Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   vm_RS13045 Genome accession   NZ_CP016383
Coordinates   2793875..2794981 (-) Length   368 a.a.
NCBI ID   WP_070382342.1    Uniprot ID   -
Organism   Vibrio mimicus strain SCCF01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2788875..2799981
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  vm_RS13020 (VM_13025) gshB 2789487..2790443 (+) 957 WP_070382340.1 glutathione synthase -
  vm_RS13025 (VM_13030) - 2790479..2791042 (+) 564 WP_005526767.1 YqgE/AlgH family protein -
  vm_RS13030 (VM_13035) ruvX 2791123..2791545 (+) 423 WP_000091871.1 Holliday junction resolvase RuvX -
  vm_RS13035 (VM_13040) tyrS 2791570..2792850 (-) 1281 WP_070382341.1 tyrosine--tRNA ligase -
  vm_RS13040 (VM_13045) - 2792959..2793756 (-) 798 WP_070382613.1 helix-turn-helix transcriptional regulator -
  vm_RS13045 (VM_13050) pilU 2793875..2794981 (-) 1107 WP_070382342.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  vm_RS13050 (VM_13055) pilT 2794996..2796033 (-) 1038 WP_000350205.1 type IV pilus twitching motility protein PilT Machinery gene
  vm_RS13055 (VM_13060) - 2796059..2796769 (+) 711 WP_070382343.1 YggS family pyridoxal phosphate-dependent enzyme -
  vm_RS13060 (VM_13065) proC 2796824..2797642 (+) 819 WP_070382344.1 pyrroline-5-carboxylate reductase -
  vm_RS13065 (VM_13070) - 2797700..2798257 (+) 558 WP_005509546.1 YggT family protein -
  vm_RS13070 (VM_13075) yggU 2798257..2798550 (+) 294 WP_000741913.1 DUF167 family protein YggU -
  vm_RS13075 (VM_13080) - 2798644..2799075 (+) 432 WP_070382345.1 DUF4426 domain-containing protein -
  vm_RS13080 (VM_13085) - 2799161..2799763 (+) 603 WP_070382346.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41337.39 Da        Isoelectric Point: 7.1149

>NTDB_id=187700 vm_RS13045 WP_070382342.1 2793875..2794981(-) (pilU) [Vibrio mimicus strain SCCF01]
MELNQYLDGMLSHKASDLYITVGAPILYRVDGELRAQGEVLSLADVTTLLHAMMDDARQAEFKQTREANFAVVRESGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPAVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMQYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIGQQLLRDKNGKGRHGVFEVLLNSPRVADLIRRGDLHELKTTMARSKEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDNYGSSSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=187700 vm_RS13045 WP_070382342.1 2793875..2794981(-) (pilU) [Vibrio mimicus strain SCCF01]
ATGGAGTTGAATCAATATCTGGATGGGATGCTGAGCCATAAAGCATCGGATCTTTACATCACCGTTGGAGCGCCGATTTT
ATATCGGGTCGATGGTGAATTGCGCGCGCAAGGTGAAGTGCTCAGTTTGGCGGATGTGACGACCTTACTGCATGCGATGA
TGGATGACGCGAGACAAGCGGAATTTAAGCAGACGCGTGAAGCGAACTTTGCTGTAGTACGCGAAAGTGGCCGTTTCCGG
GTTAGTGCGTTTTTCCAACGTGAATTGCCGGGCGCGGTGATCCGCCGGATTGAAACGCGTATTCCCACCTTTGAAGAGCT
GAAATTACCCGCGGTACTGCAAAATTTAGCGATCGCGAAACGTGGTTTAGTCTTAGTAGTGGGTGCTACGGGGTCAGGTA
AATCGACCACTATGGCGGCAATGACGGGGTATCGTAACCAACACCGTACTGGACATATCTTGACGGTCGAAGACCCGATT
GAGTTTGTGCATGAACATAAGCGTTGTATCGTCACCCAACGAGAAGTCGGCTTAGATACTGAAAGCTATGAAGTGGCGTT
GAAAAACTCACTGCGCCAAGCACCGGATATGATTTTGATTGGTGAAATTCGCAGCCGAGAAACCATGCAATACGCGATGA
CCTTCGCGGAAACGGGGCATTTGTGTATGGCCACACTGCACGCCAACAACGCCAACCAAGCTTTAGAGCGGATTTTGCAT
TTAGTGCCGAAAGAACAGCGTGAGCAGTTCTTGCTCGACTTGTCACTCAACCTAAAAGGGGTGATTGGCCAGCAATTGTT
GCGCGATAAAAACGGCAAAGGGCGGCATGGGGTGTTTGAAGTTCTGCTCAATAGCCCACGAGTCGCGGATTTGATTCGCC
GTGGCGATCTGCATGAACTTAAAACGACCATGGCGCGTTCCAAAGAAGTTGGCATGCAGACTTTTGACCAAGCCTTGTAC
CAACTGGTGGTGGACGACAAGATTAGCGAGCAAGATGCCCTGCACAGTGCGGATTCGGCCAACGATTTGCGTTTGATGCT
CAAAACCAAGCGTGGTGACAACTACGGCAGTAGTTCTTTGCAGAATGTGAAGATTGATATGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

96.196

100

0.962
  pilU Pseudomonas stutzeri DSM 10701

58.286

95.109

0.554
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Legionella pneumophila strain Lp02

41.888

92.12

0.386
  pilT Legionella pneumophila strain ERS1305867

41.888

92.12

0.386
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.806

84.239

0.386
  pilT Acinetobacter nosocomialis M2

41.003

92.12

0.378
  pilT Acinetobacter baumannii D1279779

41.003

92.12

0.378
  pilT Acinetobacter baumannii strain A118

41.003

92.12

0.378
  pilT Vibrio cholerae strain A1552

41.018

90.761

0.372
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

90.761

0.372
  pilT Pseudomonas aeruginosa PAK

40.413

92.12

0.372
  pilT Acinetobacter baylyi ADP1

41.846

88.315

0.37
  pilT Pseudomonas stutzeri DSM 10701

39.823

92.12

0.367
  pilT Neisseria gonorrhoeae MS11

38.329

94.293

0.361
  pilT Neisseria meningitidis 8013

38.551

93.75

0.361

Multiple sequence alignment