GCTCTTAAAACGCGTGGGTGGCGGTCGTGTCGCGTGCCATGAAATTATGCTGGCGACTCCGGCAATCCGTAACTTGATCC
GTGAAGATAAGGTGGCGCAGATGTATTCGATCATCCAAACCGGTGCAGCGCATGGTATGCAGACGATGGAGCAAAATGCG
AAGCAGCTGATTGCGCGTGGCGTAGTGGATGCGCAAGAAGTACAGAGCAAAATCGAGTTGGATTTAAAAGCATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D2YE46

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

98.261

100

0.983
  pilT Vibrio cholerae strain A1552

98.261

100

0.983
  pilT Acinetobacter baumannii D1279779

72.024

97.391

0.701
  pilT Acinetobacter baumannii strain A118

72.024

97.391

0.701
  pilT Acinetobacter nosocomialis M2

71.726

97.391

0.699
  pilT Acinetobacter baylyi ADP1

72.121

95.652

0.69
  pilT Pseudomonas stutzeri DSM 10701

68.546

97.681

0.67
  pilT Pseudomonas aeruginosa PAK

66.471

98.551

0.655
  pilT Legionella pneumophila strain Lp02

67.477

95.362

0.643
  pilT Legionella pneumophila strain ERS1305867

67.477

95.362

0.643
  pilT Neisseria meningitidis 8013

64.242

95.652

0.614
  pilT Neisseria gonorrhoeae MS11

63.939

95.652

0.612
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.294

94.783

0.496
  pilU Vibrio cholerae strain A1552

41.916

96.812

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.812

0.388
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377

Multiple sequence alignment